Quantitative HPLC-ICP-MS analysis of antimonyredox speciation in complex sample matrices: new insights into the Sb-chemistry causing poor chromatographic recoveries

Hansen, Claus; Schmidt, Bjørn; Larsen, Erik Huusfeldt; Gammelgaard, Bente; Stürup, Stefan; Hansen, Hanna E.

doi:10.1039/c0an00796j

Cited by 14 publications

(13 citation statements)

References 29 publications

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“…Instead, another chromatographic approach was applied with acid hydrolysis of the samples followed by chromatography in a mobile phase of 100 mM citrate pH 4.5 (Fig. 1); a method developed specifically for determination of Sb oxidation state in complex sample matrices [21]. With this methodology, the extracts on average had a recovery of 86% and showed no unidentified peaks.…”

Section: Resultsmentioning

confidence: 99%

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Reduction of Sb(V) in a Human Macrophage Cell Line Measured by HPLC-ICP-MS

Hansen

et al. 2011

Biol Trace Elem Res

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Drugs based on pentavalent antimony are first-line treatment of the parasite disease leishmaniasis. It is generally believed that Sb(V) acts as a prodrug, which is activated by reduction to Sb(III); however, the site of reduction is not known. It has been hypothesised that the reduction takes place in the parasites' host cells, the macrophages. In this study, the human macrophage cell line Mono Mac 6 was exposed to Sb(V) in form of the drug sodium stibogluconate (Pentostam™). Cell extracts were analysed for Sb species by high-performance liquid chromatography with inductively coupled plasma-mass spectrometry detection. We found that Sb(V) is actually reduced to Sb(III) in the macrophages; up to 23% of the intracellular Sb was found as Sb(III). Transfer of the cells to Sb-free medium rapidly decreased their Sb(V) and Sb(III) content. Induction of the cell's production of reactive oxygen species did not have any marked effect on the intracellular amounts of Sb(III).

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Section: Resultsmentioning

confidence: 99%

“…(This sample pre-treatment procedure cause hydrolysis of any polymeric antimony species and allows for a complete chromatographic Sb recovery, cf. [21]. )…”

Section: Reduction Experimentsmentioning

confidence: 91%

Reduction of Sb(V) in a Human Macrophage Cell Line Measured by HPLC-ICP-MS

Hansen

et al. 2011

Biol Trace Elem Res

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“…This would result in bias due, respectively, to co-precipitation and/or sorption of the analyte on the newly formed phases or to modification of the original complexation equilibrium involving the studied TE. For example, the addition of EDTA in the mobile phase may induce a dissociation of weak complexes of antimony (Sb) and organic acids (Hansen et al, 2011). Mobile-phase composition and pH should thus be compatible with the AD sample.…”

Section: Te Speciation In Liquid Samplesmentioning

confidence: 99%

Biogeochemistry of trace elements in anaerobic digesters

2019

Trace Elements in Anaerobic Biotechnologies

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Essential trace elements (TE) are a prerequisite that ensures optimal performance of the anaerobic digestion (AD) process. However, finding the proper way to deliver these micronutrients to microbial communities is not an easy task. The chemical speciation of TE and the complex environment characterizing AD play a critical role in their mobility, bioavailability, and toxicity. These aspects are particularly

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“…For instance, Sb/Cr complexes with organic acids are weaker in contrast to the corresponding EDTA complex. EDTA can be used on the one hand to preserve the original oxidation state of the element (Hansen et al 2011;Markiewicz et al 2015). On the other hand, however, the true original speciation information may be lost, since more labile complexes (e.g.…”

Section: Determination Of Dissolved Te Speciationmentioning

confidence: 99%

“…On the other hand, however, the true original speciation information may be lost, since more labile complexes (e.g. Sb(III)-citrate, tartrate (Hansen et al 2011)) dissociate in favour of the EDTA complex.…”

Section: Determination Of Dissolved Te Speciationmentioning

confidence: 99%

Methodological approaches for fractionation and speciation to estimate trace element bioavailability in engineered anaerobic digestion ecosystems: An overview

Hullebusch¹,

Guibaud

Simon

et al. 2016

Critical Reviews in Environmental Science and Technology

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Optimal supply of trace elements (TE) is a prerequisite for microbial growth and activity in anaerobic digestion (AD) bioprocesses. However, the required concentrations and ratios of essential TE for AD biotechnologies strongly depend on prevailing operating conditions as well as feedstock composition. Furthermore, TE in AD bioreactors undergo complex physicochemical reactions and may be present as free ions, complex bound or as precipitates depending on pH, or on the presence of sulfur compounds or organic macromolecules. To overcome TE deficiency, various commercial mineral products are typically applied to AD processes. The addition of heavy metals poses the risk of overdosing operating systems, which may be toxic to microbial consortia and ultimately the environment. Adequate supplementation, therefore, requires appropriate knowledge not only about the composition, but also on the speciation and bioavailability of TE. However, very little is yet fully understood on this specific issue. Evaluations of TE typically only include the measurement of total TE concentrations but do not consider the chemical forms in which TE exist. Thus detailed information on bioavailability and potential toxicity cannot be provided. This review provides an overview of the state of the art in approaches to determine bioavailable TE in anaerobic bioprocesses, including sequential fractionation and speciation techniques. Critical aspects and considerations, including with respect to sampling and analytical procedures, as well as mathematical modeling, are examined. The approaches discussed in this review are based on our experiences and on previously published studies in the context of the “COST Action 1302: European Network on Ecological Roles of Trace Metals in Anaerobic Biotechnologies.

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Quantitative HPLC-ICP-MS analysis of antimonyredox speciation in complex sample matrices: new insights into the Sb-chemistry causing poor chromatographic recoveries

Cited by 14 publications

References 29 publications

Reduction of Sb(V) in a Human Macrophage Cell Line Measured by HPLC-ICP-MS

Reduction of Sb(V) in a Human Macrophage Cell Line Measured by HPLC-ICP-MS

Biogeochemistry of trace elements in anaerobic digesters

Methodological approaches for fractionation and speciation to estimate trace element bioavailability in engineered anaerobic digestion ecosystems: An overview

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