Quantitative HPLC Determination of the Antioxidant Activity of Capsaicin on the Formation of Lipid Hydroperoxides of Linoleic Acid:  A Comparative Study against BHT and Melatonin

Abstract

The antioxidant activity of capsaicin, as compared to BHT and melatonin, was determined by the direct measurement of lipid hydroperoxides formed upon linoleic acid autoxidation initiated by AIBN. The formation of four isomeric lipid hydroperoxides was detected after reverse-phase HPLC separation. Data from three detectors, UV absorption, glassy carbon electrode electrochemical detection, and postcolumn chemiluminescence using luminol, were compared. Capsaicin was more effective than melatonin in suppressing the formation of lipid hydroperoxides but not as effective as BHT. The formation of capsaicin and BHT dimers was observed during oxidation, and the dimers were characterized using APCI MS(n).

Protective Effects of Capsaicin against Cisplatin-Induced Nephrotoxicity in Rats

“…4,24) Cap and its analogues have more effective antioxidant activity. [7][8][9][10]25) Cap was also found to scavenge radicals at/near the membrane surface and in the interior of the phospholipid membrane.…”

“…It has been used as a tool in the study of pain sensations which are caused by stimulation of Cap receptor or vanilloid receptor 1, an ion channel protein expressed by nociceptive primary afferent neurons. 5,6) On the other hand, Cap potentially inhibits the peroxidation of various lipids [7][8][9][10] and generates reactive oxygen species in rat peritoneal macrophages.…”

“…It has been used as a tool in the study of pain sensations which are caused by stimulation of Cap receptor or vanilloid receptor 1, an ion channel protein expressed by nociceptive primary afferent neurons. 5,6) On the other hand, Cap potentially inhibits the peroxidation of various lipids [7][8][9][10] and generates reactive oxygen species in rat peritoneal macrophages.11) However, the mechanism responsible for the potent inhibitory effects of Cap on lipid peroxidation in vivo is still unclear.Several studies have examined the effects of decreased lipid peroxidation and nephrotoxicity induced by CDDP using various agents including antioxidants. [12][13][14] The purpose of the present study was to investigate whether the administration of Cap exerts any protective effect against cisplatin-induced lipid peroxidation and nephrotoxicity in rats.…”

Ultrasound-assisted extraction of capsaicinoids from peppers

“…Among these activities, capsaicinoids act as powerful antioxidants [5], present anti-mutagenic and anti-tumoral properties [6,7], function as topical analgesics against pain [8], have anti-inflammatory properties [9] and stimulate the cardiovascular and respiratory systems [10].…”

Determination of capsaicinoids in peppers by microwave-assisted extraction–high-performance liquid chromatography with fluorescence detection

“…In particular, they are antimutagenic and antitumoral compounds [4,5], antioxidants [6,7], and are commonly utilised as topical analgesics for many painful clinical conditions [8]. * Corresponding author.…”