Quantitative investigations on moving chelation boundary within a continuous EDTA‐based sample sweeping system in capillary electrophoresis

Fan, Liu-Yin; Li, Chenjia; Zhang, Wei; Cao, Cheng-Xi; Zhou, Pei; Deng, Zi-xin

doi:10.1002/elps.200800051

Cited by 20 publications

(31 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The second example is that the method of MRB can be well used for sample stacking [19,22]. And the third is that the MCB concept, one kind of MRB, can be used for the quantitative illumination of an EDTA-based sweeping in CE [19,25]. Furthermore, we [19,21] unveiled that the predictions with the equation of MRB were quantitatively in coincidence with the computation with the KRF and the experiments of electrically controlled electro-focusing [20].…”

Section: Introductionmentioning

confidence: 55%

See 1 more Smart Citation

Theoretical and experimental investigations on relationship between Kohlrausch regulating function/inequality and moving reaction boundary in electrophoresis

Zhang¹,

Jin²,

Fan³

et al. 2009

J of Separation Science

Self Cite

View full text Add to dashboard Cite

Kohlrausch regulating function (KRF) has been well defined from a moving boundary system for over 50 years. Recently, a series of theoretical and experimental studies were carried out on the concept of moving reaction boundary (MRB), including moving precipitate boundary, moving neutralization boundary and moving chelation boundaries (MCB). However, there has not been any direct evidence to show whether the KRF has validity for a MRB or not. In this paper, the KRF is derived from the equation of MRB under condition of nonzero boundary velocity. The result directly shows the relation between the KRF and a MRB. The relation is verified by some experimental and simulation results on MCB and moving neutralization boundary as well as Svensson's classic IEF. Furthermore, a Kohlrausch inequality was defined from the equation of MRB and was further proved by numerous data. The results show that we can use the KRF for the investigation on a MRB under the condition of nonzero boundary velocity, whereas we ought to notice with great attention that the KRF does possibly have invalidity under some special cases, such as stationary electrolysis and IEF. The findings hold an evident significance to the study of a MRB with the KRF.

show abstract

Section: Introductionmentioning

confidence: 55%

“…2 can be calculated from a standard calibration curve in ref. [25] based on peak height rather than peak area. It is computed from the calibration that the concentrations of Cu(II)-EDTA for 2.0, 1.0, 0.6,0.10, 0.01 and 0.001 mM Cu(II) are 28.6, 31.6, 30.2, 14.8, 1.90 and 1.84 mM, respectively.…”

Section: Agreement Between Krf and Mcb Experimentsmentioning

confidence: 99%

Theoretical and experimental investigations on relationship between Kohlrausch regulating function/inequality and moving reaction boundary in electrophoresis

Zhang¹,

Jin²,

Fan³

et al. 2009

J of Separation Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…Based on MRB principles Palmer et al invented the technique of sample condensation with sample sweeping in CE [22,23]. Cao's group further developed the moving precipitate boundary [24], MNB [25,26] and moving chelation boundary [27]. Breadmore et al simulated the dynamic stacking with a transient MNB [28,29].…”

Section: Introductionmentioning

confidence: 99%

Recursion approach for moving neutralization boundary formed on IPG strips Part I: With strong alkali rehydration buffer

Liang¹,

Chen²,

Tian³

et al. 2009

Electrophoresis

View full text Add to dashboard Cite

Moving neutralization boundary (MNB) is an important foundation to understand and improve IEF. However, there are obstacles in theoretical predictions of MNB on IPG strips due to the unknown local concentrations of carrier ampholytes on commercial IPG strips and the time-varying boundary velocities. We introduce a recursion approach to extend the current MNB theories into the space-time varying MNB system. The recursion approach emphasizes the localizability of physicochemical parameters in the discrete time intervals and local positions in Lagrangian coordinates, such as local concentrations of carrier ampholytes, local OH concentrations, local boundary velocities, local judgments, etc. The boundary-position recursion equation in a complete time sequence was presented to quantitatively predict the MNB position-time curves by distinguishing three kinds of titration cases according to NaOH concentrations in rehydration buffers. The theoretical position-time curves and local relative judgments of boundaries were satisfactorily validated by corresponding images of boundary migrations achieved from the IPG-MNB experiments with the some typical NaOH concentrations-bromophenol blue-rehydration buffers on pH 4-7 IPG strips. The results achieved herein have evident significances to the development of moving reaction boundary and IEF.

show abstract

“…Later, Fan et al. revealed the mechanism of sample stacking by using the concept of chelation or supramolecular boundary .…”

Section: Introductionmentioning

confidence: 99%

Online stacking of carboxylated magnetite core–shell nanoparticles in capillary electrophoresis

Baron

Dolanská

Medříková

et al. 2017

J of Separation Science

View full text Add to dashboard Cite

The stacking effect on carboxylated magnetite core-shell nanoparticles using sodium borate buffer pH 9.5 as the background electrolyte is presented. The ionic strength of the background electrolyte ranged from 5 to 100 mM, and the ionic strength of a sample zone ranged from 5 to 100 mM. Moreover, water was used as the sample dispersant. Both stacking and de-stacking effects were observed when conductivities of the sample zone and the background electrolyte differed. An explanation of carboxylated magnetic core-shell nanoparticles behavior was suggested based on the Derjaguin-Landau-Verwey-Overbeek theory supposing that the aggregation point is defined by the energetic barrier as the sum of energies given by electrostatic interactions and Van der Waals interactions. Moreover, the stacking conditions were applied for the evaluation of the lowest detectable dilution of magnetic nanoparticles. The carboxylated magnetic nanoparticles were dispersed in 10 mM borate/NaOH pH 9.5 and injected for 60 s to the background electrolyte composed of 100 mM borate/NaOH pH 9.5 that allowed the detection of 100-fold diluted nanoparticles.

show abstract

Quantitative investigations on moving chelation boundary within a continuous EDTA‐based sample sweeping system in capillary electrophoresis

Cited by 20 publications

References 34 publications

Theoretical and experimental investigations on relationship between Kohlrausch regulating function/inequality and moving reaction boundary in electrophoresis

Theoretical and experimental investigations on relationship between Kohlrausch regulating function/inequality and moving reaction boundary in electrophoresis

Recursion approach for moving neutralization boundary formed on IPG strips Part I: With strong alkali rehydration buffer

Online stacking of carboxylated magnetite core–shell nanoparticles in capillary electrophoresis

Contact Info

Product

Resources

About