Quantitative mechanics of 3D printed nanopillars interacting with bacterial cells

Ganjian, Mahya; Angeloni, Livia; Mirzaali, Mohammad J.; Modaresifar, Khashayar; Hagen, C. W.; Ghatkesar, Murali Krishna; Hagedoorn, Peter‐Leon; Fratila‐Apachitei, Lidy E.; Zadpoor, Amir A.

doi:10.1039/d0nr05984f

Cited by 16 publications

(12 citation statements)

References 62 publications

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“…[ 41 ] Patterns that lie within a specific range of dimensions have been shown to mechanically kill different types of bacteria through a combination of different mechanisms. [ 24,42,43 ]…”

Section: Introductionmentioning

confidence: 99%

On the Use of Black Ti as a Bone Substituting Biomaterial: Behind the Scenes of Dual‐Functionality

Modaresifar

Ganjian

Angeloni

et al. 2021

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Despite the potential of small‐scale pillars of black titanium (bTi) for killing the bacteria and directing the fate of stem cells, not much is known about the effects of the pillars’ design parameters on their biological properties. Here, three distinct bTi surfaces are designed and fabricated through dry etching of the titanium, each featuring different pillar designs. The interactions of the surfaces with MC3T3‐E1 preosteoblast cells and Staphylococcus aureus bacteria are then investigated. Pillars with different heights and spatial organizations differently influence the morphological characteristics of the cells, including their spreading area, aspect ratio, nucleus area, and cytoskeletal organization. The preferential formation of focal adhesions (FAs) and their size variations also depend on the type of topography. When the pillars are neither fully separated nor extremely tall, the colocalization of actin fibers and FAs as well as an enhanced matrix mineralization are observed. However, the killing efficiency of these pillars against the bacteria is not as high as that of fully separated and tall pillars. This study provides a new perspective on the dual‐functionality of bTi surfaces and elucidates how the surface design and fabrication parameters can be used to achieve a surface topography with balanced bactericidal and osteogenic properties.

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“…[ 41 ] Patterns that lie within a specific range of dimensions have been shown to mechanically kill different types of bacteria through a combination of different mechanisms. [ 24,42,43 ]…”

Section: Introductionmentioning

confidence: 99%

On the Use of Black Ti as a Bone Substituting Biomaterial: Behind the Scenes of Dual‐Functionality

Modaresifar

Ganjian

Angeloni

et al. 2021

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“…That is partially due to the difficulties associated with the fabrication of surfaces with precise micro-/nanoscale surface ornaments. Advanced micro-and nanofabrication techniques, such as 3D nanoprinting (e.g., two-photon polymerization (2PP), 20,21 electron beam induced deposition (EBID) 22 ), and lithography-based techniques (e.g., electron beam lithography (EBL), 23 nanoimprint lithography (NIL), 24 and focused ion beam lithography (FIBL) 25 ), that have been relatively recently applied for biomedical applications, 1 have offered a path forward.…”

Section: Introductionmentioning

confidence: 99%

3D printed submicron patterns orchestrate the response of macrophages

et al. 2021

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“…The killing efficiencies of different types of bacteria (e.g., Escherichia coli and Staphylococcus aureus ) in relation to various types of nano-pattern distributions have been analysed [ 419 , 420 , 421 ]. The mechano-bactericidal effects of such nano-pillars have also been investigated using atomic force microscopy [ 422 ].…”

Section: Am Of Biomedical Polymersmentioning

confidence: 99%

Additive Manufacturing of Biomaterials—Design Principles and Their Implementation

et al. 2022

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Additive manufacturing (AM, also known as 3D printing) is an advanced manufacturing technique that has enabled progress in the design and fabrication of customised or patient-specific (meta-)biomaterials and biomedical devices (e.g., implants, prosthetics, and orthotics) with complex internal microstructures and tuneable properties. In the past few decades, several design guidelines have been proposed for creating porous lattice structures, particularly for biomedical applications. Meanwhile, the capabilities of AM to fabricate a wide range of biomaterials, including metals and their alloys, polymers, and ceramics, have been exploited, offering unprecedented benefits to medical professionals and patients alike. In this review article, we provide an overview of the design principles that have been developed and used for the AM of biomaterials as well as those dealing with three major categories of biomaterials, i.e., metals (and their alloys), polymers, and ceramics. The design strategies can be categorised as: library-based design, topology optimisation, bio-inspired design, and meta-biomaterials. Recent developments related to the biomedical applications and fabrication methods of AM aimed at enhancing the quality of final 3D-printed biomaterials and improving their physical, mechanical, and biological characteristics are also highlighted. Finally, examples of 3D-printed biomaterials with tuned properties and functionalities are presented.

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Quantitative mechanics of 3D printed nanopillars interacting with bacterial cells

Abstract: One of the methods to create sub-10 nm resolution metal-composed 3D nanopillars is electron beam-induced deposition (EBID). Surface nanotopographies (e.g., nanopillars) could play an important role in the design and...

Cited by 16 publications

References 62 publications

On the Use of Black Ti as a Bone Substituting Biomaterial: Behind the Scenes of Dual‐Functionality

On the Use of Black Ti as a Bone Substituting Biomaterial: Behind the Scenes of Dual‐Functionality

3D printed submicron patterns orchestrate the response of macrophages

Additive Manufacturing of Biomaterials—Design Principles and Their Implementation

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