Quantitative imaging of cochlear soft tissues in wild-type and hearing-impaired transgenic mice by spectral domain optical coherence tomography

Gao, Simon S.; Xia, Anping; Tao, Yuan; Raphael, Patrick D.; Shelton, Ryan L.; Applegate, Brian E.; Oghalai, John S.

doi:10.1364/oe.19.015415

Cited by 49 publications

(35 citation statements)

References 35 publications

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“…Previously, we described our spectral domain OCT system for in vitro cochlear imaging [13]. We have now modified it to improve our ability to collect in vivo vibrometric data.…”

Section: Oct System Descriptionmentioning

confidence: 99%

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In vivo vibrometry inside the apex of the mouse cochlea using spectral domain optical coherence tomography

Gao

Raphael

Wang

et al. 2013

Biomed. Opt. Express

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Sound transduction within the auditory portion of the inner ear, the cochlea, is a complex nonlinear process. The study of cochlear mechanics in large rodents has provided important insights into cochlear function. However, technological and experimental limitations have restricted studies in mice due to their smaller cochlea. These challenges are important to overcome because of the wide variety of transgenic mouse strains with hearing loss mutations that are available for study. To accomplish this goal, we used spectral domain optical coherence tomography to visualize and measure sound-induced vibrations of intracochlear tissues. We present, to our knowledge, the first vibration measurements from the apex of an unopened mouse cochlea. propagation and reduced endocochlear potential associated with cochlear dysplasia in the BETA2/NeuroD1 null mouse," J. Assoc. Res. Otolaryngol. 8(4), 447-463 (2007). 22. C. C. Liu, S. S. Gao, T. Yuan, C. Steele, S. Puria, and J. S. Oghalai, "Biophysical mechanisms underlying outer hair cell loss associated with a shortened tectorial membrane," J.

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“…Previously, we described our spectral domain OCT system for in vitro cochlear imaging [13]. We have now modified it to improve our ability to collect in vivo vibrometric data.…”

Section: Oct System Descriptionmentioning

confidence: 99%

“…Previous OCT imaging experiments by our lab and others have shown it to be effective in resolving soft tissues within the cochlea, including the various substructures that compose the organ of Corti [13][14][15][16]. Vibration measurements have been made using OCT within the opened guinea pig cochlea in vivo [17,18].…”

Section: Introductionmentioning

confidence: 99%

In vivo vibrometry inside the apex of the mouse cochlea using spectral domain optical coherence tomography

Gao

Raphael

Wang

et al. 2013

Biomed. Opt. Express

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“…However, some recordings have been made from the top surface of the organ of Corti in the guinea pig cochlear apex using LDV (12)(13)(14). More recently, optical coherence tomography (OCT) has been used to study how regions within the organ of Corti other than the BM vibrate because it permits depth-resolved measurements inside tissue (15)(16)(17)(18). These data have indicated that this complex structure does not vibrate as a rigid body (19,20).…”

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confidence: 99%

Noninvasive in vivo imaging reveals differences between tectorial membrane and basilar membrane traveling waves in the mouse cochlea

Lee

Raphael

Park

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Sound is encoded within the auditory portion of the inner ear, the cochlea, after propagating down its length as a traveling wave. For over half a century, vibratory measurements to study cochlear traveling waves have been made using invasive approaches such as laser Doppler vibrometry. Although these studies have provided critical information regarding the nonlinear processes within the living cochlea that increase the amplitude of vibration and sharpen frequency tuning, the data have typically been limited to point measurements of basilar membrane vibration. In addition, opening the cochlea may alter its function and affect the findings. Here we describe volumetric optical coherence tomography vibrometry, a technique that overcomes these limitations by providing depthresolved displacement measurements at 200 kHz inside a 3D volume of tissue with picometer sensitivity. We studied the mouse cochlea by imaging noninvasively through the surrounding bone to measure sound-induced vibrations of the sensory structures in vivo, and report, to our knowledge, the first measures of tectorial membrane vibration within the unopened cochlea. We found that the tectorial membrane sustains traveling wave propagation. Compared with basilar membrane traveling waves, tectorial membrane traveling waves have larger dynamic ranges, sharper frequency tuning, and apically shifted positions of peak vibration. These findings explain discrepancies between previously published basilar membrane vibration and auditory nerve single unit data. Because the tectorial membrane directly overlies the inner hair cell stereociliary bundles, these data provide the most accurate characterization of the stimulus shaping the afferent auditory response available to date.hearing | cochlea | mechanics | vibrometry | auditory system

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“…The novel improvements in imaging technologies resulted in the introduction of experimental imaging modalities to evaluate the intracochlear microanatomy such as OCT and SLOT (7,(11)(12)(13)(14). These high resolution modalities are nondestructive and facilitate near real time evaluation of the structures.…”

Section: Discussionmentioning

confidence: 99%

Preparation of Human Inner Ear Structures for High Resolution Imaging Studies

et al. 2017

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Background: Introduction of the novel imaging modalities such as optical coherence tomography and scanning laser optical tomography allow the morphological and functional evaluation of the intracochlear structures up to the histological details. The prerequisite of these modalities is the preparation of the membranous labyrinth by removing the dense otic capsule to enhance the optical penetration depth. In the present study, a combination of the chemical decalcification and bone drilling method was explained for the preparation of the human inner ear structures for further studies. Methods: In this study, nine human temporal bones were used and trimmed in cubes containing middle and inner ear structures. The samples were immersed in 5% nitric acid, 20% EDTA, and 10% EDTA solutions, respectively. The samples were brought out and rinsed every 90 minutes, and mechanical removal of the softened bone was performed with surgical drill system until the complete decalcification of the samples was confirmed by X-ray imaging. The prepared samples were evaluated by microcomputed tomography imaging for anatomical distortions caused by the preparation process.

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Quantitative imaging of cochlear soft tissues in wild-type and hearing-impaired transgenic mice by spectral domain optical coherence tomography

Cited by 49 publications

References 35 publications

In vivo vibrometry inside the apex of the mouse cochlea using spectral domain optical coherence tomography

In vivo vibrometry inside the apex of the mouse cochlea using spectral domain optical coherence tomography

Noninvasive in vivo imaging reveals differences between tectorial membrane and basilar membrane traveling waves in the mouse cochlea

Preparation of Human Inner Ear Structures for High Resolution Imaging Studies

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