Quantitative measures of aging in the nematode Caenorhabditis elegans. I. Population and longitudinal studies of two behavioral parameters

Bolanowski, M A; Russell, Richard L.; Jacobson, Lewis A.

doi:10.1016/0047-6374(81)90136-6

Cited by 126 publications

(92 citation statements)

References 16 publications

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“…Although the molecular basis of this accumulation rate shift is not known, it is clear that the accelerated rate of age pigment accumulation is correlated with the period of postreproductive decline. Interestingly, discontinuities in rates of change in locomotory rate and defecation frequency have also been noted around the end of the reproductive phase in other studies (Bolanowski et al, 1981;Huang et al, 2004), indicating that the midlife transition is apparent using multiple aging biomarkers.…”

Section: Age-pigment Accumulation Rate Reveals a Postreproductive Midmentioning

confidence: 91%

In vivospectrofluorimetry reveals endogenous biomarkers that report healthspan and dietary restriction inCaenorhabditis elegans

et al. 2005

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SummaryAutofluorescent lipofuscin and advanced glycation end-products (age pigments) accumulate with age across phyla, yet little is understood about their formation under physiological conditions and their specific contributions to the aging process. We used in vivo spectrofluorimetry to quantitate autofluorescence in wild-type Caenorhabditis elegans and longevity mutants disrupted for distinct aspects of the aging process. In wild-type animals, age pigments increase into adulthood, accumulating slowly during the reproductive phase and more rapidly during the post-reproductive period. As in humans, insulin signaling influences age pigment accumulation -mutations that lower efficacy of insulin signaling and extend lifespan [ daf-2 ( e1370 ) insulin receptor and age-1 ( hx546 ) PI3-kinase] dramatically lower age pigment accumulation; conversely, elimination of the insulin-inhibited DAF-16/ FOXO transcription factor causes a huge increase in age pigment accumulation, supporting that the short-lived daf-16 null mutant is truly progeric. By contrast, mutations that increase mitochondrial reactive oxygen species production do not affect age pigment accumulation, challenging assumptions about the role of oxidative stress in generating these species in vivo . Dietary restriction reduces age pigment levels significantly and is associated with a unique spectral shift that might serve as a rapidly scored reporter of the dietary restricted state. Unexpectedly, genetically identical siblings that age poorly (as judged by decrepit locomotory capacity) have dramatically higher levels of age pigments than their same-aged siblings that appear to have aged more gracefully and move youthfully. Thus, high age pigment levels indicate a physiologically aged state rather than simply marking chronological time, and age pigments are valid reporters of nematode healthspan.

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Section: Age-pigment Accumulation Rate Reveals a Postreproductive Midmentioning

confidence: 91%

In vivospectrofluorimetry reveals endogenous biomarkers that report healthspan and dietary restriction inCaenorhabditis elegans

et al. 2005

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“…Bolanowski et al (22) observed no correlation between the lifespan and the rate of decline in movement wave frequency or the decline of defecation frequency when using a longitudinal analysis of WT hermaphrodites. Hosono et al (31) showed that WT hermaphrodites that display rhythmic sinusoidal body movement have a longer lifespan and a higher pharyngeal pumping rate than similarly aged worms that lack this body movement.…”

Section: Discussionmentioning

confidence: 99%

“…This process has been measured quantitatively by counting backward waves per minute on each day of adulthood (21). A single value for each individual or population that can be used for correlation studies has been derived from such data by calculating the rate of decline of body movement and͞or the x-intercept of this line (3,22). The decline of body movement has also been measured by classifying worms into three groups based on qualitative descriptions of body movement (24,31).…”

Section: Quantitative Measurements Of Age-related Changes Of Physiolomentioning

confidence: 99%

“…Several age-related changes have been identified and measured in C. elegans, including declines in physiological processes such as body movement (3,21,22), pharyngeal pumping (21), egg-laying (3,21), and defecation frequency (21,22); changes in appearance such as loss of tissue integrity (23,24), altered yolk protein distribution (24), reduced sperm number (25), increased body size (21), and accumulation of fluorescent material (26); and changes in biochemical properties such as DNA integrity (27). Very little is known about how these changes are related to each other and to lifespan.…”

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confidence: 99%

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Measurements of age-related changes of physiological processes that predict lifespan of Caenorhabditis elegans

Huang

Xiong²,

Kornfeld³

2004

Proc. Natl. Acad. Sci. U.S.A.

