Quantitative mass spectrometric analysis to unravel glycoproteomic signature of follicular fluid in women with polycystic ovary syndrome

Yelamanchi, Soujanya D.; Kumar, Manish; Hinduja, Indira; Prasad, T. S. Keshava; Gowda, Harsha; Patil, Krutika

doi:10.1371/journal.pone.0214742

Cited by 21 publications

(11 citation statements)

References 48 publications

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“…Specific FRF protein components putatively involved in the interactions with seminal fluid have been identified by proteomic analysis [81,82], or by comparing their sequence evolution rate [83,84]. For example, mammalian oviductal glycoproteins show signs of positive Darwinian selection [85], suggesting that they may play a role in FRF-seminal fluid interaction [86].…”

Section: Female Reproductive Fluid-ejaculate Interactions: Mechanismsmentioning

confidence: 99%

The role of female reproductive fluid in sperm competition

Gasparini

Pilastro

Evans

2020

Phil. Trans. R. Soc. B

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The role of non-gametic components of the ejaculate (seminal fluid) in fertility and sperm competitiveness is now well established. Surprisingly, however, we know far less about female reproductive fluid (FRF) in the context of sexual selection, and insights into male–FRF interactions in the context of sperm competition have only recently emerged. Despite this limited knowledge, evidence from taxonomically diverse species has revealed insights into the effects of FRF on sperm traits that have previously been implicated in studies of sperm competition. Specifically, through the differential effects of FRF on a range of sperm traits, including chemoattraction and alterations in sperm velocity, FRF has been shown to exert positive phenotypic effects on the sperm of males that are preferred as mating partners, or those from the most compatible or genetically diverse males. Despite these tantalizing insights into the putative sexually selected functions of FRF, we largely lack a mechanistic understanding of these processes. Taken together, the evidence presented here highlights the likely ubiquity of FRF-regulated biases in fertilization success across a diverse range of taxa, thus potentially elevating the importance of FRF to other non-gametic components that have so far been studied largely in males. This article is part of the theme issue ‘Fifty years of sperm competition'.

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Section: Female Reproductive Fluid-ejaculate Interactions: Mechanismsmentioning

confidence: 99%

The role of female reproductive fluid in sperm competition

Gasparini

Pilastro

Evans

2020

Phil. Trans. R. Soc. B

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“…One of the five major classes of glycans, N-glycans are components of N-glycoproteins that have pivotal roles in cell migration, angiogenesis, and cell–cell adhesion ( Kuo et al, 2011 ). It has also been indicated that they are involved in the regulation of oocyte maturation and ovulation by regulating fibrinolysis, blood coagulation, angiogenesis, extracellular matrix stabilization, cholesterol homeostasis, inflammation, and complement and antioxidant pathways in stimulated IVF/ICSI cycles ( Lim et al, 2017 ; Patil et al, 2019 ). Additionally, heparan sulfate proteoglycans contribute to the patterning of oocyte signaling and cumulus cell function ( Watson et al, 2012 ).…”

Section: Resultsmentioning

confidence: 99%

Reporting on the Role of miRNAs and Affected Pathways on the Molecular Backbone of Ovarian Insufficiency: A Systematic Review and Critical Analysis Mapping of Future Research

Rapani

Nikiforaki

Karagkouni

et al. 2021

Front. Cell Dev. Biol.

