Quantitative importance of non-skeletal-muscle <i>N</i>τ-methylhistidine and creatine in human urine

Afting, E G; Bernhardt, Wolfgang; Janzen, R. W. C.; Röthig, H J

doi:10.1042/bj2000449

Cited by 45 publications

(12 citation statements)

References 18 publications

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“…The values for BUN, K, uric acid, Ca, Ca ++ , and Pi were significantly higher in pigs less than six months of age, a possible indication of poor kidney function (Figure 1). However, the level of Cr, an indicator of kidney function, was significantly lower in young pigs, possibly due to the low muscle volume [21]. Additionally, TIBC, UIBC and RDW were significantly higher in young pigs, but RBC, Hb, HCT, MCHC and MCV were significantly lower in young pigs (Figure 2).…”

Section: Discussionmentioning

confidence: 99%

Analysis of reference interval and age-related changes in serum biochemistry and hematology in the specific pathogen free miniature pig

et al. 2012

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We analyzed 72 items related to serum biochemistry and hematology in 85 specific pathogen-free (SPF) Seoul National University (SNU) miniature pigs aged 1- to 36-months which originated from a Minnesota miniature pig. Almost all examined items were similar between male and female pigs. However, some items such as Cr level, B/C ratio, C.R.F, LDH, LAP and T4 were significantly different between male and female pigs (P<0.05). Thirty four examined items showed age-related changes, and the significant changes were observed in animals less than six months old. The values for BUN, K, uric acid, Ca, Ca++, and Pi were significantly higher in pigs younger than six months of age, which might reflect poor kidney function in young pigs. Additionally, TIBC, UIBC and RDW were significantly higher in young pigs, and RBC, Hb, HCT, MCHC and MCV were significantly lower in young pigs, thus indicating a similar physiology of iron deficiency anemia. These age-related specific phenotypes seemed to be normal, but it should be considered in the long-term experiment using the young pigs. In conclusion, in this study, we defined the normal reference intervals for SPF SNU miniature pigs, and we also determined that there are some physiological differences between the pig genders and ages. This study provides fundamental data for use in experiments involving SPF SNU miniature pigs.

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Section: Discussionmentioning

confidence: 99%

Analysis of reference interval and age-related changes in serum biochemistry and hematology in the specific pathogen free miniature pig

et al. 2012

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“…In addition to the amount of myofibrillar proteins, the turnover rate determines the amount of 3MH that is produced. Since intestine is thought, in particular, to have a high turnover rate, it might contribute significantly to Wb 3MH production [37][38][39][40][41][42].…”

Section: Wb Versus Organ Levelmentioning

confidence: 99%

Measuring whole-body actin/myosin protein breakdown in mice using a primed constant stable isotope-infusion protocol

et al. 2003

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To measure actin/myosin protein breakdown, the 24 h excretion of N (tau)-methylhistidine (3MH) is used. However, in mice, this method is invalid. Therefore we have developed a liquid chromatography-MS technique to measure the tracer/tracee ratio and concentration of 3MH in plasma, enabling an in vivo primed constant infusion protocol with a deuterated stable isotope of 3MH. We tested this model by giving a primed constant infusion of L-[3-methyl-(2)H(3)]histidine, L-[phenyl-(2)H(5)]phenylalanine and L-[phenyl-(2)H(2)]tyrosine to three anaesthetized experimental groups: mice receiving saline intraperitoneally (i.p.) (CON), mice receiving saline i.p. and starved for 9 h (STA), and mice receiving lipopolysaccharide i.p. and starved for 9 h (STA+LPS). The contribution of myofibrillar to total protein breakdown was significantly lower in the STA group than the CON group (30+/-4% and 54+/-14% respectively; P <0.05), and was significantly higher in the STA+LPS group than the STA group (52+/-7% and 30+/-4% respectively; P <0.05). Whole-body myofibrillar protein breakdown, total protein breakdown, protein synthesis and net protein breakdown were not different between the groups. We conclude that this in vivo primed constant stable isotope-infusion protocol can give valuable information about the role of actin/myosin protein breakdown in mice.

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“…There is growing evidence that this is not so. For example, Afting and co-workers showed that there was considerable residual excretion of 3-methylhistidine and creatinine in a paralysed patient, who on later autopsy was declared to have no detectable skeletal muscle [37]. The 3-methylhistidine excretion could have accounted for between 20 and 80% of the range of excretion in normal subjects.…”

Section: Evidence From Studies In Manmentioning

confidence: 99%

3-Methylhistidine Excretion and the Urinary 3-Methylhistidine/Creatinine Ratio Are Poor Indicators of Skeletal Muscle Protein Breakdown

1983

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Quantitative importance of non-skeletal-muscle Nτ-methylhistidine and creatine in human urine

Cited by 45 publications

References 18 publications

Analysis of reference interval and age-related changes in serum biochemistry and hematology in the specific pathogen free miniature pig

Analysis of reference interval and age-related changes in serum biochemistry and hematology in the specific pathogen free miniature pig

Measuring whole-body actin/myosin protein breakdown in mice using a primed constant stable isotope-infusion protocol

3-Methylhistidine Excretion and the Urinary 3-Methylhistidine/Creatinine Ratio Are Poor Indicators of Skeletal Muscle Protein Breakdown

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