Quantitative metabolome analysis of boiled chicken egg yolk

Research Note: Changes in chicken egg yolk metabolome during its formation

“…After homogenization (MM400, Retsch, Germany) for 3 min, the beads were removed, and the tube was vortexed for 1 min, then centrifuged (5424R, Eppendorf, Germany) 12,000 × g for 10 min at 4°C. The supernatant was taken for the following LC-MS/MS analysis ( Wang et al, 2023 ).…”

“…The temperature of metabolites ionization in the sample was 550°C, and the voltage of electrospray ionization ion source was 5,500 V for positive mode or −4,500 V for negative mode. Collision-induced ionization parameters were set as high, and each ion pair in QTRAP was scanned and identified by the optimized declustering potential and collision energy ( Wang et al, 2023 ).…”

“…Based on the self-built target standard product database MWDB (Metware Biotechnology Co., Ltd., Wuhan, China), qualitative analysis was carried out based on the comprehensive determination of the daughter and parent ion pair information, the secondary spectrum data, and the detected substance retention time. The quantification of metabolites was accomplished by using the multiple reaction monitoring mode of triple quadrupole mass spectrometry ( Wang et al, 2023 ).…”

“…In addition to protein and lipids, egg yolks are rich in vitamins and small molecule metabolites, such as lutein, retinol, etc. ( Wang et al, 2023 ). These vitamins and small molecule metabolites are essential for the development of chicken embryos and are also important nutrients required by the human diet.…”

Comparative Metabolomic Profiling of Eggs from 3 Diverse Chicken Breeds Using GC-MS Analysis

“…Metabolomics, a comprehensive analysis of small molecules present in biological samples, has provided unprecedented insights into the diverse metabolic pathways inherent in various food items. Among these, eggs stand out as a quintessential source of essential nutrients, offering a rich amalgamation of proteins, lipids, vitamins, and minerals crucial for human health ( Wang et al, 2023 ). The importance of genetic factors in determining egg composition has been the interest of various research highlighting significant breed-specific differences in egg metabolite profiles.…”