Quantitative Mapping of the Orientation of Fibroin β-Sheets in B. mori Cocoon Fibers by Scanning Transmission X-ray Microscopy

Cruz, Daniel Hernández; Rousseau, Marie‐Eve; West, Marcia; Pézolet, Michel; Hitchcock, Adam P.

doi:10.1021/bm050943u

Cited by 55 publications

(42 citation statements)

References 33 publications

(99 reference statements)

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“…By contrast, fibers spun naturally by silkworms, which consist of aligned fibroin fibrils coated with sericin, exhibit birefrigent behaviour, with the optical axis aligned along the axis of the fibril, as shown in Supplementary Fig. 2g, and consistent with previous literature reports252627. We then proceeded to probe in this manner the microcapsules generated in this work.…”

Section: Resultssupporting

confidence: 87%

Silk micrococoons for protein stabilisation and molecular encapsulation

et al. 2017

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Naturally spun silks generate fibres with unique properties, including strength, elasticity and biocompatibility. Here we describe a microfluidics-based strategy to spin liquid native silk, obtained directly from the silk gland of Bombyx mori silkworms, into micron-scale capsules with controllable geometry and variable levels of intermolecular β-sheet content in their protein shells. We demonstrate that such micrococoons can store internally the otherwise highly unstable liquid native silk for several months and without apparent effect on its functionality. We further demonstrate that these native silk micrococoons enable the effective encapsulation, storage and release of other aggregation-prone proteins, such as functional antibodies. These results show that native silk micrococoons are capable of preserving the full activity of sensitive cargo proteins that can aggregate and lose function under conditions of bulk storage, and thus represent an attractive class of materials for the storage and release of active biomolecules.

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Section: Resultssupporting

confidence: 87%

Silk micrococoons for protein stabilisation and molecular encapsulation

et al. 2017

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“…As a result, only a few techniques can provide structural information about silk monofilaments, including microscopic methods (atomic force microscopy, [1][2][3][4][5][6] scanning 3,5 and transmission electron microscopy, 7,8 and scanning transmission X-ray microscopy 9,10 ) and synchrotron X-ray diffraction. 11,12 Nevertheless, some of these techniques require a certain degree of treatment of the fiber that might affect its structure and/or they provide no molecular (i.e., spectroscopic) information.…”

Section: Introductionmentioning

confidence: 99%

Structure of silk by raman spectromicroscopy: From the spinning glands to the fibers

et al. 2011

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Raman spectroscopy has long been proved to be a useful tool to study the conformation of protein‐based materials such as silk. Thanks to recent developments, linearly polarized Raman spectromicroscopy has appeared very efficient to characterize the molecular structure of native single silk fibers and spinning dopes because it can provide information relative to the protein secondary structure, molecular orientation, and amino acid composition. This review will describe recent advances in the study of the structure of silk by Raman spectromicroscopy. A particular emphasis is put on the spider dragline and silkworm cocoon threads, other fibers spun by orb‐weaving spiders, the spinning dope contained in their silk glands and the effect of mechanical deformation. Taken together, the results of the literature show that Raman spectromicroscopy is particularly efficient to investigate all aspects of silk structure and production. The data provided can lead to a better understanding of the structure of the silk dope, transformations occurring during the spinning process, and structure and mechanical properties of native fibers. © 2011 Wiley Periodicals, Inc. Biopolymers 97: 322–336, 2012.

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“…28 STXM has only been applied to a limited number of biological or biomedical samples in the past. 21,[28][29][30][31][32][33][34][35] To the best of our knowledge, the interaction of particles in the submicron and nanometer range with human skin samples has not been investigated by STXM. Transmission electron microscopy ͑TEM͒ has already been used to localize the TiO 2 nanoparticles in skin sections, but this technique requires ultrathin skin sections ͑50 nm͒ and staining of the samples.…”

Section: Introductionmentioning

confidence: 99%

Qualitative detection of single submicron and nanoparticles in human skin by scanning transmission x-ray microscopy

et al. 2009

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First results on single particle detection in human skin samples by x-ray microscopy are reported. 94+/-6 and 161+/-13 nm gold core particles with silica shells and 298+/-11 nm silica particles coated with a gold shell on ultramicrotome sections of human skin were determined. The particles were applied on fresh intact skin samples, which were sectioned prior to imaging. After screening the sections by conventional microscopy techniques, defined areas of interest were qualitatively investigated by scanning transmission x-ray microscopy at the Swiss Light Source. In studies on the percutaneous penetration of 161+/-13 nm particles on human skin samples, x-ray microscopy yielded high-resolution images of single particles spreading on the superficial layer of the stratum corneum and on the epithelium in superficial parts of hair follicles. No deeper penetration was observed. The present work using x-ray microscopy provides the unique opportunity to study qualitative penetration processes and membrane-particle interactions on the level of single particles. This goes beyond present approaches using optical microscopy. Further improvement of this approach will allow one to study particles with different physicochemical properties and surface modifications, including responses of the exposed tissue.

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Quantitative Mapping of the Orientation of Fibroin β-Sheets in B. mori Cocoon Fibers by Scanning Transmission X-ray Microscopy

Cited by 55 publications

References 33 publications

Silk micrococoons for protein stabilisation and molecular encapsulation

Silk micrococoons for protein stabilisation and molecular encapsulation

Structure of silk by raman spectromicroscopy: From the spinning glands to the fibers

Qualitative detection of single submicron and nanoparticles in human skin by scanning transmission x-ray microscopy

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