Quantitative Hemoglobin Tomography with Diffuse Near-Infrared Spectroscopy: Pilot Results in the Breast

Pogue, Brian W.; Poplack, Steven P.; McBride, Troy O.; Wells, Wendy A.; Osterman, K; Österberg, Ulf L.; Paulsen, Keith D.

doi:10.1148/radiology.218.1.r01ja51261

Cited by 420 publications

(291 citation statements)

References 16 publications

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“…Surface and multispectral images have been used to assess epithelial tissue structure and physiology [1][2][3], brain function [4,5] or superficial redox potential [6]. Probing of hemoglobins deeper in tissue has been used in functional transillumination or reflectance imaging [7,8] or diffuse optical tomography [9][10][11][12][13][14]. Other techniques have exploited light polarization [15] or light interference [16,17].…”

mentioning

confidence: 99%

Shedding light onto live molecular targets

Weissleder

Ntziachristos

2003

Nat Med

1,762

1,304

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Abstract-Optical sensing of specific molecular targets and pathways deep inside living mice has become possible as a result of a number of advances. These include design of biocompatible near-infrared fluorochromes, development of targeted and activatable 'smart' imaging probes, engineered photoproteins and advances in photon migration theory and reconstruction. Together, these advances will provide new tools making it possible to understand more fully the functioning of protein networks, diagnose disease earlier and speed along drug discovery.

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mentioning

confidence: 99%

Shedding light onto live molecular targets

Weissleder

Ntziachristos

2003

Nat Med

1,762

1,304

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“…They found differences in composition between premenopausal and postmenopausal superficial breast tissue and correlations in water and lipid content with age. Pogue et al (9) presented results, again by using spectra from the literature, to evaluate measurements at four or five wavelengths from a frequency-domain system that imaged the whole breast. They reported typical quantitative values for hemoglobin concentration ranging from 10 to 60 M. Shah et al (10) described spectroscopy studies of superficial breast tissue in 14 subjects by using measurements at four wavelengths and showed differences in the physiological properties between premenopausal women, postmenopausal women, and women using hormone replacement therapy, where the hemoglobin content of premenopausal breast tissue was found to be highest relative to subjects with hormone replacement therapy and postmenopausal tissue, which were intermediate and lowest of the three groups, respectively.…”

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confidence: 99%

“…Specifically, a frequency-domain diffuse tomography system recorded intensity and phase shift of light signals through the breast in three contiguous 1-cm sections, where a diffusion model of light transport underpins the estimation of the absorption ( a ) and transport scattering ( Ј s ) coefficients. The absorption coefficient spectra, a ( ), are used to infer the concentrations of hemoglobin, oxyhemoglobin, and water by using the extinction spectra of these constituents, whereas the scattering coefficient spectra, Ј s ( ), are fit with a simplified Mie theory to derive scattering power and amplitude estimates that are related to structural particle size and density in the breast (9,12).…”

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confidence: 99%

Interpreting hemoglobin and water concentration, oxygen saturation, and scattering measured in vivo by near-infrared breast tomography

Srinivasan

Pogue

Jiang

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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Near-infrared spectroscopic tomography was used to measure the properties of 24 mammographically normal breasts to quantify whole-breast absorption and scattering spectra and to evaluate which tissue composition characteristics can be determined from these spectra. The absorption spectrum of breast tissue allows quantification of (i) total hemoglobin concentration, (ii) hemoglobin oxygen saturation, and (iii) water concentration, whereas the scattering spectrum provides information about the size and number density of cellular components and structural matrix elements. These property data were tested for correlation to demographic information, including subject age, body mass index, breast size, and radiographic density. Total hemoglobin concentration correlated inversely to body mass index, likely because lower body mass indicates proportionately less fat and more glandular tissue, and glandular tissue contains greater vascularity, hence, more total hemoglobin. Optical scattering was correlated to breast diameter, subject age, and radiographic density. In the radiographic density, fatty breasts had low scattering power and extremely dense breasts had higher values. This observation is consistent with low attenuation of conventional x-rays with fat and higher attenuation in glandular tissues. Optically, fatty tissues have large scatterers leading to a low scattering power, whereas glandular or fibrous tissues have more cellular and collagen-based structures that lead to high scattering power. The study presents correlative data supporting the hypothesis that optical measurements of absorption and scattering can provide physiologically relevant information about breast tissue composition. These breast constituents vary significantly between individuals and can be altered because of changes in breast physiology or pathological state.T he breast is a highly heterogeneous and dynamically complex organ whose characteristics depend on factors such as age, hormonal status, habitus, family and medical history, and genetics (1-3). Normal breast tissue changes considerably during development, pregnancy, and menopause, and throughout the menstrual cycle. It has been documented that blood flow can increase up to 50% at the time of ovulation; and by the end of the monthly cycle, some women may undergo breast enlargement of up to 20% because of increased vascularity and water content (1). Total blood content can vary up to a factor of 5 or more between women based on their body-fat content, making the breast one of the most physiologically variable tissues in the human body. With nearinfrared (NIR) spectral imaging, hemoglobin concentration, oxygen saturation, and water content can yield information about the current physiological state of the normal breast.The relatively good transparency of tissue in the red and NIR spectrum (i.e., 600-1,000 nm) permits sufficient light penetration to detect signals through as much as a dozen centimeters of the breast. In the NIR spectrum, the primary absorbers of light are hemoglobin, oxyh...

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“…Diffuse optical tomography ͑DOT͒ is a viable new biomedical imaging modality. 36 This technique images the absorption and scattering properties of biological tissues and has been explored as a diagnosis tool in breast, [37][38][39][40][41][42] brain, 43,44 and bones and joints. 45 Some preliminary attempts have been made to perform DOT for prostate.…”

Section: Iib3 Anatomic and Optical Imagingmentioning

confidence: 99%

The role of photodynamic therapy (PDT) physics

2008

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Photodynamic therapy ͑PDT͒ is an emerging treatment modality that employs the photochemical interaction of three components: light, photosensitizer, and oxygen. Tremendous progress has been made in the last 2 decades in new technical development of all components as well as understanding of the biophysical mechanism of PDT. The authors will review the current state of art in PDT research, with an emphasis in PDT physics. They foresee a merge of current separate areas of research in light production and delivery, PDT dosimetry, multimodality imaging, new photosensitizer development, and PDT biology into interdisciplinary combination of two to three areas. Ultimately, they strongly believe that all these categories of research will be linked to develop an integrated model for real-time dosimetry and treatment planning based on biological response.

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Quantitative Hemoglobin Tomography with Diffuse Near-Infrared Spectroscopy: Pilot Results in the Breast

Cited by 420 publications

References 16 publications

Shedding light onto live molecular targets

Shedding light onto live molecular targets

Interpreting hemoglobin and water concentration, oxygen saturation, and scattering measured in vivo by near-infrared breast tomography

The role of photodynamic therapy (PDT) physics

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