Quantitative Mass Spectrometry-Based Proteomic Profiling for Precision Medicine in Prostate Cancer

Abstract

Prostate cancer (PCa) is one of the most frequently diagnosed cancer among men in the western societies. Many PCa patients bear tumors that will not threat their lives if left untreated or if treatment is delayed. Our inability for early identification of these patients has resulted in massive overtreatment. Therefore, there is a great need of finding biomarkers for patient stratification according to prognostic risk; as well as there is a need for novel targets that can allow the development of effective treatments for patients that progress to castration-resistant PCa. Most biomarkers in cancer are proteins, including the widely-used prostate-specific antigen (PSA). Recent developments in mass spectrometry allow the identification and quantification of thousands of proteins and posttranslational modifications from small amounts of biological material, including formalin-fixed paraffin-embedded tissues, and biological fluids. Novel diagnostic and prognostic biomarkers have been identified in tissue, blood, urine, and seminal plasma of PCa patients, and new insights in the ethology and progression of this disease have been achieved using this technology. In this review, we summarize these findings and discuss the potential of this technology to pave the way toward the clinical implementation of precision medicine in PCa.

Characterization of HMGB1/2 Interactome in Prostate Cancer by Yeast Two Hybrid Approach: Potential Pathobiological Implications

“…Proteomic studies in relation to PCa have been reported [20,21,22], with interactome strategies being outstanding in recent developments [23,24,25]. The purpose of our study was to analyze proteins interacting with HMGB1 and HMGB2 by the yeast 2-hybrid approach (Y2H), using HMGB1 and HMGB2 baits.…”

Absolute Quantification of Apolipoproteins Following Treatment with Omega-3 Carboxylic Acids and Fenofibrate Using a High Precision Stable Isotope-labeled Recombinant Protein Fragments Based SRM Assay

“…The transition toward personalized medicine raises the importance of accurate and precise absolute quantification of protein biomarkers to identify patients who can benefit from targeted therapies (1, 2). Immunoassays have traditionally been the technology of choice for quantification of protein biomarkers.…”

Clinical significance of serum PSA in breast cancer patients

“…The evaluation of PSA by IHC in breast cancer has not been fully established and positive rates of PSA vary greatly depending on reports [5, 38, 39]. It may be useful to verify by combining quantitative methods such as time resolved immunofluorometric assay or Mass Spectrometry-Based Proteomic Profiling [40, 41]. In the present study, we found a negative correlation between sPSA and Ki67 in post-menopausal MBC (Table 3, Fig.…”