Quantitative evaluation of the oxidation of an exogenous glucose load using naturally labeled 13C-glucose

Mosora, F.; Lefèbvre, Pierre; Pirnay, F; Lacroix, M.; Luyckx, A; Duchesne, Jean-Clair

doi:10.1016/0026-0495(76)90110-4

Cited by 67 publications

(47 citation statements)

References 13 publications

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“…The % dose recovered per hour at various times and the cum % dose recovered (area under the curve) for all infants studied with [I3C]-1-L are shown in Table 2. The data are remarkably similar to those previously reported by Mosora et al (16) in adults studied after a load of 95 g of maize glucose, whose natural abundance of [I3c] is enriched by approximately 1%, thus allowing its use as a natural label (19). The % dose per hour and the cum % dose recovered did not differ between the eight infants excreting significant amounts of hydrogen and those who were not hydrogen excretors.…”

Section: Breath ['3c0~] After ['3c]-i-l and [13c]-i-g ['3c0~supporting

confidence: 79%

Lactose Assimilation by Full-Term Infants: Relation of [13C] and H2 Breath Tests with Fecal [13C] Excretion

et al. 1983

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Neutral P-galactosidase (lactase) located in the bush border of adults varied from 0.950-0.997; the ratio of the cumulative % dose the small intestinal enterocyte is the enzyme responsible for the [13Cj (cum % [13Cj) to the cumulative % dose [14cj (cum % [14cj) hydrolysis of lactose, which is the rate limiting step in lactose ranged from 0.89-1.15. Nineteen full-term infants (40.1 + 0.9 wk absorption (8, 11). Lactase, unlike sucrase and maltase, remains gestation) were subsequently studied. On day 1 baseline breath low in concentration during fetal development until approximately and stool collections were carried out with unlabeled substrate 36 wk of gestation and then increases continuously thereafter until (lactose-containing formula). In the initial ten infants [13Cj-1-L term (1, 10). Normal full-term infants, whose only natural dietary was added to the 7 a.m. or 8 a.m. formula feeding on day 2 and an carbohydrate is lactose, have always been considered "by defini&h breath test was done. A 24-h stool collection was also carried tion" to be lactose tolerant. Implicit in this belief have been the out. In nine subsequent infants a breath test and stool collection concepts that intestinal lactase is wholly responsible for lactose with [13Cj-l-glucose ([13CJ-1-G) were carried out on day 2 and the digestion and that the entire process of digestion and absorption studies with [13CJ-1-L were moved to day 3. Hz and % d [13Cj/h takes place in the small intestine. and cum % [13CJ in breath and [l3Cj recovered in stool were Auricchio and his collaborators (2) were among the first to determined. question these concepts. Based on in vitro studies with material All infants had detectable Hz in breath on the days of study. obtained at autopsy they concluded that the "large lactose load of Peak Hz excretion was <20 ppm in eleven infants. 220 ppm in the breast-fed (full-term) infant probably exceeds the ability of the eight. There was no relationship of HZ excretion to gestational small intestine to hydrolyze this sugar" (2). Both the low pH of age, birth weight, postnatal age or lactose intake. In all 19 infants stool and the fmding of significant (21%) amounts of reducing the % d [13cj in breath per h and the cum % [13Cj after [13C]-1-L substance in the stool of healthy term infants corroborate this idea were virtually identical to data in adults studies after consumption and suggest that carbohydrate normally passes beyond the ileoceof naturally [13C]-labeled maize glucose. In the infants studied cal valve. The excretion of hydrogen in breath in substantial with both substrates, there were no significant differences between amounts implicates the colonic microflora in anaerobic fermen-[l3Cj-1-~ and [l3CJ-1-G i, % d [13Cj at each time point. Cum % tation of this malabsorbed carbohydrate (3, 6). In addition to [13C] recovered from [13Cj-1-G become progressively greater than hydrogen gas, volatile fatty acids are produced. These compounds that from [13C]-1-L, significantly so at 6, 7, and 8 h (8 hour % d have been shown to be...

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Section: Breath ['3c0~] After ['3c]-i-l and [13c]-i-g ['3c0~supporting

confidence: 79%

Lactose Assimilation by Full-Term Infants: Relation of [13C] and H2 Breath Tests with Fecal [13C] Excretion

et al. 1983

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“…The external precision of breath CO 2 measurements (n 5) for the mass spectrometer used was AE 0.120d. Exogenous (ingested) fat oxidation was estimated according to Mosora et al (1976) using the equation shown below. For a fuller description of the assumptions and derivation of the equation see Sonko (1992).…”

Section: Calorimetry Techniques and Precisionmentioning

confidence: 99%

Dose-response relationship between fat ingestion and oxidation: quantitative estimation using whole-body calorimetry and 13C isotope ratio mass spectrometry

Coward

et al. 2001

Eur J Clin Nutr

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Objective: To determine dose-dependent relationship between ingested fat and its oxidation in the immediate post-prandial period in humans. Design: Subjects were randomly selected for the study at the Dunn Clinical Nutrition Centre, Cambridge, UK. Subjects ingested naturally enriched 13 C corn-oil doses (range 20 ± 140 g) in a whole-body indirect calorimeter, and were studied for 8 h. Ingested fat oxidation was estimated from the subject's breath 13 C enrichment and total carbon dioxide production. Total fat and carbohydrate oxidation were estimated from non-protein oxygen and carbon dioxide exchanges. Endogenous fat oxidation was estimated as the difference between total fat and ingested fat oxidation. Results: The amount of fat dose oxidized was nonlinearly related to the amount ingested. On average, 25.6 AE 2.7% of the mean fat dose was oxidized. A signi®cant (r 7 0.72, P`0.001) inverse correlation was found between the amount of fat dose and the proportion oxidized. Endogenous carbohydrate oxidation was negatively and signi®cantly correlated to fat dose oxidized (r 7 0.61, P`0.01), but it was not correlated to endogenous fat oxidation.Conclusions: There was a nonlinear relationship between amount of fat dose and its quantity that was oxidized in the immediate post-prandial period. The inverse relationship between the size of the fat load and the proportion that was oxidized post-prandially implies increased dietary fat storage beyond about 50 g in a normal resting adult. This has important implications for 13 CO 2 -based studies.

