Quantitative immunoassay for soya protein in raw and sterilized meat products

Abstract

An enzyme-linked immunosorbent assay (ELISA) is described for the quantitative analysis of soya protein in meat products. The assay is applicable to raw and sterilized products and is not dependent on soya variety and type of soya ingredient (concentrate, isolate, etc.). Therefore, the assay can be applied without knowledge of product composition, heat pretreatment and other processing conditions. The ELISA is specific for soya; no interference of other product components has been found. The detection limit of the ELISA is 0.5% soya protein. Qualitative analysis by immunoblotting is possible at much lower concentrations. The antibodies used are specific for sodium dodecyl sulphate (SDS) denatured soya protein subunits. The products are extracted with SDS and 2-mercaptoethanol, which guarantees optimum recovery of the protein components. Product extracts can be analysed directly by ELISA without removal of the SDS by using nitrocellulose as a solid phase.

Immunohistochemical detection of soya protein - optimisation and verification of the method

“…In the second group, soya was detected in all model samples containing different types of soya additive (texturate, concentrate, soya flour, isolate). It was confirmed that immunochemical methods can be used for the detection of different kinds and types of soya protein (Ravestein & Driedonks 1986;Yasumoto et al 1990). …”

Detection of Soybean Proteins in Fermented Soybean Products by Using Heating Extraction

“…Previous studies have detected soybean proteins by using ELISA based on antibodies to soybean proteins such as the trypsin inhibitor 7S‐conglycinin and 11S glycinin (Hitchcock and others ; Ravestein and Driedonks ; Brandon and others ; Koppelman and others ). However, to our knowledge there have been no reports of the successful detection of soybean proteins in fermented soybean products.…”

Soy protein: a food allergen frequently used in the preparation of meat products

“…Another alternative was indirect determination of soy protein content using the quantification of the phytic acid content, which is bound to plant proteins 50 . Studies describing the use of immunological analysis for the detection and quantification of plant proteins in meat products have also been reported 51,52,53,54,55 .…”