2021
DOI: 10.1007/s00216-021-03210-0
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Quantitative mass spectrometry imaging of drugs and metabolites: a multiplatform comparison

Abstract: Mass spectrometry imaging (MSI) provides insight into the molecular distribution of a broad range of compounds and, therefore, is frequently applied in the pharmaceutical industry. Pharmacokinetic and toxicological studies deploy MSI to localize potential drugs and their metabolites in biological tissues but currently require other analytical tools to quantify these pharmaceutical compounds in the same tissues. Quantitative mass spectrometry imaging (Q-MSI) is a field with challenges due to the high biological… Show more

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Cited by 34 publications
(26 citation statements)
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References 49 publications
(61 reference statements)
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“…The increased sensitivity of the DESI-TQ setup for olanzapine and its metabolites stand in contrast to previous work published by Lamont et al who evaluated the LOD for two drug candidates on a similar instrument setup using liver tissue mimetics [ 18 ]. The use of such mimetic-based calibration standards is required for the reported quantitative MSI workflow and does enable to approximate the sensitivity of the different instrument setups delivering a quantitative readout.…”
Section: Resultsmentioning
confidence: 71%
“…The increased sensitivity of the DESI-TQ setup for olanzapine and its metabolites stand in contrast to previous work published by Lamont et al who evaluated the LOD for two drug candidates on a similar instrument setup using liver tissue mimetics [ 18 ]. The use of such mimetic-based calibration standards is required for the reported quantitative MSI workflow and does enable to approximate the sensitivity of the different instrument setups delivering a quantitative readout.…”
Section: Resultsmentioning
confidence: 71%
“…Combinations of multiplexed IHC with targeted proteomic access could greatly expand our knowledge of cell subtypes in cancer [61]. Current quantitative approaches in imaging mass spectrometry include on-tissue spotting of standards, internal standards and the mimetic models [62][63][64][65][66]. An additional advancement to this method could be made by utilizing synthesized peptide standards of the ECM components to obtain quantitative information.…”
Section: Discussionmentioning
confidence: 99%
“…10,11 The repeatability is mainly affected by spot-to-spot random errors for sampling/ionization, tissue thickness or topological height differences within a tissue or between tissues, and also MS detection modes. 45,47,55 They were discussed in details in the following based on different MSI techniques.…”
Section: Repeatability Of Surface Sampling/ionizationmentioning
confidence: 99%