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“…Après avoir incorrectement attribué ces variations à des effets isotopiques différents d'une espèce à l'autre , ils s'aperçoivent qu'elles sont simplement dues à la variation de 13 C/ 12 C dans la nourriture ingérée Mosora et al, 1971 Figure 2.9), une observation compatible avec un retard de l'entrée du glucose dans les cellules (Duchesne et al, 1973). Suite à cette série d'expériences, Mosora et al (1976) rapportent l'oxydation d'une charge de glucose naturellement enrichi en 13 C au repos chez huit sujets sains (Table 2.III). On a aujourd'hui des raisons de penser que ces données surestiment l'oxydation réelle du glucose exogène en raison de la perte de 13 C dans la voie de synthèse indirecte du glycogène (Folch et al, 2005).…”

Section: Développement Du Traçage Au 13 Cunclassified

Sélection des substrats lors de l’exercice prolongé avec ingestion de glucides : études par traçage au ¹³C

Tremblay¹

2011

Appl. Physiol. Nutr. Metab.

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cides et davantage de lipides que les hommes. Les effets séparés d'une alimentation riche v en glucides ou de l'ingestion de glucose pendant l'exercice sur la sélection des substrats furent pourtant similaires chez les deux sexes. L'effet combiné des deux procédures de supplémentation est toutefois plus important chez la femme que chez l'homme, soutenant l'hypothèse qu'un léger déficit en glucides soit présent chez les femmes.Dans la troisième étude, l'oxydation des substrats et particulièrement celle d'amidon exogène au cours d'une marche prolongée à une faible puissance de travail a été décrite.Les individus qui pratiquent des activités physiques prolongées à des intensités faibles (< 40 %VO 2 max) sont encouragés à ingérer des glucides et de l'eau pendant l'effort, mais la contribution de leur oxydation à la fourniture d'énergie est relativement peu connue.Nous avons montré que, contrairement aux observations précédemment effectuées à jeun sans ingestion de glucides pendant l'effort, les glucides (incluant de source exogène) peuvent fournir une très grande partie de l'énergie lorsqu'ils sont ingérés à des intervalles réguliers au cours de l'exercice prolongé.Dans l'ensemble, les résultats des études expérimentales présentées dans cette thèse montrent que les glucides ingérés peuvent fournir une grande proportion de l'énergie pendant l'exercice prolongé. Toutefois, le mode d'exercice, le sexe et la puissance de travail mènent à des variations qui sont en grande partie liées à une dépense énergé-tique variable selon les conditions et les groupes d'individus ayant des caractéristiques différentes.Mots clés: calorimétrie indirecte respiratoire, sexe, mode d'exercice, marche. ABSTRACTThe main substrates oxidized during prolonged exercise, whether carbohydrates, fats or proteins, do not contribute equally to the energy supply. In addition, glucose can originate from different endogenous (muscle, liver) and exogenous sources. Many factors can affect their respective contribution including: active muscle mass, training, sex, nutritional state and environmental conditions. The use of stable isotopes, such as carbon 13 ( 13 C), combined with indirect respiratory calorimetry corrected for urea excretion in urine and sweat, allows the differentiation of endogenous and exogenous substrates and the estimation of their contribution to the energy yield.These methods, allowing the measurement of fuel selection during prolonged exercise with glucose ingestion, enabled the comparisons found in the three experimental studies part of this thesis. In the first study, fuel selection was compared during prolonged upper-and lower-body exercise with or without glucose ingestion. The difference in fuel selection between upper-and lower-body exercise when water was ingested was modest with a slightly higher reliance on carbohydrate oxidation during upper-body exercise. The amount of exogenous glucose oxidized was lower but its contribution to the energy yield was higher during upper-body exercise due to the lower energy expenditure.In t...

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Quantitative evaluation of the oxidation of an exogenous glucose load using naturally labeled 13C-glucose

Cited by 67 publications

References 13 publications

Lactose Assimilation by Full-Term Infants: Relation of [13C] and H2 Breath Tests with Fecal [13C] Excretion

Lactose Assimilation by Full-Term Infants: Relation of [13C] and H2 Breath Tests with Fecal [13C] Excretion

Dose-response relationship between fat ingestion and oxidation: quantitative estimation using whole-body calorimetry and 13C isotope ratio mass spectrometry

Sélection des substrats lors de l’exercice prolongé avec ingestion de glucides : études par traçage au ¹³C

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Quantitative evaluation of the oxidation of an exogenous glucose load using naturally labeled 13C-glucose

Cited by 67 publications

References 13 publications

Lactose Assimilation by Full-Term Infants: Relation of [13C] and H2 Breath Tests with Fecal [13C] Excretion

Lactose Assimilation by Full-Term Infants: Relation of [13C] and H2 Breath Tests with Fecal [13C] Excretion

Dose-response relationship between fat ingestion and oxidation: quantitative estimation using whole-body calorimetry and 13C isotope ratio mass spectrometry

Sélection des substrats lors de l’exercice prolongé avec ingestion de glucides : études par traçage au 13C

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Sélection des substrats lors de l’exercice prolongé avec ingestion de glucides : études par traçage au ¹³C