Quantitative measurement of metal chelation by fourier transform infrared spectroscopy

Miller, Monika E.; McKinnon, Lani P.; Walker, Edward B.

doi:10.1016/j.ancr.2015.10.002

Cited by 9 publications

(3 citation statements)

References 13 publications

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“…4. A similar shift effect was observed for the other metal citrates (Miller et al, 2015, Jumanath et al, 2019, Gajbhiye et al, 1996. The difference between the asymmetrical (1658 and 1605 cm -1 ) and symmetrical (1420 cm -1 and 1397cm -1 ) vibrations indicates that one citrate ion coordinates one metal atom (Raju et al, 2017).…”

Section: Ftir Analysissupporting

confidence: 73%

Synthesis of citrates of selected lanthanides (Er, Ho and Lu)

Janusz¹,

Pikus²,

Skwarek³

et al. 2020

Physicochem. Probl. Miner. Process.

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Holmium, erbium and lutetium citrates can be synthesized by transforming freshly precipitated holmium, erbium and lutetium hydroxides in the citric acid solution under the hydrothermal conditions (above 100°C) after 3 days of reaction. In this paper in order to determine the synthesis conditions, the hydroxide and citrate stability areas of selected lanthanides were compared. The studies of the structure of the obtained holmium citrate, erbium and lutetium samples showed that these compounds crystallize in a monoclinic system. The crystallite sizes determined by the Halder-Wagner method were 67.8 ± 8.0nm, 103.7 ± 4.5nm and 68.1 ± 4.2nm, respectively for the holmium citrate, erbium citrate and lutetium citrate samples. The shifts of the hydroxyl and carboxyl groups of citric acid in the FTIR spectrum indicate the interactions of both groups with holmium, erbium and lutetium cations. Then the obtained samples were subjected to the analysis of composition, and their particle size distribution was determined.

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Section: Ftir Analysissupporting

confidence: 73%

Synthesis of citrates of selected lanthanides (Er, Ho and Lu)

Janusz¹,

Pikus²,

Skwarek³

et al. 2020

Physicochem. Probl. Miner. Process.

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“…Some organic acids have been reported to bind to metals (Liu and Lin, 2013; Miller et al, 2015; do Nascimento et al, 2006). In response to the double deficiency in Cu and Fe, the WT and fum2 lines displayed similar contents of malate, whereas citrate increased about three‐fold in all three lines (Figure S2b).…”

Section: Resultsmentioning

confidence: 99%

Inactivation of cytosolic FUMARASE2 enhances growth and photosynthesis under simultaneous copper and iron deprivation in Arabidopsis

et al. 2021

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Copper (Cu) and iron (Fe) are essential for plant growth and are often in short supply under natural conditions. Molecular responses to simultaneous lack of both metals (-Cu-Fe) differ from those seen in the absence of either alone. Metabolome profiling of plant leaves previously revealed that fumarate levels fall under -Cu-Fe conditions. We employed lines lacking cytosolic FUMARASE2 (FUM2) activity to study the impact of constitutive suppression of cytosolic fumarate synthesis on plant growth under Cu and/or Fe deficiency. In fum2 mutants, photosynthesis and growth were less impaired under -Cu-Fe conditions than in wild-type (WT) seedlings. In particular, levels of photosynthetic proteins, chloroplast ultrastructure, amino acid profiles and redox state were less perturbed by simultaneous Cu-Fe deficiency in lines that cannot produce fumarate in the cytosol. Although cytosolic fumarate has been reported to promote acclimation of photosynthesis to low temperatures when metal supplies are adequate, the photosynthetic efficiency of fum2 lines grown under Cu-Fe deficiency in the cold was higher than in WT. Uptake and contents of Cu and Fe are similar in WT and fum2 plants under control and -Cu-Fe conditions, and lack of FUM2 does not alter the ability to sense metal deficiency, as indicated by marker gene expression. Collectively, we propose that reduced levels of cytosolic fumarate synthesis ultimately increase the availability of Fe for incorporation into metalloproteins.

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“…The solid proof that these combinations are truly chelates is still missing and most publications suppose the obtained products to be chelates due to the well-known fact of mineral chelation. Indeed, these products might be physical mixtures of reactants or unreacted dry blends [4].…”

Section: Introductionmentioning

confidence: 99%

Are Citric Acid-Iron II Complexes True Chelates or Just Physical Mixtures and How to Prove This?

Mattar

Haddarah

Haddad

et al. 2023

Foods

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Although mineral chelates are widely produced to be used as food fortifiers, the proof that these complexes are chelates is still missing. In our present work, iron II complexes using citric acid in different ratios are produced, and the occurrence of chelation is investigated along with its behavior according to a molar ratio between the ligand and the mineral. High performance liquid chromatography (HPLC), flame atomic absorption spectroscopy (FAAS), ultraviolet-visible spectroscopy (UV-Vis), Fourier-transform infrared (FTIR), and near infrared spectroscopy (NIR) were used for a non-structural characterization of these complexes. In contrast to published work, our findings show that the chelation of citric acid is achieved in the liquid form and at a low pH and that the molar ratio is very important in setting the direction of the reaction, either toward chelation or dimer formation. The ratio citric acid:iron 1:4 seems to be the most convenient ratio in which no free citric acid remains in the solution, while the 1:3 ratio behaves differently, requiring further investigations by such techniques as extended X-ray absorption fine structure spectroscopy (EXAFS), among others, in order to deeply identify the structural organization occurring in this ratio. NIR, extensively used in industries, proved to be very useful in the demonstration and characterization of chelates. These findings are particularly advantageous for pharmaceutical and food industries in offering an innovative competent fortifying agent to be used in combatting iron deficiency.

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Quantitative measurement of metal chelation by fourier transform infrared spectroscopy

Cited by 9 publications

References 13 publications

Synthesis of citrates of selected lanthanides (Er, Ho and Lu)

Synthesis of citrates of selected lanthanides (Er, Ho and Lu)

Inactivation of cytosolic FUMARASE2 enhances growth and photosynthesis under simultaneous copper and iron deprivation in Arabidopsis

Are Citric Acid-Iron II Complexes True Chelates or Just Physical Mixtures and How to Prove This?

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