Quantitative expression of myogenic regulatory factors MyoD and myogenin in pacu (Piaractus mesopotamicus) skeletal muscle during growth

Almeida, Fernanda Losi Alves de; Pessotti, Nabila Scabine; Pinhal, Danillo; Padovani, Carlos Roberto; Leitão, Natália de Jesus; Carvalho, Robson Francisco; Martins, Cesar; Portella, Maria Célia; Dal-Pai-Silva, Maeli

doi:10.1016/j.micron.2010.06.012

Cited by 53 publications

(45 citation statements)

References 48 publications

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“…These morphological characteristics are consistent with those reported for other fish species whose white muscle mass is of economic importance (Fernandez et al, 2000;Dal Pai-Silva et al, 2003a,b;Aguiar et al, 2005;de Almeida et al, 2008de Almeida et al, , 2010. Moreover, mosaic hyperplasia was ubiquitous at detected stages, as previously reported in other species (Rowlerson and Veggetti, 2001;de Almeida et al, 2008).…”

Section: Morphological Analysissupporting

confidence: 91%

“…The highest and lowest frequencies of fiber of <20 µm diameter occurred in the white muscle of 120 dph and adult fish, respectively. The presence of fibers of <20 µm diameter indicated active hyperplastic growth, which clearly contributed to M. amblycephala skeletal muscle growth at 120 dph (Valente et al, 1999;Rowlerson and Veggetti, 2001;de Almeida et al, 2010). Our study showed that muscle fiber recruitment in adults was lower than during juvenile development (60-180 dph).…”

Section: Morphological Analysismentioning

confidence: 54%

“…Our study showed that muscle fiber recruitment in adults was lower than during juvenile development (60-180 dph). The lower frequency of fibers of >50 µm diameter at 120 dph indicated that muscle fiber hypertrophy was less prominent at this stage (de Almeida et al, 2008(de Almeida et al, , 2010; the highest rate of fibers of >50 µm diameter occurred at 180 dph and in adults suggesting that muscle fiber hypertrophy was occurring (Valente et al, 1999;Rowlerson and Veggetti, 2001;de Almeida et al, 2010).…”

Section: Morphological Analysismentioning

confidence: 99%

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Morphological analysis and muscle-associated gene expression during different muscle growth phases of Megalobrama amblycephala

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Skeletal muscle growth is regulated by both positive and negative factors, such as myogenic regulatory factors (MRFs) and myostatin (MSTN), and involves both hyperplasia and hypertrophy. In the present study, morphological changes during muscle development in Megalobrama amblycephala were characterized and gene expression levels were measured by quantitative real-time polymerase chain reaction (qRT-PCR) analysis in juvenile [60, 90, 120, and 180 days post-hatching (dph)] and adult fish. Our results show that during muscle development, the frequency of muscle fibers with a diameter <20 µm dramatically decreased in both red and white muscles, with a concomitant increase in the frequency of >30 µm fibers in red muscle and >50 µm fibers in (2015) white muscle. At 90-120 dph, the ratio of hyperplastic to hypertrophic areas in red and white muscles increased, but later decreased at 120-180 dph. The effect of hypertrophy was significantly larger than hyperplasia during these phases. qRT-PCR indicated MRF and MSTN (MSTNa and MSTNb) genes had similar expression patterns that peaked at 120 dph, with the exception of MSTNa. This new information on the molecular regulation of muscle growth and rapid growth phases will be of value to the cultivation of M. amblycephala.

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Section: Morphological Analysissupporting

confidence: 91%

Section: Morphological Analysismentioning

confidence: 54%

Section: Morphological Analysismentioning

confidence: 99%

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Morphological analysis and muscle-associated gene expression during different muscle growth phases of Megalobrama amblycephala

et al. 2015

Genet. Mol. Res.

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“…The process of hyperplasia is predominant in the initial life stages of animals (Almeida et al, 2010); nevertheless, as indicated in the present study, factors such as nutrition may affect the dynamics of muscle fiber growth. With the increased FPH inclusion corresponding to the post-larvae mass gain, there was a frequency decrease in the c10 class muscle fiber and a consequent increase in the c20 class, that is, there was a negative correlation between mass gain and class <10 (−0.64), and a positive correlation for class c20 (0.62).…”

Section: Resultsmentioning

confidence: 51%

Fish protein hydrolysate in diets for Nile tilapia post-larvae

Silva

Rocha

Moreira

et al. 2017

Pesq. agropec. bras.

