Quantitative Expression and Immunohistochemical Detection of Glucose Transporters, GLUT1 and GLUT3 in the Rabbit Placenta during Successful Pregnancy

Khan, Hamayun; Kusakabe, Ken Takeshi; Wakitani, Shoichi; Hiyama, Masato; Kiso, Yasuo

doi:10.1292/jvms.11-0144

Cited by 9 publications

(7 citation statements)

References 45 publications

(66 reference statements)

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“…In an experiment on rabbits [7] immunohistochemistry indicated the distribution of GLUT-1 was observed mainly to the surface of the outer trophoblasts, whereas GLUT-3 mainly localized to the basal site of the inner trophoblasts and fetal blood vessels, suggesting that glucose is transported through GLUT-1 from the maternal blood stream for use as a placental fuel and for further transport through GLUT-3 to the fetal circulation. According to the data maximal GLUT-1 syncytial expression is seen near term [8,9].…”

Section: Discussionmentioning

confidence: 98%

“…Conflicting information regarding expression of GLUT-3 protein in the human placenta has been reported and the localization and pattern of expression of GLUT-3 protein across gestation has not been clearly defined. Some data suggest that GLUT-3 expression in term and near term pregnancy placenta is absent in syncytiotrophoblast, other-that that it is localized mainly in the syncytial basal membrane [7,12,13]. Recent study reports that immunohistochemistry localizes GLUT-3 expression to the syncytiotrophoblast although there is also expression in the cytotrophoblast and to some degree in the endothelium.…”

Section: Discussionmentioning

confidence: 99%

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Glucose transporters expression in the placental terminal villi of preeclampsia and intrauterine growth retardation complicated pregnancies

Дубова¹,

Павлов²,

Kulikova³

et al. 2013

Health

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Introduction: Transmembrane sodium-independent glucose transporters (GLUTs) play an important role in both placental and fetal development by the providing transplacental glucose transport. Abnormal GLUT-1 and GLUT-3 placental expression could play a role in such late pregnancy complications development as preeclampsia and intrauterine growth retardation. Materials and Methods: Immunohistochemistry was performed to reveal patterns of GLUT-1 and GLUT-3 expression in the placental terminal villi compartments. Results: GLUT-1 syncytial expression in the terminal villi of severe PE cases (both with and without IUGR) was significantly lower in compare to control group. We also report about GLUT-3 both syncytial and endothelial expression in the near term and term placental terminal villi without significant difference between the study and control groups. Conclusion: other study should be performed to establish the nature of GLUT-1 downregulation in severe preeclampsia cases and whether GLUT-3 is expressed in the syncytiotrophoblast of near term and term placentas or not.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Glucose transporters expression in the placental terminal villi of preeclampsia and intrauterine growth retardation complicated pregnancies

Дубова¹,

Павлов²,

Kulikova³

et al. 2013

Health

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“…Glucose transporters (GLUTs) have been detected at least as early as GD13 in the rabbit placenta and show specific and dynamic changes during gestation. GLUT3 appears to be most abundant on GD13 and is localized to the basal site of the inner trophoblast layer and fetal blood vessels of the labyrinthine zone (Khan, Kusakabe, Wakitani, Hiyama, & Kiso, ). GLUT1 shows similar expression between GD13 and GD28 and is highly expressed in the trophoblast layer of the labyrinthine zone that is in contact with maternal blood (Khan et al, ).…”

Section: Ontogeny Of Placental and Fetal Growth In Rabbitsmentioning

confidence: 99%

Models of Intrauterine growth restriction and fetal programming in rabbits

López-Tello

Álvarez

González-Bulnes³

et al. 2019

Molecular Reproduction Devel

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Intrauterine growth restriction (IUGR) affects approximately 10% of human pregnancies globally and has immediate and life‐long consequences for offspring health. However, the mechanisms underlying the pathogenesis of IUGR and its association with later health and disease outcomes are poorly understood. To address these knowledge gaps, the use of experimental animals is critically important. Since the 50's different environmental, pharmacological, and surgical manipulations have been performed in the rabbit to improve our knowledge of the control of fetal growth, fetal responses to IUGR, and mechanisms by which offspring may be programmed by an adverse gestational environment. The purpose of this review is therefore to summarize the utility of the rabbit as a model for IUGR research. It first summarizes the knowledge of prenatal and postnatal development in the rabbit and how these events relate to developmental milestones in humans. It then describes the methods used to induce IUGR in rabbits and the knowledge gained about the mechanisms determining prenatal and postnatal outcomes of the offspring. Finally, it discusses the application of state of the art approaches in the rabbit, including high‐resolution ultrasound, magnetic resonance imaging, and gene targeting, to gain a deeper integrative understanding of the physiological and molecular events governing the development of IUGR. Overall, we hope to engage and inspire investigators to employ the rabbit as a model organism when studying pregnancy physiology so that we may advance our understanding of mechanisms underlying IUGR and its consequences in humans and other mammalian species.

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“…Blood glucose levels undergo changes during fetal development, it decreases once the pregnancy progresses due to increased absorption of glucose by the placenta and inhibition of gluconeogenesis [35]. Another cause for intensified ketogenesis is the action of placental hormones on maternal hepatocytes.…”

Section: Resultsmentioning

confidence: 99%

Researches on Blood Biochemical Variations During Pregnancy in Rabbits

Lazăr¹,

Vintu²,

P³

et al. 2019

Rev. Chim.

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The organism as a biological system, for the normal performance of its functions, needs a wide variety of substances that it needs to obtain from the environment. Their assurance must be rhythmic and balanced in qualitative and quantitative terms. By its chemical composition, the blood plasma transports the energetic and plastic substances from the forming organs to the cells and tissues, but also the products resulting from the cellular and tissue catabolism of the excretory organs. The research was carried out on the Meuse Rabbit by the Belgian Giant, divided into three experimental NP groups (adult, non-pregnant females), P1 (females at 15 days of gestation) and P2 (females at the end of gestation, 30 days). The collection of blood samples was performed with the vacuum system before meals. Subsequently, they were centrifuged at 3000 rpm for 5 minutes to obtain the plasma required for the biochemical examination. Biochemical parameters were determined by spectophotometry using the ACCENT 200. As a result, we observed that gestation is a diabetogenic stress, the fetus uses glucose almost exclusively as an energy source and increases protein needs due to the permanent transfer of amino acids to the fetus. Lipid metabolism is diverted with priority to lipid consumption, about 20% of free circulating fatty acids are transferred to the fetus.

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Quantitative Expression and Immunohistochemical Detection of Glucose Transporters, GLUT1 and GLUT3 in the Rabbit Placenta during Successful Pregnancy

Cited by 9 publications

References 45 publications

Glucose transporters expression in the placental terminal villi of preeclampsia and intrauterine growth retardation complicated pregnancies

Glucose transporters expression in the placental terminal villi of preeclampsia and intrauterine growth retardation complicated pregnancies

Models of Intrauterine growth restriction and fetal programming in rabbits

Researches on Blood Biochemical Variations During Pregnancy in Rabbits

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