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Ovarian insufficiency is identified as a perplexing entity in the long list of pathologies impairing fertility dynamics. The three distinct classifications of ovarian insufficiency are poor ovarian response, premature ovarian insufficiency/failure, and advanced maternal age, sharing the common denominator of deteriorated ovarian reserve. Despite efforts to define clear lines among the three, the vast heterogeneity and overlap of clinical characteristics renders their diagnosis and management challenging. Lack of a consensus has prompted an empirically based management coupled by uncertainty from the clinicians’ perspective. Profiling of patients in the era of precision medicine seems to be the way forward, while the necessity for a novel approach is underlined. Implicating miRNAs in the quest for patient profiling is promising in light of their fundamental role in cellular and gene expression regulation. To this end, the current study sets out to explore and compare the three pathophysiologies—from a molecular point of view—in order to enable profiling of patients in the context of in vitro fertilization treatment and enrich the data required to practice individualized medicine. Following a systematic investigation of literature, data referring to miRNAs were collected for each patient category based on five included studies. miRNA–target pairs were retrieved from the DIANA-TarBase repository and microT-CDS. Gene and miRNA annotations were derived from Ensembl and miRbase. A subsequent gene-set enrichment analysis of miRNA targets was performed for each category separately. A literature review on the most crucial of the detected pathways was performed to reveal their relevance to fertility deterioration. Results supported that all three pathophysiologies share a common ground regarding the affected pathways, naturally attributed to the common denominator of ovarian insufficiency. As evidenced, miRNAs could be employed to explore the fine lines and diverse nature of pathophysiology since they constitute invaluable biomarkers. Interestingly, it is the differentiation through miRNAs and not through the molecular affected pathways that corresponds to the three distinctive categories. Alarming discrepancies among publications were revealed, pertaining to employment of empirical and arbitrary criteria in categorizing the patients. Following bioinformatic analysis, the final step of the current study consisted of a critical analysis of the molecular data sourced, providing a clear and unique insight into the physiological mechanisms involved. It is our intention to contribute to mapping future research dedicated to ovarian insufficiency and to help researchers navigate the overwhelming information published in molecular studies.

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“…On the day of ovum pick up (d-OPU), blood was collected from all participants for carrying out biochemical and hormonal assays. On the same day macroscopically clear follicular fluid was collected, processed as described previously [34]. Serum and follicular fluid collected on d-OPU were assayed for estradiol (E 2 ), progesterone (P 4 ), total testosterone (TT) and SHBG by electro-chemiluminescence technology using Roche e411 automated analyser (Roche, Basel, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

Ovarian Granulosa Cells From Women With Pcos Express Low Levels of Sars-Cov-2 Receptors and Co-Factors

Naigaonkar

Patil

Joseph

et al. 2021

Preprint

Self Cite

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Purpose: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection is global pandemic with more than 3 million deaths so far. Female reproductive tract organs express coronavirus-associated receptors and factors (SCARFs); suggesting they may be susceptible to SARS-CoV-2 infection however the susceptibility of ovary/follicle/oocyte to the same is still elusive. Co-morbidities like obesity, type-2 diabetes mellitus, cardiovascular disease etc. increase the risk of SARS-CoV-2 infection. These features are common in women with polycystic ovary syndrome (PCOS), warranting further scope to study SCARFs expression in ovary of these women. Materials and methods: SCARFs expression in ovary and ovarian tissues of women with PCOS and healthy women was explored by analyzing publically available microarray datasets. Transcript expression of SCARFs were investigated in mural and cumulus granulosa cells (MGCs and CGCs) from control and PCOS women undergoing in vitro fertilization (IVF). Results: Microarray data revealed that ovary expresses all genes necessary for SARS-CoV-2 infection. PCOS women mostly showed down-regulated/unchanged levels of SCARFs. MGCs and CGCs from PCOS women showed lower expression of receptors ACE2, BSG and DPP4 and protease CTSB than in controls. MGCs showed lower expression of protease CTSL in PCOS than in controls. Expression of TMPRSS2 was not detected in both cell types. Conclusions: Human ovarian follicle may be susceptible to SARS-CoV-2 infection. Lower expression of SCARFs in PCOS indicate that the risk of SARS-CoV-2 infection to the ovary may be lesser in these women than controls. This knowledge may help in safe practices at IVF settings in the current pandemic. Keywords: SARS-CoV-2, COVID-19, Ovarian granulosa cells, Oocyte, PCOS, IVF

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Quantitative mass spectrometric analysis to unravel glycoproteomic signature of follicular fluid in women with polycystic ovary syndrome

Cited by 21 publications

References 48 publications

The role of female reproductive fluid in sperm competition

The role of female reproductive fluid in sperm competition

Reporting on the Role of miRNAs and Affected Pathways on the Molecular Backbone of Ovarian Insufficiency: A Systematic Review and Critical Analysis Mapping of Future Research

Ovarian Granulosa Cells From Women With Pcos Express Low Levels of Sars-Cov-2 Receptors and Co-Factors

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