Quantitative histological studies on age changes in bone

Dunnill, M. S.; Anderson, John A.; Whitehead, R.

doi:10.1002/path.1700940205

Cited by 250 publications

(116 citation statements)

References 16 publications

(4 reference statements)

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“…In addition, we took partial correlations with the age or E max as the controlling variable. The positive correlation between LWR and E max retained its significance after controlling the age effect, which suggested the correlation of LWR and E max was not 1 H MRS data and perfusion parameters among the 50 female subjects. The E max has a negative correlation with LWR and age, while LWR has a positive correlation with age, and lipid LW has a negative correlation with age.…”

Section: Discussionmentioning

confidence: 90%

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Correlation between the bone marrow blood perfusion and lipid water content on the lumbar spine in female subjects

Chen

Shih

2006

Magnetic Resonance Imaging

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Purpose: To assess the marrow lipid water ratio (LWR) and spectral line width (LW) of lumbar vertebra by proton MR spectroscopy ( 1 H MRS) and correlate with the marrow blood perfusion (MBP) by contrast-enhanced dynamic MRI (dMRI) in female subjects. Materials and Methods:A total of 50 female subjects were included. Single-voxel 1 H MRS was measured at the L3 vertebrae. In the dMRI study, the maximum enhancement percentage (E max ) and enhancement slope were calculated from time-intensity curves. Pearson's correlation was calculated to explore the association of 1 H MRS, age, and perfusion parameters. Partial correlation was then used to control confounder effect.Results: LWR was negatively correlated with E max (r ϭ -0.72; P Ͻ 0.0001), but positively correlated with age (r ϭ 0.63; P Ͻ 0.0001 ). Lipid LW was negatively correlated with age (r ϭ -0.33; P Ͻ0.05), but positively correlated with E max (r ϭ 0.4; P Ͻ 0.05). After adjusting for the age effect by partial correlation, marrow LWR still negatively correlated with E max (r ϭ -0.63; P Ͻ 0.0001). After adjusting for the E max effect, marrow LWR still positively correlated with age (r ϭ 0.37; P Ͻ 0.05). Conclusion:After controlling the age factor, the LWR and water LW has significant negative correlation with vertebral BMP. Further investigation is needed to explore the relationship between bone marrow fat metabolism and angiogenesis.

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Section: Discussionmentioning

confidence: 90%

“…Quantitative histologic studies on agerelated changes in bone have shown that the change from hematopoietic to fatty marrow is gradual, steady, and progressive (1). In the histomorphometric study conducted by Burkhardt et al (2), the bone marrow capillaries decrease in aging people.…”

mentioning

confidence: 99%

Correlation between the bone marrow blood perfusion and lipid water content on the lumbar spine in female subjects

Chen

Shih

2006

Magnetic Resonance Imaging

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“…There is considerable evidence demonstrating a reciprocal relationship between bone volume and adipose volume in the marrow (7,8). One likely model is that PPAR␥ activation directly suppresses osteogenesis, because stable transfection of PPAR␥ and its activation with a TZD suppressed expression of runx2, type I collagen, and osteocalcin in stromal cells (9).…”

Section: Discussionmentioning

confidence: 99%

“…Clinical studies show that the decrease in bone volume associated with osteoporosis or aging is accompanied by an increase in marrow adipose tissue (7,8). Although the molecular mechanisms underlying this reciprocal relationship are not well understood, considerable work has established that activation of peroxisome proliferator-activated receptor ␥ (PPAR␥) 2 induces adipocyte differentiation (9) and inhibits osteoblastic differentiation of mesenchymal stem cells (10).…”

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confidence: 99%

Skeletal Consequences of Deletion of Steroid Receptor Coactivator-2/Transcription Intermediary Factor-2

