Quantitative multi‐spectral oxygen saturation measurements independent of tissue optical properties

Radrich, Karin; Ntziachristos, Vasilis

doi:10.1002/jbio.201400092

Cited by 6 publications

(3 citation statements)

References 44 publications

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“…Spectral reflectance was clearly different with a double-peak between 550 and 590 nm and higher values at 680 nm for physiological tissue compared to non-perfused gastric tissue as seen in Figure 2. These findings are in line with the descriptions of optical properties of oxygenated and deoxygenated hemoglobin in current literature [36][37][38] . This initial analysis serves as a reference point for the subsequent experiments as spectral reflectance of the critically perfused areas on the gastric conduit next to the stapling site is expected to be similar compared to the spectral reflectance of non-perfused gastric tissue.…”

Section: Discussionsupporting

confidence: 92%

Optimization of anastomotic technique and gastric conduit perfusion with hyperspectral imaging in an experimental model for minimally invasive esophagectomy

Nickel

Studier-Fischer

Oezdemir

et al. 2021

Preprint

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Objective: To optimize anastomotic technique and gastric conduit perfusion with hyperspectral imaging (HSI) for total minimally invasive esophagectomy (MIE) with linear stapled anastomosis. Summary Background Data: Esophagectomy is the mainstay of esophageal cancer treatment but anastomotic insufficiency related morbidity and mortality remain challenging for patient outcome. Methods: A live porcine model (n=50) for MIE was used with gastric conduit formation and linear stapled side-to-side esophagogastrostomy. Four main experimental groups differed in stapling length (3 vs. 6 cm) and anastomotic position on the conduit (cranial vs. caudal). Tissue oxygenation around the anastomotic site was evaluated using HSI and was validated with histopathology. Results: The tissue oxygenation (ΔStO2) after the anastomosis remained constant only for the short stapler in caudal position (-0.4±4.4%, n.s.) while it dropped markedly in the other groups (short-cranial: -15.6±11.5%, p=0.0002; long-cranial: -20.4±7.6%, p=0.0126; long-caudal: -16.1±9.4%, p<0.0001) Tissue samples from deoxygenated stomach as measured by HSI showed correspondent eosinophilic pre-necrotic changes in 35.7±9.7% of the surface area. Conclusions: Tissue oxygenation at the anastomotic site of the gastric conduit during MIE is influenced by stapling technique. Optimal oxygenation was achieved with a short stapler (3 cm) and sufficient distance of the anastomosis to the cranial end of the gastric conduit. HSI tissue deoxygenation corresponded to histopathologic necrotic tissue changes. These findings allow for optimization of gastric conduit perfusion and anastomotic technique in MIE.

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Section: Discussionsupporting

confidence: 92%

Optimization of anastomotic technique and gastric conduit perfusion with hyperspectral imaging in an experimental model for minimally invasive esophagectomy

Nickel

Studier-Fischer

Oezdemir

et al. 2021

Preprint

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“…To eliminate cross talk between HbO and oxCCO, ∆HbO was estimated as ∆HbT − ∆Hb instead of being fit independently. Fluctuations in HbT (∆HbT ) were estimated using Equation (2) at the isosbestic point (797 nm 18 ) before fitting ∆µ a (λ) with Equation (1).…”

Section: Data Processing and Analysismentioning

confidence: 99%

Hyperspectral time-resolved compressive sensing spectroscopy for monitoring cytochrome-c-oxidase and blood oxygenation

Li¹,

Ioussoufovitch²,

Diop

2023

Optical Tomography and Spectroscopy of Tissue XV

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Time-resolved (TR) near-infrared spectroscopy (NIRS) is a promising technique for neuromonitoring, but there are currently very few TR-NIRS devices with the spectral range and resolution needed for accurate monitoring of cerebral blood oxygenation (S t O 2 ) and metabolism (cytochrome-c-oxidase; oxCCO). Here we present a hyperspectral TR compressive sensing spectrometer with a wide spectral range, high spectral resolution, and no afterpulsing. A homogeneous blood-yeast phantom experiment was performed to evaluate the spectrometer's ability to monitor S t O 2 and oxCCO with and without compression. The effect of using a 90% compression rate on the recovered changes in deoxyhemoglobin (Hb), oxyhemoglobin (HbO), and oxCCO concentrations was investigated. No meaningful differences were found between concentration changes recovered from uncompressed and compressed data, with mean differences of 0.16 ± 0.20 µM , -0.25 ± 0.21 µM , and -0.04 ± 0.10 µM for Hb, HbO, and oxCCO, respectively. The results show that changes in oxCCO and S t O 2 can be reliably monitored with a high compression rate. Future work will compare the performance of the TR spectrometer with that of a continuous-wave spectrometer to assess accuracy and will investigate the sensitivity of the device to oxCCO and S t O 2 changes in the bottom compartment of a 2-layer tissue-mimicking phantom.

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“…An increasingly popular tool for imaging the cerebrovascular structure of the mammalian cortex is two-photon laser scanning microscopy (TPLSM) 13 . Other techniques for assessing physiological parameters include laser speckle contrast imaging (LSCI) 6 and hyperspectral imaging (HSI) 14 , 15 , which are used for monitoring hemodynamic changes in CBF 6 , 7 and SO 2 14 , 16 , 17 , respectively.…”

Section: Introductionmentioning

confidence: 99%

A large, switchable optical clearing skull window for cerebrovascular imaging

et al. 2018

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Rationale: Intravital optical imaging is a significant method for investigating cerebrovascular structure and function. However, its imaging contrast and depth are limited by the turbid skull. Tissue optical clearing has a great potential for solving this problem. Our goal was to develop a transparent skull window, without performing a craniotomy, for use in assessing cerebrovascular structure and function.Methods: Skull optical clearing agents were topically applied to the skulls of mice to create a transparent window within 15 min. The clearing efficacy, repeatability, and safety of the skull window were then investigated.Results: Imaging through the optical clearing skull window enhanced both the contrast and the depth of intravital imaging. The skull window could be used on 2-8-month-old mice and could be expanded from regional to bi-hemispheric. In addition, the window could be repeatedly established without inducing observable inflammation and metabolic toxicity.Conclusion: We successfully developed an easy-to-handle, large, switchable, and safe optical clearing skull window. Combined with various optical imaging techniques, cerebrovascular structure and function can be observed through this optical clearing skull window. Thus, it has the potential for use in basic research on the physiopathologic processes of cortical vessels.

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Quantitative multi‐spectral oxygen saturation measurements independent of tissue optical properties

Cited by 6 publications

References 44 publications

Optimization of anastomotic technique and gastric conduit perfusion with hyperspectral imaging in an experimental model for minimally invasive esophagectomy

Optimization of anastomotic technique and gastric conduit perfusion with hyperspectral imaging in an experimental model for minimally invasive esophagectomy

Hyperspectral time-resolved compressive sensing spectroscopy for monitoring cytochrome-c-oxidase and blood oxygenation

A large, switchable optical clearing skull window for cerebrovascular imaging

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