Quantitative expression analysis of nacreous shell matrix protein genes in the process of pearl biogenesis

Wang, Ning; Kinoshita, Shigeharu; Riho, Chihiro; Maeyama, Kaoru; Nagai, Kiyohito; Watabe, Shugo

doi:10.1016/j.cbpb.2009.07.012

Cited by 42 publications

(36 citation statements)

References 28 publications

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“…The expression of each matrix protein gene is restricted to epithelial cells in specific parts of a mantle tissue (Takeuchi and Endo 2005). Differences have been reported in expression patterns of these genes encoding several shell matrix proteins between mantle and pearlsac epithelial cells, and among pearl-sac epithelial cells forming different types of pearls (Takeuchi and Endo 2005;Wang et al 2009;Inoue et al 2010Inoue et al , 2011aInoue et al , 2011b. Daily oscillation in the gene expression is also reported (Miyazaki et al 2008).…”

Section: -4 Relationships Between Morphological Characteristics Of mentioning

confidence: 90%

Fundamental Studies oninvivoandinvitroPearl Formation—Contribution of Outer Epithelial Cells of Pearl Oyster Mantle and Pearl Sacs

Awaji¹,

Machii²

2011

Aqua-BioSci. Monogr. (ABSM)

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is the pallial line, which is an impression of the pallial muscles lined in an arc in the middle of a mantle. Several types of epithelial cells are recognized in the outer epithelium of a pearl oyster mantle (Tsujii 1960;Wada 1966). The most abundant type is named as outer epithelial cells. They are regarded to play a major role in shell formation and regeneration, and their functions are a main theme of this article.A shell of pearl oyster Pinctada fucata, characterized by its beautiful luster over the inner surface, consists of two layers: periostracum and ostracum. The periostracum is a thin, pliable proteinaceous sheet covering the outer surface of the shell. Components of the periostracum are synthesized in a periostracum gland located at the bottom of a periostracal groove between an outer and a middle fold of a mantle. The periostracum is thought to serve as a barrier that iso- Fundamental Studies on in vivo and in vitro AbstractOuter epithelial cells, which constitute a monolayer epithelium covering the outer surface of pearl oyster mantle, play principal roles in shell and pearl formation. In pearl culture, a fragment of the mantle prepared from a donor is implanted into the recipient's gonad together with a small inorganic bead. Histological studies using pearl oyster Pinctada fucata have revealed that the outer epithelial cells emigrate from the allograft, proliferate, and form a pearl sac surrounding the bead. Following the pearl-sac formation, the pearl-sac epithelia start to form calcium carbonate crystals, such as nacre, on the bead showing morphological characteristics closely related with the crystal structures.To investigate cellular mechanisms of the pearl formation, organ and cell culture methods for the outer epithelial cells of pearl oyster mantle were developed. In the organ culture, crystal formation, deposition of shell matrix-like structure, and DNA synthesis of the outer epithelial cells were observed. The outer epithelial cells separated from the mantle started DNA synthesis in co-culture with hemocytes that revealed a part of cellto-cell interactions during the pearl-sac formation processes. Substitution of the cultured outer epithelial cells for a mantle allograft in pearl culture was tested by injection of the cultured cells; the results of which implied future possibilities for the application of the cultured outer epithelial cells for pearl production.

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Section: -4 Relationships Between Morphological Characteristics Of mentioning

confidence: 90%

Fundamental Studies oninvivoandinvitroPearl Formation—Contribution of Outer Epithelial Cells of Pearl Oyster Mantle and Pearl Sacs

Awaji¹,

Machii²

2011

Aqua-BioSci. Monogr. (ABSM)

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“…It indicates existence of a conserved N16 family in Pteriidae and there seems to be evidence in support of this assumption. There have been various reports regarding its functional or expressional analyses (Collino and Evans, 2008;Inoue et al, 2010Inoue et al, , 2011Kinoshita et al, 2011;Montagnani et al, 2011;Takeuchi and Endo, 2006;Wang et al, 2009). Despite its critical role in shell formation, analyses of N16 have been confined to its cDNA.…”

Section: Introductionmentioning

confidence: 98%

Genetic structure and polymorphisms of the N16 gene in Pinctada fucata

Nogawa

Baba

Masaoka

et al. 2012

Gene

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“…Growth inhibition of undesired aragonite crystal faces is an effective way to modify the morphology of CaCO 3 crystals (Samata et al, 1999;Wang et al, 2009;Kinoshita et al, 2011;Masaoka et al, 2013). We found that the matrix proteins mRNAs showed a similar transcription pattern to Pf-YY-1.…”

Section: Accepted Manuscriptmentioning

confidence: 95%

Cloning and identification of a YY-1 homolog as a potential transcription factor from Pinctada fucata

Zheng

Cheng

Xiang

et al. 2015

Gene

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Quantitative expression analysis of nacreous shell matrix protein genes in the process of pearl biogenesis

Cited by 42 publications

References 28 publications

Fundamental Studies oninvivoandinvitroPearl Formation—Contribution of Outer Epithelial Cells of Pearl Oyster Mantle and Pearl Sacs

Fundamental Studies oninvivoandinvitroPearl Formation—Contribution of Outer Epithelial Cells of Pearl Oyster Mantle and Pearl Sacs

Genetic structure and polymorphisms of the N16 gene in Pinctada fucata

Cloning and identification of a YY-1 homolog as a potential transcription factor from Pinctada fucata

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