Quantitative liquid chromatographic determination of sanguinarine in cell culture medium and in rat urine and plasma

Hoellinger, H.; Ré, Micheline; Deroussent, Alain; Singh, Ravindra Pratap; Cresteil, Thierry

doi:10.1016/j.jchromb.2003.10.023

Cited by 15 publications

(10 citation statements)

References 17 publications

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“…The molecular ion SA [M + H + ] was measured at m/z 332 and specific products of fragmentation at m/z 317 and 274, the molecular ion DHSA [M + H + ] was measured at m/z 334 and specific products of fragmentation at m/z 318, 260 and 232, and the molecular ion BCA [M + H + ] was measured at m/z 230 and specific products of fragmentation at m/z 202. According to literature, the fragments were in good coincidence with so far published data [9][10][11].…”

Section: Procedures Optimizationsupporting

confidence: 80%

A liquid chromatographic–mass spectrometric evidence of dihydrosanguinarine as a first metabolite of sanguinarine transformation in rat

Psotová

Klejdus

Večeřa

et al. 2006

Journal of Chromatography B

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Section: Procedures Optimizationsupporting

confidence: 80%

A liquid chromatographic–mass spectrometric evidence of dihydrosanguinarine as a first metabolite of sanguinarine transformation in rat

Psotová

Klejdus

Večeřa

et al. 2006

Journal of Chromatography B

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“…5-1): precursor ion m/z 332.09 (M + , calc. for C 20 H 13 NO 4 ), product ion m/z 317.07 [M-CH 3 ] + , 304.10 [M-CO] + , 289.07 [M-CO-CH 3 ] + , 274.08 [M-CO-CH 2 O] + , 246.09 [M-2CO-CH 2 O] + , 216.08 [M-2CO-2CH 2 O] + were in good agreement with the literature [18]. So compound 1 was identified as sanguinarine.…”

Section: Resultssupporting

confidence: 87%

Preparative isolation of alkaloids from Corydalis bungeana Turcz. by high‐speed counter‐current chromatography using stepwise elution

Niu

Xie

Cai

et al. 2011

J of Separation Science

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High-speed counter-current chromatography (HSCCC) was successfully applied for the preparative separation and purification of alkaloids from Corydalis bungeana Turcz. (Kudiding in Chinese) for the first time. After the measurement of partition coefficient of seven target alkaloids in the nine two-phase solvent systems composed of CHCl3–MeOH–(0.1 M; 0.2 M; 0.3 M) HCl (4:1.5:2; 4:2:2; 4:3:2, v/v), CHCl3–MeOH–0.2 M HCl (4:2:2, v/v) and CHCl3–MeOH–0.3 M HCl (4:3:2, v/v) were finally selected for the HSCCC separation using the first upper phase as the stationary phase and the stepwise elution of the two lower mobile phases. Consequently, sanguinarine (10 mg), corynoline (25 mg), protopine (20 mg), corynoloxine (18 mg), and 12-hydroxycorynoline (8 mg) were obtained from 200 mg of crude alkaloid extracts with purities of 94–99% as determined by HPLC. Their chemical structures were characterized on the basis of 1H-NMR, 13C-NMR, and LC-ESI-Q-TOF-MS/MS analyses.

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“…The recoveries of sanguinarine and chelerythrine were 40-60% in both water and urine because these two compounds are quaternary ammonium alkaloids and were tightly absorbed onto the SPE absorbent. However, in the blood samples, the recoveries were 5-11% for sanguinarine and chelerythrine, beside the reason above-mentioned, which can be interpreted that the presence of quaternary nitrogen atom led to the high binding capacity of sanguinarine and chelerythrine to cellular proteins and DNA in the blood [28][29][30][31]. As a result, sanguinarine and chelerythrine could not be detected in the blood samples with the proposed method.…”

Section: Sample Pretreatment Proceduresmentioning

confidence: 83%

Simultaneous Determination of Toxic Alkaloids in Blood and Urine by HPLC–ESI–MS/MS

Zhang

Huang

et al. 2012

Chromatographia

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A sensitive and selective method for simultaneous determination of 29 toxic alkaloids in human blood and 31 in urine using high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry was developed and validated. The samples were diluted with 0.1 mol L -1 HCl, and the target alkaloids were purified by solid phase extraction. The separation of 31 alkaloids was carried out on a C18 column using a gradient mobile phase with 10 mmol L -1 ammonium formate in water with 0.1% formic acid and methanol at the rate of 0.25 mL min -1 . The triple-quadrupole mass spectrometer equipped with an electrospray source in the positive mode was set up in the dynamic multiple reactions monitoring mode (dynamic MRM) to detect the ion transitions of 31 alkaloids. The calibration curves were linear over a range of 0.5-400, 1-400, or 4-400 lg L -1 for target alkaloids in human blood and urine, and the correlation coefficients (r 2 ) was higher than 0.9943. The limit of determination and limit of quantification were 0.2-1 and 0.5-4 lg L -1 for blood and urine, respectively. The only exceptions were sanguinarine and chelerythrine in human blood. All the target alkaloids were stable under the test condition. In addition, the solvent effect and reconstituted solution were investigated. The method was validated and proved to be accurate and precise over the studied concentrations and suitable for poisoning diagnosis and forensic toxicology.

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Quantitative liquid chromatographic determination of sanguinarine in cell culture medium and in rat urine and plasma

Cited by 15 publications

References 17 publications

A liquid chromatographic–mass spectrometric evidence of dihydrosanguinarine as a first metabolite of sanguinarine transformation in rat

A liquid chromatographic–mass spectrometric evidence of dihydrosanguinarine as a first metabolite of sanguinarine transformation in rat

Preparative isolation of alkaloids from Corydalis bungeana Turcz. by high‐speed counter‐current chromatography using stepwise elution

Simultaneous Determination of Toxic Alkaloids in Blood and Urine by HPLC–ESI–MS/MS

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