Abstract:Common transparent polymer films, such as cellophane and household tape, are frequently used as examples of birefringent materials in textbooks and classroom demonstrations. Qualitatively, birefringence is often demonstrated by layering such films between crossed-polarizers. In this work, we describe an inexpensive experimental setup for the quantitative measurement of birefringence in common household films, suitable for senior high school or undergraduate labs. Whereas traditional approaches for polarization… Show more
“…Finally, we estimated the film birefringence by accounting for the proportion of backing material (65.5%) to the whole layer thickness. The estimated birefringence was 0.0012, within the literature value range (0.0007-0.0022) [55,56], while it can vary significantly by environmental factors such as stress and temperature.…”
Clinicians are unable to detect glaucoma until substantial loss or dysfunction of retinal ganglion cells occurs. To this end, novel measures are needed. We have developed an optical imaging solution based on adaptive optics optical coherence tomography (AO-OCT) to discern key clinical features of glaucoma and other neurodegenerative diseases at the cellular scale in the living eye. Here, we test the feasibility of measuring AO-OCT-based reflectance, retardance, optic axis orientation, and angiogram at specifically targeted locations in the living human retina and optic nerve head. Multifunctional imaging, combined with focus stacking and global image registration algorithms, allows us to visualize cellular details of retinal nerve fiber bundles, ganglion cell layer somas, glial septa, capillaries, and connective tissues. These are key histologic features of neurodegenerative diseases, including glaucoma, that are now able to examine in vivo with excellent repeatability and reproducibility. Incorporating this noninvasive cellular-scale imaging with objective measurements will significantly enhance existing clinical assessments, which is pivotal in facilitating the early detection of eye disease and understanding the mechanisms of neurodegeneration.
“…Finally, we estimated the film birefringence by accounting for the proportion of backing material (65.5%) to the whole layer thickness. The estimated birefringence was 0.0012, within the literature value range (0.0007-0.0022) [55,56], while it can vary significantly by environmental factors such as stress and temperature.…”
Clinicians are unable to detect glaucoma until substantial loss or dysfunction of retinal ganglion cells occurs. To this end, novel measures are needed. We have developed an optical imaging solution based on adaptive optics optical coherence tomography (AO-OCT) to discern key clinical features of glaucoma and other neurodegenerative diseases at the cellular scale in the living eye. Here, we test the feasibility of measuring AO-OCT-based reflectance, retardance, optic axis orientation, and angiogram at specifically targeted locations in the living human retina and optic nerve head. Multifunctional imaging, combined with focus stacking and global image registration algorithms, allows us to visualize cellular details of retinal nerve fiber bundles, ganglion cell layer somas, glial septa, capillaries, and connective tissues. These are key histologic features of neurodegenerative diseases, including glaucoma, that are now able to examine in vivo with excellent repeatability and reproducibility. Incorporating this noninvasive cellular-scale imaging with objective measurements will significantly enhance existing clinical assessments, which is pivotal in facilitating the early detection of eye disease and understanding the mechanisms of neurodegeneration.
“…The birefringence of standard adhesive tapes was evaluated in a recent study 27 . Here, a multi-layered tape made of polyethylene and adhesive (each layer ∼0.05mm thick) was used to simulate layered structure of biological tissues.…”
Section: Resultsmentioning
confidence: 99%
“…The birefringence of standard adhesive tapes was evaluated in a recent study. 27 Here, a multi-layered tape made of polyethylene and adhesive (each layer thick) was used to simulate layered structure of biological tissues. The laminated structure will deform under compression, and we used this multi-layered phantom to simulate the impact of structural modifications on polarization [ Fig.…”
Diabetes can lead to the glycation of proteins and dysfunction of skin collagen. Skin lesions are a prevalent clinical symptom of diabetes mellitus (DM). Early diagnosis and assessing the efficacy of treatment for DM are crucial for patient health management. However, performing a non-invasive skin assessment in the early stages of DM is challenging.Aim: By using the polarization-sensitive optical coherent tomography (PS-OCT) imaging technique, it is possible to noninvasively assess the skin changes caused by diabetes.Approach: The PS-OCT was used to monitor the polarization characteristics of mouse skin at different stages of diabetes.Results: Based on a multi-layered adhesive tape model, we found that the polarization characteristics (retardation, optic axis, and polarization uniformity) were sensitive to the microstructure changes in the samples. Through this method, we observed significant changes in the polarization states of the skin as diabetes progressed. This was in line with the detected microstructure changes in skin collagen fibers using scanning electron microscopy.Conclusions: This study presents a highly useful approach for non-invasive skin assessment of diabetes.
“…Slepkov published his findings last July in the American Journal of Physics ( 3 ). His paper isn’t the first to connect properties such as thickness, layering, and crystal choice to the colors they produce when illuminated.…”
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