Quantitative histofluorescence of hippocampal mossy fiber zinc

Savage, Daniel D.; Montano, Christine Y.; Kasarskis, Edward J.

doi:10.1016/0006-8993(89)91073-1

Cited by 40 publications

(22 citation statements)

References 17 publications

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“…The details characterizing this analytical procedure have been de scribed previously [18]. Sixty microliters of TS-Q staining solution [0.003% TS-Q (N-6-methoxy-8-quinolyl-/J-toluenesulfonamide, Al drich Rare Chemicals/Alfred Bader Chemicals, Milwaukee, Wise.)…”

Section: Zinc-ts-q Histofluorescence O F Hippocampal Mossy Fiber Zincmentioning

confidence: 99%

“…It has been estimated that histochemically detectable mossy fiber zinc accounts for 8-15% of total hippocampal zinc [18][19][20]. Given the impact of perinatal hypothyroidism on hippo campal kainate receptors and the similar anatomical loca tion of these kainate receptors and mossy fiber zinc, we hypothesized that perinatal PTU treatment causes hippo campal mossy fiber zinc deficits.…”

mentioning

confidence: 98%

“…and quinoline fluorescence methods [17,18]. It has been estimated that histochemically detectable mossy fiber zinc accounts for 8-15% of total hippocampal zinc [18][19][20].…”

mentioning

confidence: 99%

“…Given the impact of perinatal hypothyroidism on hippo campal kainate receptors and the similar anatomical loca tion of these kainate receptors and mossy fiber zinc, we hypothesized that perinatal PTU treatment causes hippo campal mossy fiber zinc deficits. This hypothesis was tested by measuring the chelatable pool of mossy fiber zinc by quantitative quinoline histofluorescence [18] in untreated control and PTU-treated rats. In addition, tissue zinc and serum zinc concentrations were deter mined by atomic absorption spectroscopy.…”

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confidence: 99%

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Perinatal Hypothyroidism Decreases Hippocampal Mossy Fiber Zinc Density in Rats

Savage

Otero²,

Montano³

et al. 1992

Neuroendocrinology

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The effect of perinatal hypothyroidism on hippocampal mossy fiber zinc density was examined in rats. Timed pregnant Sprague-Dawley rat dams were given water containing either 0.02% propylthiouracil (PTU) or vehicle from gestational day 18 until their litters were weaned on postnatal day 31. Hippocampal mossy fiber zinc density was reduced by 75% in both the dorsal and ventral hippocampal formation CA3 stratum lucidum region of 31-day-old PTU-treated rats compared to untreated controls. Perinatal hypothyroidism did not alter hippocampal tissue zinc concentration, indicating that the PTU-induced reduction in mossy fiber zinc was not a consequence of reduced hippocampal zinc concentration. At 120 days of age, 3 months after discontinuation of PTU treatment, hippocampal mossy fiber zinc density remained significantly reduced by 33-45% in PTU-treated rats compared to control. These data indicate that perinatal hypothyroidism causes a long-lasting reduction in hippocampal mossy fiber zinc density.

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Section: Zinc-ts-q Histofluorescence O F Hippocampal Mossy Fiber Zincmentioning

confidence: 99%

mentioning

confidence: 98%

“…and quinoline fluorescence methods [17,18]. It has been estimated that histochemically detectable mossy fiber zinc accounts for 8-15% of total hippocampal zinc [18][19][20].…”

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Perinatal Hypothyroidism Decreases Hippocampal Mossy Fiber Zinc Density in Rats

Savage

Otero²,

Montano³

et al. 1992

Neuroendocrinology

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“…Therefore, several chemical tools for measuring Zn 2ϩ in living cells have been developed. [8][9][10][11][12][13][14][15][16] Fluorescent probes based on quinoline, fluorescein, other fluorophores or proteins, have been reported. Some of these probes can be used to monitor the change of Zn 2ϩ concentration under physiological conditions, but they suffer from problems such as inadequate selectivity, insufficient sensitivity, and pH-sensitivity.…”

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confidence: 99%

A Novel Fluorescent Probe for Zinc Ion Based on Boron Dipyrromethene (BODIPY) Chromophore

Koutaka¹,

Kosuge²,

Fukasaku³

et al. 2004

CHEMICAL & PHARMACEUTICAL BULLETIN

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Zinc ion (Zn 2ϩ ) is found in every cell in the human body, and is the second most abundant heavy metal ion after iron. It is an essential component of many enzymes and transcription factors (e.g., carbonic anhydrase, zinc finger proteins, etc.).1) In addition to this protein-bound Zn 2ϩ, chelatable Zn 2ϩ is present, especially in the brain, 2) pancreas 3) and spermatozoa.4) Certain neurons in the brain contain a relatively large pool of free Zn 2ϩ sequestered in vesicles in their terminals. Such Zn 2ϩ is released from nerve terminals by excitatory signals, and modulates the function of glutamate receptors. In the pancreas, Zn 2ϩ is co-stored with insulin in secretary vesicles of pancreatic b-cells, and is released when insulin is secreted.5) Zn 2ϩ also suppresses apoptosis, 6) and induces the formation of b-amyloid, 7) which may be related to the etiology of Alzheimer's disease.Although Zn 2ϩ plays many physiologically important roles, the mechanisms involved are still poorly understood. Therefore, several chemical tools for measuring Zn 2ϩ in living cells have been developed. [8][9][10][11][12][13][14][15][16] Fluorescent probes based on quinoline, fluorescein, other fluorophores or proteins, have been reported. Some of these probes can be used to monitor the change of Zn 2ϩ concentration under physiological conditions, but they suffer from problems such as inadequate selectivity, insufficient sensitivity, and pH-sensitivity. We have already developed fluorescein-based probes, ZnAFs, 17,18) which have high selectivity and sensitivity, and ZnAF-Rs, 19) whose structure is based on benzofuran derivatives, and which enable ratiometric measurement. Here we report the design and synthesis of a new fluorescent probe for Zn 2ϩ based on the BODIPY (boron dipyrromethene) chromophore. BODIPY has a high molar extinction coefficient and fluorescence quantum yield. Furthermore, it has the advantages of less sensitivity to solvent polarity and pH than fluorescein-based Zn 2ϩ probes, and its structure can be modified to change its excitation and emission wavelengths. Results and DiscussionProbe Design Based on Photoinduced Electron Transfer Our group has developed fluorescein-based probes for nitric oxide (DAFs), 20,21) for singlet oxygen (DPAXs, DMAXs), 22,23) and for Zn 2ϩ (ZnAFs). As a basis for the design of these probes, we have utilized the photoinduced electron transfer (PeT) between the xanthene ring, which is an electron acceptor and fluorophore, and the benzoic acid moiety, which is an electron donor, and the probes exhibit fluorescence off/on switching that is dependent on the highest occupied molecular orbital (HOMO) level of the benzoic acid moiety. Amino-or oxygen-substituted BODIPYs have already been reported as pH or alkali metal and alkaline earth metal sensors.24-27) However, BODIPY-based functional probes have not yet been developed for biological applications.The fluorescence property of 1,3,5,7-tetramethyl-8-phenyl-BODIPY is thought to be controlled by electron transfer between the phenyl ring at the...

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