Quantitative Methods for the Study of Vitamin D

Nair, Padmanabhan P.

doi:10.1016/b978-1-4831-9940-5.50013-2

Cited by 12 publications

(2 citation statements)

References 69 publications

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“…has been the subject of several complete reviews (192,436,659). Specific methods have been described, such as chromatography of tricarboxylic acid cycle compounds (67,291,435), plant alkaloids (311), amino acids on starch (74,481) (608), phosphate esters from erythrocytes (147), methylated tRNA (62), tetracyclines (23), imino acids (71), macroglobulins (51), and antibiotics (421).…”

Section: Thin-layer Chromatography (Tlc)mentioning

confidence: 99%

Biochemical analysis

D'Eustachio¹

1968

Anal. Chem.

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This review will discuss most of the significant developments in biochemical analysis which appeared in the literature during the period from January 1966 through December 1967. Undoubtedly, many worthwhile contributions have been overlooked, but scanning the literature quickly brings one to the conclusion that the field of biochemical analysis is undergoing a "publication explosion." The deluge of pertinent reports encompasses journals in chemistry, biology, physics, electronics, and engineering; thus, the sheer physical volume demands that this review be selective, rather than comprehensive, and precludes incorporation of routine applications or minor modifications of accepted techniques and methods. This approach is in keeping with the philosophy of the previous reviews of the same title, as established by Dr. P. B. Hamilton, and appears to be well suited for the continuity of this series. Accordingly, the emphasis will be on new developments and trends having potential applications. NEW BOOKS AND JOURNALSRecent texts pertaining entirely to the subjects discussed in the following sections will be mentioned as they arise. Of general interest to biochemists will be an "Encyclopedia of Biochemistry" (663), which stresses methodology in relation to the rapid developments in this science, and an annual series "Essays in Biochemistry" (108, 109). The first volume of an "Atlas of Clinical Laboratory Procedures" (405) was published and may well be the primary reference work for clinical biochemists.Specialized fields of biochemical analysis have been treated in the con-100 was described (238). Many nu-

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Section: Thin-layer Chromatography (Tlc)mentioning

confidence: 99%

Biochemical analysis

D'Eustachio¹

1968

Anal. Chem.

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“…Absorbance at 282 nm was corrected by a modified Honda equation (14; see below). 7-DHC in the ethanolic stock solution was determined using ε293 nm EtOH = 6.74 x 10 3 M -1 cm -1 (16).…”

Section: Uv Determination Of 7-dhcmentioning

confidence: 99%

Diagnosis of Smith-Lemli-Opitz syndrome by ultraviolet spectrophotometry

Scalco

Cruzes

Vendramini

et al. 2003

Braz J Med Biol Res

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Smith-Lemli-Opitz syndrome (SLOS) is an autosomal recessive disorder due to an inborn error of cholesterol metabolism, characterized by congenital malformations, dysmorphism of multiple organs, mental retardation and delayed neuropsychomotor development resulting from cholesterol biosynthesis deficiency. A defect in 3ß-hydroxysteroid-∆ 7 -reductase (∆ 7 -sterol-reductase), responsible for the conversion of 7-dehydrocholesterol (7-DHC) to cholesterol, causes an increase in 7-DHC and frequently reduces plasma cholesterol levels. The clinical diagnosis of SLOS cannot always be conclusive because of the remarkable variability of clinical expression of the disorder. Thus, confirmation by the measurement of plasma 7-DHC levels is needed. In the present study, we used a simple, fast, and selective method based on ultraviolet spectrophotometry to measure 7-DHC in order to diagnose SLOS. 7-DHC was extracted serially from 200 µl plasma with ethanol and n-hexane and the absorbance at 234 and 282 nm was determined. The method was applied to negative control plasma samples from 23 normal individuals and from 6 cases of suspected SLOS. The method was adequate and reliable and 2 SLOS cases were diagnosed.

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