Quantitative HPLC Determination and Extraction of Anthraquinones in Senna alata Leaves

Abstract

A reversed-phase high-performance liquid chromatographic method is described for the simultaneous determination of four anthraquinones: rhein, aloe-emodin, emodin, and chrysophanol in Senna alata leaves. The method involves the use of a TSK-gel ODS-80Tm column (5 microm, 4.6 x 150 mm) at 25 degrees C with the mixture of methanol and 2% aqueous acetic acid (70:30, v/v) as the mobile phase and detection at 254 nm. The parameters of linearity, precision, accuracy, and specificity of the method were evaluated. The recovery of the method is 100.3-100.5%, and linearity (r(2) > 0.9998) was obtained for all anthraquinones. A high degree of specificity as well as repeatability and reproducibility (relative standard deviation values less than 5%) were also achieved. The solvent for extraction of anthraquinones from S. alata leaves was examined in order to increase the anthraquinone content of the leaf extract. It was found that a solution of 5% hydrochloric acid (v/v), 5% ferric chloride (w/v), and 15% water in methanol (v/v) was capable of increasing the anthraquinone content in the leaf extract up to 1.67% (w/w).

Antibacterial-guided isolation of constituents from <i>Senna alata </i>leaves with a particular reference against Multi-Drug-Resistant <i>Vibrio cholerae </i>and <i>Shigella flexneri</i>

“…Fractionation and purification of A and C yielded to the isolation of three bioactive compounds namely Aloe-emodin (1) (Panichayupakaranant et al, 2009), Kaempferol (2) (Melo et al, 2009) and Luteolin (3) (Lie-Chwen et al, 2015) ( Figure 1). The antibacterial activity of the isolated compounds was examined by broth microdilution assay against six bacterial strains.…”

Estimation of Rhein from Cassia fistula Linn. using validated HPTLC method

“…The method was also found sensitive with a limit of detection and limit of quantification values 0.5 μg/mL and 5.0 μg/mL respectively, and the results were incompliance with other published reports. [12][13][14] The average recovery for quality control samples of Rhein was found to be 101.23% [ Table 1]. The method was also found rugged for the parameters such as change in analysts, change in mobile phase composition and change in spotting volume.…”

“…Estimation of Rhein has been carried out using the various analytical techniques. A reversed-phase high-performance liquid chromatographic method is described for the determination of Rhein in Senna alata leaves [12] and Cassia alata leaves. [13] Recently, a validated TLC technique has been reported for quantitation of Rhein from the aqueous extract of C. fistula fruit pulp.…”

Reduction ofCandida tropicalisbiofilm by photoactivation of aHeterophyllaea pustulataextract

“…The HPLC column and the solvent system used in the experiment were routinely applied to the separation of AQs in a complicated plant matrix (Dongxiu et al 2009;Panichayupakaranant et al 2009;Wei et al 2013). In our experiment, the gradient solvent system and flow rate were optimized in order to obtain a good separation and resolution of target compounds in the chromatograms.…”