“…Cells were grown for 2 days on 35-mm glass dishes (MatTek, Ashland, MA) maintained at 37°C and 5% CO 2 in a humidified chamber on the microscope stage (Carl Zeiss, Thornwood, NY). NAD(P)H intensity was imaged by using a ϫ40, 1.3-NA oil immersion lens (Carl Zeiss), a 710-nm mode-locked Ti:Saph laser (Ϸ3.5 mW at the sample) (Coherent, Santa Clara, CA), and fluorescence collection through a nondescanned detector with a custom 380-to 550-nm filter (Chroma, Rockingham, VT) (48). Images were analyzed with Metamorph 5.0 (Molecular Devices, Sunnyvale, CA).…”