Quantitative Measurement of Superoxide Generation Using the Spin Trap 5-(Diethoxyphosphoryl)-5-methyl- 1-pyrroline-N-oxide

Roubaud, Valérie; Sankarapandi, Sornampillai; Kuppusamy, Periannan; Tordo, Paul; Zweíer, Jay L.

doi:10.1006/abio.1997.2067

Cited by 171 publications

(139 citation statements)

References 30 publications

Supporting

Mentioning

126

Contrasting

Unclassified

Order By: Relevance

“…generation occurred. The DMPO-OOH adduct has a short half of only 45 s (20,21), so the lack of decay indicates that superoxide production continues for more than 30 min. The time course of O 2 .…”

Section: Resultsmentioning

confidence: 99%

Superoxide Generation from Endothelial Nitric-oxide Synthase

Xia

Tsai²,

Berka³

et al. 1998

Journal of Biological Chemistry

Self Cite

643

203

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Superoxide Generation from Endothelial Nitric-oxide Synthase

Xia

Tsai²,

Berka³

et al. 1998

Journal of Biological Chemistry

Self Cite

643

203

View full text Add to dashboard Cite

show abstract

“…The generation of O # d − by illuminated riboflavin is well documented [20,32]. Indeed, riboflavin is used to produce O # d − for determining the efficiency of O # d − scavengers [33]. However O # d − may not be the only species involved in riboflavin photosensitization.…”

Section: Discussionmentioning

confidence: 99%

Superoxide-dependent consumption of nitric oxide in biological media may confound in vitro experiments

Keynes

Griffiths

Garthwaite

2003

Biochemical Journal

View full text Add to dashboard Cite

NO functions ubiquitously as a biological messenger but has also been implicated in various pathologies, a role supported by many reports that exogenous or endogenous NO can kill cells in tissue culture. In the course of experiments aimed at examining the toxicity of exogenous NO towards cultured cells, we found that most of the NO delivered using a NONOate (diazeniumdiolate) donor was removed by reaction with the tissue-culture medium. Two NO-consuming ingredients were identified : Hepes buffer and, under laboratory lighting, the vitamin riboflavin. In each case, the loss of NO was reversed by the addition of superoxide dismutase. The effect of Hepes was observed over a range of NONOate concentrations (producing up to 1 µM NO). Furthermore, from measurements of soluble guanylate cyclase activity, Hepes-dependent NO consumption remained significant at the low nanomolar NO concentrations relevant to physiological NO

show abstract

“…3) DEPMPO, which generated a more stable adduct of superoxide anion radicals, 21,22) was used as the agent in an attempt to detect the superoxide adduct from the extract by the spin-trapping technique. The overlapped signals for DEPMPO-OOH and DEPMPO-OH 23,24) were detected with an acquisition time of 30 min (A in Fig.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Superoxide Anion Radicals Generated from an Aqueous Extract of Particulate Phase Cigarette Smoke by Electron Spin Resonance Using 5,5-Dimethyl-1-pyrroline-N-oxide

Takanami

Nakayama

2011

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

The reactive oxygen species generated by an aqueous extract of the particulate phase of cigarette smoke were evaluated by an electron spin resonance (ESR) analysis, using spin-trapping agents, and by comparing with model reaction systems. The ESR signals of DMPO-OH were detected from the extract by using 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). These signals were eliminated by adding superoxide dismutase, but hardly by catalase. These responses of the ESR signals to the scavengers were similar to those of a hypoxanthinexanthine oxidase system. The results indicate that the signals of DMPO-OH from the extract were derived from a reaction product of DMPO with superoxide anion radicals and clarify the mechanism by which the extract generated superoxide anion radicals.

show abstract

Quantitative Measurement of Superoxide Generation Using the Spin Trap 5-(Diethoxyphosphoryl)-5-methyl- 1-pyrroline-N-oxide

Cited by 171 publications

References 30 publications

Superoxide Generation from Endothelial Nitric-oxide Synthase

Superoxide Generation from Endothelial Nitric-oxide Synthase

Superoxide-dependent consumption of nitric oxide in biological media may confound in vitro experiments

Evaluation of Superoxide Anion Radicals Generated from an Aqueous Extract of Particulate Phase Cigarette Smoke by Electron Spin Resonance Using 5,5-Dimethyl-1-pyrroline-N-oxide

Contact Info

Product

Resources

About