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-The objective of this work was to determine the apparent digestibility coefficient (ADC) of crude protein, crude energy, fat, and dry matter of fish protein hydrolysate (FPH), made of by-products of Nile tilapia (Oreochromis niloticus) and whole sardines (Cetengraulis edentulus), and to evaluate the productive performance and muscle fiber growth of Nile tilapia post-larvae. Two trials were conducted, the first one to determine the digestibility in 120 fingerlings (70.0±2.0 g), and the second one to evaluate the productive performance of 375 post-larvae, with three days of age, which were distributed in 25 aquaria with 30 L of useful volume. Five diets were prepared based on vegetable ingredients, to which fish were included at 0, 2, 4, 6, and 8% FPH. For the evaluation of muscle growth, eight fish of each experimental unit were used. The ADC values found were: 98.29% for dry matter; 99.28% for crude protein; and 99.13% for gross energy. The best zootechnical response for the productive performance resulted from the treatment with the inclusion of fish hydrolysate at 4.75%. The diets affected the frequency of the muscle fiber diameters, mainly the growth by hyperplasia. FPH can be efficiently used, and its inclusion at 4.75% is indicated in the diets for Nile tilapia in the post-larvae stage.Index terms: Cetengraulis edentulus, Oreochromis niloticus, digestibility, fish meal, nutrition, peptides, pisciculture. Hidrolisado proteico de peixe em dietas para pós-larvas de tilápia-do-niloResumo -O objetivo deste trabalho foi determinar o coeficiente de digestibilidade aparente (CDA) da proteína bruta, da energia bruta, da gordura e da matéria seca de um hidrolisado proteico de peixe (HPP), feito de resíduos de tilápia-do-nilo (Oreochromis niloticus) e sardinha inteira (Cetengraulis edentulus), e avaliar o desempenho produtivo e o crescimento das fibras musculares de pós-larvas de tilápia-do-nilo. Realizaram-se dois ensaios, o primeiro para a determinação da digestibilidade em 120 alevinos (70,0±2,0 g) e, o segundo, para avaliação do desempenho produtivo de 375 pós-larvas, com três dias de idade, distribuídas em 25 aquários (unidade experimental) com volume útil de 30 L. Cinco rações à base de ingredientes vegetais foram elaboradas, às quais se incluíram os peixes a 0, 2, 4, 6 e 8% de HPP. Para a avaliação do crescimento muscular, oito peixes de cada unidade experimental foram utilizados. Os valores de CDA encontrados foram: 98,29% para matéria seca; 99,28% para proteína bruta; e 99,13% para energia bruta. As melhores respostas zootécnicas quanto ao desempenho produtivo resultaram do tratamento com a inclusão do hidrolisado proteico a 4,75%. As dietas influenciaram a frequência do diâmetro das fibras musculares, principalmente o crescimento por hiperplasia. O HPP pode ser eficientemente utilizado, e sua inclusão a 4,75% é indicada em dietas de tilápia-do-nilo na fase de pós-larva.Termos para indexação: Cetengraulis edentulus, Oreochromis niloticus, digestibilidade, farinha de peixe, nutrição, peptídeos, pisci...

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“…The amplification and dissociation curves, that were generated using version 4.0 of the 7300 System/Sequence Detection software program (Applied Biosystems), were used for gene expression analysis. The qRT-PCR signals were normalized to a segment of the 18S rRNA housekeeping gene using the primers 18S3 (5'-CGGAATGAGCGTATCCTAAACC-3') and 18S4 (5'-GCTGCTGGCACCAGACTTG-3'), also designed based on consensus sequences of this gene described for L. macrocephalus and other fish species (Tom et al 2004, Alves-Costa and Wasko 2010, Almeida et al 2010.…”

Section: Rt-pcr and Cdna Amplificationmentioning

confidence: 99%

Characterization of α-actin isoforms in white and red skeletal muscle types of Leporinus macrocephalus (Characiformes, Anostomidae)

Alves-Costa

Silva

Wasko

2015

An. Acad. Bras. Ciênc.

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Two α-actin genes of the fish Leporinus macrocephalus, referring to white and red muscle tissues, were isolated. Actin isoforms, that mainly differed by a Ser/Ala 155 substitution, can have a functional significance related to actin-ATP interaction. An Ala 155 residue, as observed in the α-skeletal actin from red muscle, results in a decrease in actin's affinity for ATP, which may also be associated to the slow contractile performance of this tissue. Furthermore, a Phe/Ile 262 substitution at the red muscle actin leads to a hydrophobicity variation at the D-plug of the protein, which could alter its stability. Data on qRT-PCR evidenced a significant higher actin mRNA level in white muscle when compared to red muscle (T=105 Mann Whitney; p<0.001). This finding could be related to the energetic demands of the white muscle tissue, with fast contraction fibers and glycolytic metabolism for energy supply. Available data on muscle actins lead to the proposal that white and red α-skeletal actins are genetically and functionally distinguishable in fish species, a feature that is not found in other vertebrate groups.

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Quantitative expression of myogenic regulatory factors MyoD and myogenin in pacu (Piaractus mesopotamicus) skeletal muscle during growth

Cited by 53 publications

References 48 publications

Morphological analysis and muscle-associated gene expression during different muscle growth phases of Megalobrama amblycephala

Morphological analysis and muscle-associated gene expression during different muscle growth phases of Megalobrama amblycephala

Fish protein hydrolysate in diets for Nile tilapia post-larvae

Characterization of α-actin isoforms in white and red skeletal muscle types of Leporinus macrocephalus (Characiformes, Anostomidae)

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