Quantitative Light-Induced Fluorescence to Measure Enamel Remineralization in vitro

Abstract

The aim of this study was to compare the ability of quantitative light-induced fluorescence (QLF) and surface microhardness (SMH) to measure the remineralization of enamel subsurface lesions, using a pH-cycling model including treatment with 0-ppm, 550-ppm or 1,100-ppm sodium fluoride (NaF) dentifrices. Methods: Subsurface lesions were created in human enamel specimens (n = 36) and exposed to a remineralization pH-cycling model for 14 days. The pH-cycling model was performed in an automated system where specimens were subjected to a demineralizing solution for 20 min and treatment for 1 min and were then remineralized for 7 h 39 min, 3 times daily. The treatments consisted of 3 NaF, silica-containing dentifrices (0 ppm F; 550 ppm F; 1,100 ppm F). The outcome variables were: change from baseline in surface hardness and percentage change from baseline in fluorescence. An ANCOVA explored differences between different treatment groups (at the p < 0.05 level). Associations between QLF and SMH were evaluated using Spearman's correlation coefficient. Results: The percentage SMH changes were 14.9 ± 2.1%, 56.6 ± 9.6% and 103.9 ± 14.6% for the 0-, 550- and 1,100-ppm F dentifrices, respectively. The percentage fluorescence changes were 15.6 ± 7.1%, 59.8 ± 11.9% and 85 ± 13.2%, respectively. The differences between all pairwise comparisons were statistically significant for both methods (p = 0.001). QLF correlated with SMH (r = 0.67). Conclusions: Both the SMH and QLF methods demonstrated a significant F dose response for toothpaste in this in vitro remineralization model, and both methods were able to distinguish treatments with different F levels.

Effects of rinsing with arginine bicarbonate and urea solutions on initial enamel lesions in situ

“…It has been employed in caries research and has a broad range of applications, including the assessment of in vitro and in vivo demineralization and remineralization (De Josselin De Jong et al , ; van der Veen and De Josselin De Jong, ; Feng et al , ). Comparative studies have shown a good correlation between the results of QLF and those of other techniques, including transverse microradiography, which is considered the gold standard (Hafstrom‐Bjorkman et al , ; Ando et al , ; Gomez et al , ).…”

Desempenho de métodos baseados em indução de fluorescência na avaliação da remoção de dentina cariada

“…O QLF vem sendo amplamente estudado por se tratar de uma ferramenta quantitativa para detecção das lesões de cárie [10][11][12][13]20 . 27 obtiveram em seu estudo uma correlação interexaminador de 0,48 para o DDpen, assim como Yazici et al 28 encontraram valores entre 0,46 e 0,89.…”

“…A sua capacidade de diagnóstico baseia-se no mecanismo em que a intensidade de fluorescência natural de um dente é diminuída por dispersão devido a uma lesão cariosa; assim, a fluorescência emitida apresenta uma relação direta com o conteúdo mineral do esmalte 10 . Estudos têm mostrado sua utilização para detecção de lesões cariosas incipientes em dentes decíduos 11 15 .…”

Validation of quantitative light‐induced fluorescence‐digital in the quantification of demarcated hypomineralized lesions of enamel

“…However, it has been hypothesized that dentine and the DEJ are the source of fluorescence . Fluorescence change owing to enamel and dentine alterations could be used as a surrogate measure for MD variation, and it could be a useful tool to quantify MD changes in clinical settings . The QLF technology has been used for the quantification of fluorescence changes in early stages of carious lesions and in fluorosis .…”