Quantitative N‐ or C‐Terminal Labelling of Proteins with Unactivated Peptides by Use of Sortases and a d‐Aminopeptidase
Zoe L. P. Arnott,
Holly E. Morgan,
Kristian Hollingsworth
et al.
Abstract:Quantitative and selective labelling of proteins is widely used in both academic and industrial laboratories, and catalytic labelling of proteins using transpeptidases such as sortases has proved to be a popular strategy for selective modification. A major challenge for this class of enzymes is that the majority of procedures require an excess of the labelling reagent or, alternatively, activated substrates rather than simple commercially‐sourced peptides. We report the use of a coupled enzyme strategy which e… Show more
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