481

547

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Aging is characterized by progressive, degenerative changes in many tissues. To elucidate the relationships among degenerative changes in Caenorhabditis elegans, we developed methods to measure age-related changes quantitatively and analyzed correlations among these changes by using a longitudinal study. The age-related declines of pharyngeal pumping and body movement were positively correlated with each other and lifespan. These findings suggest that the declines of pharyngeal pumping and body movement cause a decline in survival probability or that a shared regulatory system mediates the declines in pharyngeal pumping, body movement, and survival probability. Furthermore, measurements of these processes can be used to predict lifespan and detect premature aging. The declines of physiological processes were measured in daf-2, age-1, daf-16, eat-2, and clk-1 mutants that have altered lifespans. Each mutant strain displayed changes in one or more age-related declines, but the correlations among age-related changes were similar to WT. These measurements were used to generate a system of four stages that describes the aging process and is useful for the analysis of genetic and environmental effects on aging.T he identification and measurement of age-related changes, or markers of aging, is a major element of aging research. It is necessary because an understanding of the aging process requires a description of the specific changes that occur. Furthermore, many of the hypotheses about the cause of aging are based on the identification of an age-related change. Because age-related changes are widespread, it is particularly important to determine the relationships among these changes and identify age-related changes that affect lifespan. Three main experimental approaches can be used to investigate these relationships. Longitudinal studies that include serial measurements of the same individual can identify correlations among age-related changes that are measured at different times in the lifespan (1, 2). Cross-sectional studies that include one evaluation of each individual can identify correlations among age-related changes that are measured at the same time in the lifespan. Both longitudinal and cross-sectional data can be used to analyze correlations by comparing two or more populations of animals that have different aging properties as a result of genetic or environmental factors (3, 4). Longitudinal studies provide the most information about the relationships among changes that occur at different times in the lifespan, but longitudinal studies have not been performed frequently because they require substantial time and effort. Here we used longitudinal studies to analyze the relationships among age-related declines of physiological processes and lifespan of the nematode Caenorhabditis elegans.C. elegans is an important model organism for aging research, because it has a short lifespan, and many mutations have been identified that extend the adult lifespan (reviewed by Kenyon in ref. 5). The most extensively char...

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“…FA declines the pharyngeal pumping rate of C. elegans Evaluation of pharynx pumping rate is one measure of aging in C. elegans (Bolanowski et al 1981;Huang et al 2004), and it has been observed that a decline in pumping rate can induce dietary restriction (DR)-like effects in C. elegans (Huang et al 2004). We tested whether FA has any effect on the pharyngeal pumping rates of C. elegans and found that FA treatment significantly decreases the pumping rates of adult day 2, day 5, and day 10 worms compared to control group in a dose-dependent manner (Fig.…”

Section: Fa Extends the Life Span Of Wild-type C Elegansmentioning

confidence: 99%

Folic acid supplementation at lower doses increases oxidative stress resistance and longevity in Caenorhabditis elegans

Rathor

Akhoon

Pandey

et al. 2015

AGE

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Folic acid (FA) is an essential nutrient that the human body needs but cannot be synthesized on its own. Fortified foods and plant food sources such as green leafy vegetables, beans, fruits, and juices are good sources of FA to meet the daily requirements of the body. The aim was to evaluate the effect of dietary FA levels on the longevity of well-known experimental aging model Caenorhabditis elegans. Here, we show for first time that FA extends organism life span and causes a delay in aging. We observed that FA inhibits mechanistic target of rapamycin (mTOR) and insulin/ insulin growth factor 1 (IGF-1) signaling pathways to control both oxidative stress levels and life span. The expression levels of stress-and life span-relevant gerontogenes, viz. daf-16, skn-1, and sir. 2.1, and oxidative enzymes, such as glutathione S-transferase 4 (GST-4) and superoxide dismutase 3 (SOD-3), were also found to be highly enhanced to attenuate the intracellular reactive oxygen species (ROS) damage and to delay the aging process. Our study promotes the use of FA to mitigate abiotic stresses and other aging-related ailments.

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Quantitative measures of aging in the nematode Caenorhabditis elegans. I. Population and longitudinal studies of two behavioral parameters

Cited by 126 publications

References 16 publications

In vivospectrofluorimetry reveals endogenous biomarkers that report healthspan and dietary restriction inCaenorhabditis elegans

In vivospectrofluorimetry reveals endogenous biomarkers that report healthspan and dietary restriction inCaenorhabditis elegans

Measurements of age-related changes of physiological processes that predict lifespan of Caenorhabditis elegans

Folic acid supplementation at lower doses increases oxidative stress resistance and longevity in Caenorhabditis elegans

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