Quantitative measurement of damage caused by 1064-nm wavelength optical trapping of Escherichia coli cells using on-chip single cell cultivation system

Ayano, Satoru; Wakamoto, Yuichi; Yamashita, Shoko; Yasuda, Kenji

doi:10.1016/j.bbrc.2006.09.115

Cited by 57 publications

(47 citation statements)

References 20 publications

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“…3 and 4͒. These results correlate well with data from similar experiments done on other microorganisms, 8,11 and they stress the fact that using lasers with a wavelength of 1070 nm to trap and manipulate S. cer- evisiae cells over long time periods should be approached with great caution.…”

Section: Discussionsupporting

confidence: 86%

“…Recently, using 1064 nm, it was shown that E. coli cell damage was linearly dependent on the total dose received and that the growth rate was adversely affected at a dose of 0.5 J, while cell division ability was affected 11 at an even lower dose of 0.35 J. However, Mohanty et al found DNA damage in human lymphoblasts to be dependent not only on total dose but also on peak power.…”

Section: Introductionmentioning

confidence: 99%

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Effect of long- and short-term exposure to laser light at <inline-formula><math display="inline" overflow="scroll"><mrow><mn>1070</mn><mspace width="0.3em" /><mi>nm</mi></mrow></math></inline-formula> on growth of <italic>Saccharomyces cerevisiae</italic>

et al. 2010

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Abstract. The effect of a 1070-nm continuous and pulsed wave ytterbium fiber laser on the growth of Saccharomyces cerevisiae single cells is investigated over a time span of 4 to 5 h. The cells are subjected to optical traps consisting of two counterpropagating plane wave beams with a uniform flux along the x, y axis. Even at the lowest continuous power investigated-i.e., 0.7 mW-the growth of S. cerevisiae cell clusters is markedly inhibited. The minimum power required to successfully trap single S. cerevisiae cells in three dimensions is estimated to be 3.5 mW. No threshold power for the photodamage, but instead a continuous response to the increased accumulated dose is found in the regime investigated from 0.7 to 2.6 mW. Furthermore, by keeping the delivered dose constant and varying the exposure time and power-i.e. pulsing-we find that the growth of S. cerevisiae cells is increasingly inhibited with increasing power. These results indicate that growth of S. cerevisiae is dependent on both the power as well as the accumulated dose at 1070 nm.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Effect of long- and short-term exposure to laser light at <inline-formula><math display="inline" overflow="scroll"><mrow><mn>1070</mn><mspace width="0.3em" /><mi>nm</mi></mrow></math></inline-formula> on growth of <italic>Saccharomyces cerevisiae</italic>

et al. 2010

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“…21.6 J for one hour exposure in their study) [19]. Ayano showed that cell damage to E.coli was linearly dependent on the total dose received and found that cell division ability was affected at a dose larger than 0.35 J [23]. Furthermore, Aabo's study indicates that the photodamage to S. cerevisiae is dependent on both the laser power and the accumulated dose [24].…”

Section: Introductionmentioning

confidence: 99%

Optical micromanipulation

2008

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“…31 These authors observed that continuous exposure of cells to 3 mW of laser illumination for 3 hr resulted in complete stoppage of cell growth. Indeed, light exposure to living cells during these experiments is considerable.…”

Section: Introductionmentioning

confidence: 99%

Automated single cell microbioreactor for monitoring intracellular dynamics and cell growth in free solution

et al. 2014

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We report an automated microfluidic-based platform for single cell analysis that allows for cell culture in free solution with the ability to control the cell growth environment. Using this approach, cells are confined by the sole action of gentle fluid flow, thereby enabling non-perturbative analysis of cell growth away from solid boundaries. In addition, the single cell microbioreactor allows for precise and time-dependent control over cell culture media, with the combined ability to observe the dynamics of non-adherent cells over long time scales. As a proof-of-principle demonstration, we used the platform to observe dynamic cell growth, gene expression, and intracellular diffusion of repressor proteins while precisely tuning the cell growth environment. Overall, this microfluidic approach enables the direct observation of cellular dynamics with exquisite control over environmental conditions, which will be useful for quantifying the behaviour of single cells in well-defined media.

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Quantitative measurement of damage caused by 1064-nm wavelength optical trapping of Escherichia coli cells using on-chip single cell cultivation system

Cited by 57 publications

References 20 publications

Effect of long- and short-term exposure to laser light at <inline-formula><math display="inline" overflow="scroll"><mrow><mn>1070</mn><mspace width="0.3em" /><mi>nm</mi></mrow></math></inline-formula> on growth of <italic>Saccharomyces cerevisiae</italic>

Effect of long- and short-term exposure to laser light at <inline-formula><math display="inline" overflow="scroll"><mrow><mn>1070</mn><mspace width="0.3em" /><mi>nm</mi></mrow></math></inline-formula> on growth of <italic>Saccharomyces cerevisiae</italic>

Optical micromanipulation

Automated single cell microbioreactor for monitoring intracellular dynamics and cell growth in free solution

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