Quantitative evaluation of the immunodeficiency of a mouse strain by tumor engraftments

Ye, Wei; Jiang, Zhiwu; Li, Guan Xiong; Xiao, Yiren; Lin, Shudong; Lai, Yunxin; Wang, Suna; Li, Baiheng; Jia, Bei; Li, Yin; Huang, Zhi; Li, Jin; Feng, Fenglan; Li, Shuhua; Yao, Heng‐Chen; Liu, Zixia; Cao, Su Mei; Xu, Lin; Li, Yangqiu; Wu, Donghai; Zeng, Lingwen; Zhong, Ming; Liu, Pentao; Wang, Zhe; Xu, Bing; Yao, Yao; Pei, Duanqing; Li, Peng

doi:10.1186/s13045-015-0156-y

Cited by 46 publications

(41 citation statements)

References 35 publications

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“…However, in the Rag-2 knockout (KO) mice used by Chevez-Barrios et al (2000), the animals were intravitreally injected with Y79 cells in a similar manner as in our experiment, but the mice already developed metastases 4 weeks after the injection (Chevez-Barrios et al, 2000). These results are consistent with those of other groups, who could show that metastasization metastasesin Rag-2 KO mouse models are stronger than in nude mice for several human cancer xenografts like sarcoma (Nanni et al, 2010), breast cancer (Nanni et al, 2012) or adenocarcinoma (Ye et al, 2015). This should be considered when choosing a model.…”

Section: Discussionsupporting

confidence: 93%

Establishment of a novel retinoblastoma (Rb) nude mouse model by intravitreal injection of human Rb Y79 cells – comparison of in vivo analysis versus histological follow up

et al. 2016

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Retinoblastoma (Rb) is the most frequent primary intraocular tumour in children and, if left untreated, can cause death. Preclinical animal models that mimic molecular, genetic, and cellular features of cancers are essential for studying cancer and searching for promising diagnosis and treatment modalities. There are several models described for Rb, but none of them fully meet our requirements. The aim of this study was to create a novel xenograft-nude mouse-model with broad application possibilities, which closely resembles the clinical observations of Rb patients and which could be used to investigate the development and spread of the tumour by using scanning laser ophthalmoscopy/optical coherence tomography (SLO/OCT) as well as histology methods. We injected human retinoblastoma Y79 cells intravitreally in both eyes of immune-deficient nude mice. The incidences of retinoblastoma as well as growth velocity were analysed 3, 6, 9 and 12 weeks after cell injection in vivo by SLO/OCT as well as ex vivo by electron microscopy (EM) and hematoxylin/eosin (HE) staining. Moreover, internal organs were histologically screened for potentially occurring metastases. Three weeks post-injection, animals developed a retinoblastoma, and after five weeks tumour growth resulted in swelling of the eyes in individual animals, showing a similar phenotype to that of untreated Rb patients at advanced stages of tumour-development. After 12 weeks, 67.5% of all analysed eyes (29 of 42) contained a retinoblastoma. At early stages of Rb development, the SLO/OCT analysis correlated with the histology results. If the tumours were too large, only histological investigations were feasible. The ultrastructural characteristics of the xenograft-tumours were very similar to those described for patient's tumours. In one mouse, brain metastases were observed. Our retinoblastoma mouse model closely resembles the human disease. SLO/OCT can be used for the detection of Rb at early stages of development and could be used for monitoring the success of future therapies.

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Section: Discussionsupporting

confidence: 93%

Establishment of a novel retinoblastoma (Rb) nude mouse model by intravitreal injection of human Rb Y79 cells – comparison of in vivo analysis versus histological follow up

et al. 2016

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“…The diagnosis of HCC was confirmed by histologic analysis in all cases. HCC tissues were transplanted into NOD/SCID/IL2rg −/− (NSI) mice that were sourced from Li’s lab (15–17). Primary HCC tumors were placed in RPMI 1640 in an ice bath.…”

Section: Methodsmentioning

confidence: 99%

Anti-GPC3-CAR T Cells Suppress the Growth of Tumor Cells in Patient-Derived Xenografts of Hepatocellular Carcinoma

Jiang

Chen³

et al. 2017

Front. Immunol.

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BackgroundThe lack of a general clinic-relevant model for human cancer is a major impediment to the acceleration of novel therapeutic approaches for clinical use. We propose to establish and characterize primary human hepatocellular carcinoma (HCC) xenografts that can be used to evaluate the cytotoxicity of adoptive chimeric antigen receptor (CAR) T cells and accelerate the clinical translation of CAR T cells used in HCC.MethodsPrimary HCCs were used to establish the xenografts. The morphology, immunological markers, and gene expression characteristics of xenografts were detected and compared to those of the corresponding primary tumors. CAR T cells were adoptively transplanted into patient-derived xenograft (PDX) models of HCC. The cytotoxicity of CAR T cells in vivo was evaluated.ResultsPDX1, PDX2, and PDX3 were established using primary tumors from three individual HCC patients. All three PDXs maintained original tumor characteristics in their morphology, immunological markers, and gene expression. Tumors in PDX1 grew relatively slower than that in PDX2 and PDX3. Glypican 3 (GPC3)-CAR T cells efficiently suppressed tumor growth in PDX3 and impressively eradicated tumor cells from PDX1 and PDX2, in which GPC3 proteins were highly expressed.ConclusionGPC3-CAR T cells were capable of effectively eliminating tumors in PDX model of HCC. Therefore, GPC3-CAR T cell therapy is a promising candidate for HCC treatment.

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“…32 Here, we generated human NSCLC PDX mice by subcutaneously or intravenously implanting dissected primary tumor masses or cell suspensions that could be serially transplanted and engrafted in NSI mice. Immunohistochemistry results showed that HLA C NSCLC cells from a patient (patient P2) that had been engrafted in the lungs of NSI mice expressed E-cadherin, but not vimentin, during both the first and second passages after transplantation (Fig.…”

Section: Pdx Models Retained Molecular Phenotype Of Nsclc Cellsmentioning

confidence: 99%

PSCA and MUC1 in non-small-cell lung cancer as targets of chimeric antigen receptor T cells

et al. 2017

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Quantitative evaluation of the immunodeficiency of a mouse strain by tumor engraftments

Cited by 46 publications

References 35 publications

Establishment of a novel retinoblastoma (Rb) nude mouse model by intravitreal injection of human Rb Y79 cells – comparison of in vivo analysis versus histological follow up

Establishment of a novel retinoblastoma (Rb) nude mouse model by intravitreal injection of human Rb Y79 cells – comparison of in vivo analysis versus histological follow up

Anti-GPC3-CAR T Cells Suppress the Growth of Tumor Cells in Patient-Derived Xenografts of Hepatocellular Carcinoma

PSCA and MUC1 in non-small-cell lung cancer as targets of chimeric antigen receptor T cells

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