Mödder

Monroe

Fraser

et al. 2009

Journal of Biological Chemistry

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Both estrogen receptor (ER) and peroxisome proliferator-activated receptor ␥ (PPAR␥) regulate bone metabolism, and because steroid receptor coactivator (SRC)-2 (TIF-2) enhances ER and PPAR␥ activity, we examined the consequences of deletion of SRC-2 on bone using SRC-2 knock out (KO) mice. Loss of SRC-2 resulted in increased bone mass, with SRC-2 KO mice having 80% higher trabecular bone volume as compared with wild type mice. SRC-2 KO mice also had a marked decrease (by 50%) in bone marrow adipocytes. These data suggested that marrow precursor cells in the SRC-2 KO mice may be resistant to the inhibitory effects of endogenous PPAR␥ ligands on bone formation. Consistent with this, compared with cultures from wild type mice, marrow stromal cultures from SRC-2 KO mice formed significantly more mineralized nodules (by 3-fold) in the presence of the PPAR␥ agonist, rosiglitazone. Using chromatin immunoprecipitation analysis, we demonstrated that in bone marrow stromal cells, loss of SRC-2 leads to destabilization of the transcription complex at the peroxisome proliferator response elements of a number of PPAR␥ target genes, resulting in an overall decrease in the expression of adipocyte-related genes and a marked decrease in adipocyte development. Using ovariectomy with or without estrogen replacement, we also demonstrated that SRC-2 KO mice were partially resistant to the skeletal actions of estrogen. Collectively, these findings indicate that loss of SRC-2 leads to partial skeletal resistance to the ER and PPAR␥, but resistance to PPAR␥ is dominant, leading to increased bone mass. Modulating SRC-2 action may, thus, represent a novel therapeutic target for osteoporosis.

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“…In this relation, the effects of estrogen on skeletal homeostasis have been studied for bone formation and resorption (seen reviews by PARFITT, 1976b-d). Especially noteworthy is the fact that osteoporosis, a disorder due to excess bone resorption, is known to be more common in postmenopausal women than older men, and estrogens have been implicated in the supression of bone resorptive activity (DUNNILL et al, 1967;HossAIN et al, 1970). Therefore, postmenopausal women may show increased bone wasting, resulting in osteoporosis, and postmenopausal osteoporosis is treatable with estrogen (REIFENSTEIN 1957;HossAIN et al, 1970;GALLAGHER and NORDIN, 1972).…”

Section: Discussionmentioning

confidence: 99%

Sex differences in bone resorption: A scanning electron microscopic study of mouse parietal bones.

Abe

Kanno

Kitao

et al. 1984

Arch. Histol. Jap., Arch. Histol. Jpn

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Summary.Bone resorption surfaces formed by osteoclasts can be identified as rough areas by scanning electron microscopy (SEM). Iu this study, the endocranial surfaces of mouse parietal bones were examined by SEM at 1, 4, 6, 8,14 and 20 weeks of age in an attempt to understand the bone resorbing activity after the cessation of bone growth.On the inner surface of the parietal bones, the rough areas, composed of a group of 8 to 30 ,um wide concavities, can be divided into two distinct groups (type I and type II). The surface of type I areas appeared irregularly rough and those of type II revealed feather, fire-flame, or wave-like patterns due to characteristic arrangements of the concavities. The rough areas occupying the inner surface of the parietal bones made up about 600 of the surface area at 1 week and about 5% at 4 weeks in both sexes.The rough areas were all type I at this age. After puberty, the rough areas in males occupied about 10% of the inner surface until 20 weeks and consisted of large type I and small type II areas.In females, the proportion of the rough areas increased after puberty and occupied about 40% of the endocranial surface at 14 and 20 weeks.The type I areas were much larger, increasing in size with age and eventually occupying a large proportion of the rough areas. The sex differences in the rough areas were reversed by gonadectomy.The results suggest that male and female gonadal products, possibly sex hormones, have respective inhibitory and stimulatory effects on the formation of bone resorptioe surfaces, and may be responsible for the sex differences in the size and morphological features of the areas on the bone surfaces.

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Quantitative histological studies on age changes in bone

Cited by 250 publications

References 16 publications

Correlation between the bone marrow blood perfusion and lipid water content on the lumbar spine in female subjects

Correlation between the bone marrow blood perfusion and lipid water content on the lumbar spine in female subjects

Skeletal Consequences of Deletion of Steroid Receptor Coactivator-2/Transcription Intermediary Factor-2

Sex differences in bone resorption: A scanning electron microscopic study of mouse parietal bones.

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