Quantitative glycomics monitoring of induced pluripotent- and embryonic stem cells during neuronal differentiation

Terashima, Michiyo; Amano, Maho; Onodera, Tomohiro; Nishimura, Shin‐Ichiro; Iwasaki, Norimasa

doi:10.1016/j.scr.2014.10.006

Cited by 22 publications

(14 citation statements)

References 31 publications

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“…To characterize the changes in TIG-1 cell glycosylation patterns, we performed glycoblotting analysis. Total N -glycans collected from young TIG-1 cells, ATP6V0A2-silenced young TIG-1 cells, old TIG-1 cells, and ATP6V0A2-overexpressing TIG-1 cells were analyzed and identified by a combination of glycoblotting and MALDI-TOF MS 13 14 . Total N -glycan levels increased in old TIG-1 cells ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Total cellular N -glycans were released as described 13 24 with minor modifications. Trypsinized cells were lysed by 10% of Triton X-100 for 50 min on ice and sonicated at room temperature for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…N -glycans were analyzed as described 13 14 . In short, a 250-μL aliquot of BlotGlyco H beads (Sumitomo Bakelite Co., Tokyo, Japan) was reacted with 20 μL PNGase F-digested sample, adjusted to 100 μg protein/20 μL water, with 180 μL of 2% acetic acid in acetonitrile (ACN) at 80 °C for 45 min so that the total glycans were specifically captured by the beads via stable hydrazone bonds.…”

Section: Methodsmentioning

confidence: 99%

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Impaired ATP6V0A2 expression contributes to Golgi dispersion and glycosylation changes in senescent cells

Udono

Fujii

Harada

et al. 2015

Sci Rep

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Many genes and signaling pathways have been found to be involved in cellular senescence program. In the present study, we have identified 16 senescence-associated genes by differential proteomic analysis of the normal human diploid fibroblast cell line, TIG-1, and focused on ATP6V0A2. The aim of this study is to clarify the role of ATP6V0A2, the causal gene for ARCL2, a syndrome of abnormal glycosylation and impaired Golgi trafficking, in cellular senescence program. Here we showed that ATP6V0A2 is critical for cellular senescence; impaired expression of ATP6V0A2 disperses the Golgi structure and triggers senescence, suggesting that ATP6V0A2 mediates these processes. FITC-lectin staining and glycoblotting revealed significantly different glycosylation structures in presenescent (young) and senescent (old) TIG-1 cells; reducing ATP6V0A2 expression in young TIG-1 cells yielded structures similar to those in old TIG-1 cells. Our results suggest that senescence-associated impaired expression of ATP6V0A2 triggers changes in Golgi structure and glycosylation in old TIG-1 cells, which demonstrates a role of ATP6V0A2 in cellular senescence program.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Impaired ATP6V0A2 expression contributes to Golgi dispersion and glycosylation changes in senescent cells

Udono

Fujii

Harada

et al. 2015

Sci Rep

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“…To improve the effectiveness of neural-inductive moieties and promote iPSC neurogenesis, biomaterials can be chemically enhanced. For example, synthetic neurotransmitter analogs have been added to promote neuronal-fate differentiation of iPSCs [240] [241],. Promising practical advances include biomaterials that can control the presentation of neuroninductive growth factors in a sustained fashion.…”

Section: Risksmentioning

confidence: 99%

Advances in Pluripotent Stem Cells: History, Mechanisms, Technologies, and Applications

Liu

David

Trawczynski

et al. 2019

Stem Cell Rev and Rep

353

209

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Over the past 20 years, and particularly in the last decade, significant developmental milestones have driven basic, translational, and clinical advances in the field of stem cell and regenerative medicine. In this article, we provide a systemic overview of the major recent discoveries in this exciting and rapidly developing field. We begin by discussing experimental advances in the generation and differentiation of pluripotent stem cells (PSCs), next moving to the maintenance of stem cells in different culture types, and finishing with a discussion of three-dimensional (3D) cell technology and future stem cell applications. Specifically, we highlight the following crucial domains: 1) sources of pluripotent cells; 2) next-generation in vivo direct reprogramming technology; 3) cell types derived from PSCs and the influence of genetic memory; 4) induction of pluripotency with genomic modifications; 5) construction of vectors with reprogramming factor combinations; 6) enhancing pluripotency with small molecules and genetic signaling pathways; 7) induction of cell reprogramming by RNA signaling; 8) induction and enhancement of pluripotency with chemicals; 9) maintenance of pluripotency and genomic stability in induced pluripotent stem cells (iPSCs); 10) feeder-free and xenon-free culture environments; 11) biomaterial applications in stem cell biology; 12) three-dimensional (3D) cell technology; 13) 3D bioprinting; 14) downstream stem cell applications; and 15) current ethical issues in stem cell and regenerative medicine. This review, encompassing the fundamental concepts of regenerative medicine, is intended to provide a comprehensive portrait of important progress in stem cell research and development. Innovative technologies and real-world applications are emphasized for readers interested in the exciting, promising, and challenging field of stem cells and those seeking guidance in planning future research direction.

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“…To further improve the differentiation efficiency compared with using soluble inductive moieties alone, biomaterials can be chemically modified to display neural‐inductive moieties. For example, the addition of synthetic neurotransmitter analogs has led to enhanced neuronal differentiation of iPSCs (Terashima et al , ; Zhang et al , ). One can envision that immobilizing these moieties and enhancing their local availability may trigger relevant signaling cascades and promote the neuronal differentiation of iPSCs in a more robust manner (Kuo & Chung, ; Kuo & Wang, ; Kuo & Chang, ; Lei & Schaffer, ).…”

Section: Advanced Biomaterials For the Directed Differentiation Of Ipmentioning

confidence: 99%

Application of biomaterials to advance induced pluripotent stem cell research and therapy

et al. 2015

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Derived from any somatic cell type and possessing unlimited selfrenewal and differentiation potential, induced pluripotent stem cells (iPSCs) are poised to revolutionize stem cell biology and regenerative medicine research, bringing unprecedented opportunities for treating debilitating human diseases. To overcome the limitations associated with safety, efficiency, and scalability of traditional iPSC derivation, expansion, and differentiation protocols, biomaterials have recently been considered. Beyond addressing these limitations, the integration of biomaterials with existing iPSC culture platforms could offer additional opportunities to better probe the biology and control the behavior of iPSCs or their progeny in vitro and in vivo. Herein, we discuss the impact of biomaterials on the iPSC field, from derivation to tissue regeneration and modeling. Although still exploratory, we envision the emerging combination of biomaterials and iPSCs will be critical in the successful application of iPSCs and their progeny for research and clinical translation.

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Quantitative glycomics monitoring of induced pluripotent- and embryonic stem cells during neuronal differentiation

Cited by 22 publications

References 31 publications

Impaired ATP6V0A2 expression contributes to Golgi dispersion and glycosylation changes in senescent cells

Impaired ATP6V0A2 expression contributes to Golgi dispersion and glycosylation changes in senescent cells

Advances in Pluripotent Stem Cells: History, Mechanisms, Technologies, and Applications

Application of biomaterials to advance induced pluripotent stem cell research and therapy

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