Quantitative Evaluation of Vertebral Microvascular Permeability and Fat Fraction in Alloxan-induced Diabetic Rabbits

Abstract

Purpose To determine longitudinal relationships between lumbar vertebral bone marrow permeability and marrow adipose tissue in a rabbit diabetes model by using quantitative dynamic contrast agent-enhanced (DCE) magnetic resonance (MR) imaging and iterative decomposition of water and fat with the echo asymmetry and least-squares estimation quantitation (IDEAL IQ) sequence. Materials and Methods Twenty rabbits were randomly assigned to the diabetic (n = 10) or control (n = 10) group. All rabbits underwent sagittal MR imaging of the lumbar region at fixed time points (0, 4, 8, 12, and 16 weeks after alloxan injection). A linear mixed-effects model was used to analyze fat fraction (FF) and permeability parameter changes for 16 months after baseline. These parameters were compared between the two groups by using an independent-samples t test. Correlation of DCE MR imaging parameters with FF and with microvessel density (MVD) was analyzed by using the Spearman correlation coefficient. All statistical analyses were performed with software. Results Twelve weeks after injection, transfer constant (K) and rate constant (K) were markedly and significantly increased, while fractional plasma volume (V) significantly decreased. The volume of extravascular extracellular space (V) decreased significantly after 16 weeks in the diabetic group. MVD was negatively correlated with K and K and positively correlated with V and V, while FF was positively correlated with K and K and negatively correlated with V and V (P < .05 for all). Conclusion DCE MR imaging and the IDEAL IQ sequence can be used for quantitative evaluation of changes in vertebral microvascular permeability and vertebral fat deposition in alloxan-induced diabetic rabbits. This variation is highly associated with increased vertebral fat deposition. RSNA, 2017 Online supplemental material is available for this article.

Quantification of pancreatic proton density fat fraction in diabetic pigs using MR imaging and IDEAL-IQ sequence

“…Prior studies have shown that biopsy is difficult and many complications are involved in assessment of diabetic pancreatic pathology; such studies often involve the use of human organ donor tissue samples [24, 25] or the establishment of animal models [26, 27]. Hu et al found that the IDEAL IQ sequence can be used for quantitative evaluation of vertebral fat deposition in alloxan-induced diabetic rabbits; however, changes in PFC remain unclear in the context of diabetic rabbits [28]. Importantly, unlike previous MRI techniques used to evaluate visceral fat, the IDEAL-IQ enables adjustment for common biases in the measurement of tissue fat, including T 1 and T 2 * effects, as well as main magnetic field inhomogeneity [29, 30].…”

Radiomics Analysis of Multiparametric MRI Evaluates the Pathological Features of Cervical Squamous Cell Carcinoma

“…China) to generate permeability parameter maps. The Omni‐Kinetics software was designed to generate quantitative parameter maps of DCE and provided not only conventional mean value but also various radiomics features . We applied the extended Tofts linear pharmacokinetic model for DCE‐MRI data analysis.…”

Magnetic resonance imaging: Proton density fat fraction for assessment of pancreatic fatty infiltration during progression of T2DM bama minipigs

“…This technique is often used to quantify hepatic fat in patients with nonalcoholic fatty liver disease . Hu et al reported that the IDEAL‐IQ sequence was useful for quantitative evaluation of changes in vertebral fat deposition in alloxan‐induced diabetic rabbits. Here, we used the IDEAL‐IQ sequence to measure PFF, HFF, and R 2 * values; our results showed that PFF and PFC had a strong positive correlation.…”