Quantitative evaluation of simian immunodeficiency virus infection using NASBA technology

Romano, Joseph; Shurtliff, Roxanne N.; Dobratz, Eric; Gibson, Andrew; Hickman, Kathy; Markham, Phillip D.; Pal, Ranajit

doi:10.1016/s0166-0934(99)00184-6

Cited by 79 publications

(57 citation statements)

References 20 publications

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“…The virus pellet was then lysed in 1 ml lysis buffer. Nucleic acid was isolated as described previously 48 and then analyzed by real-time nucleic acid sequence-based amplification as described before. 49 The real-time nucleic acid sequence-based amplification assay had a lower limit of sensitivity of 50 copies of RNA.…”

Section: Viral Load Measurementmentioning

confidence: 99%

Altered balance between Th17 and Th1 cells at mucosal sites predicts AIDS progression in simian immunodeficiency virus-infected macaques

et al. 2008

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Loss of CD4 + T cells in the gut is necessary but not sufficient to cause AIDS in animal models, raising the possibility that a differential loss of CD4 + T-cell subtypes may be important. We found that CD4 + T cells that produce interleukin (IL)-17, a recently identified lineage of effector CD4 + T-helper cells, are infected by SIV mac251 in vitro and in vivo, and are found at lower frequency at mucosal and systemic sites within a few weeks from infection. In highly viremic animals, Th1 cells predominates over Th17 T cells and the frequency of Th17 cells at mucosal sites is negatively correlated with plasma virus level. Because Th17 cells play a central role in innate and adaptive immune response to extracellular bacteria, our finding may explain the chronic enteropathy in human immunodeficiency virus (HIV) infection. Thus, therapeutic approaches that reconstitute an adequate balance between Th1 and Th17 may be beneficial in the treatment of HIV infection.

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Section: Viral Load Measurementmentioning

confidence: 99%

Altered balance between Th17 and Th1 cells at mucosal sites predicts AIDS progression in simian immunodeficiency virus-infected macaques

et al. 2008

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“…The virus pellet was then lysed in 1 ml of lysis buffer. Nucleic acid was isolated as described previously (32) and then analyzed by real-time NASBA as described previously (33). The real-time NASBA assay had a lower limit of sensitivity of 50 copies of RNA.…”

Section: Viral Load Measurementmentioning

confidence: 99%

Immune Activation Driven by CTLA-4 Blockade Augments Viral Replication at Mucosal Sites in Simian Immunodeficiency Virus Infection

Cecchinato

Tryniszewska

Zhong

et al. 2008

The Journal of Immunology

117

116

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The importance of chronic immune activation in progression to AIDS has been inferred by correlative studies in HIV-infected individuals and in nonhuman primate models of SIV infection. Using the SIVmac251 macaque model, we directly address the impact of immune activation by inhibiting CTLA-4, an immunoregulatory molecule expressed on activated T cells and a subset of regulatory T cells. We found that CTLA-4 blockade significantly increased T cell activation and viral replication in primary SIVmac251 infection, particularly at mucosal sites, and increased IDO expression and activity. Accordingly, protracted treatment with anti-CTLA-4 Ab of macaques chronically infected with SIVmac251 decreased responsiveness to antiretroviral therapy and abrogated the ability of therapeutic T cell vaccines to decrease viral set point. These data provide the first direct evidence that immune activation drives viral replication, and suggest caution in the use of therapeutic approaches for HIV infection in vivo that increase CD4+ T cell proliferation.

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“…Viral RNA in plasma was determined by nucleic acid sequence-based amplification (NASBA) as described [52]. Sensitivity of the NASBA assay is less than 2000 viral copies/input volume.…”

Section: Viral Rna and Proviral Dna Detectionmentioning

confidence: 99%

Comparative study of Tat vaccine regimens in Mauritian cynomolgus and Indian rhesus macaques: Influence of Mauritian MHC haplotypes on susceptibility/resistance to SHIV89.6P infection

Florese

Wiseman

Venzon

et al. 2008

Vaccine

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Protection afforded by HIV Tat-based vaccines has differed in Indian rhesus and Mauritian cynomolgus macaques. We evaluated native Tat and Ad-HIVtat priming/Tat-boosting regimens in both species. Both vaccines were immunogenic. Only the Ad-tat regimen modestly reduced acute viremia in rhesus macaques after SHIV 89.6P challenge. Confounding variables uncovered in Mauritian macaques included significant associations of susceptibility to infection with MHC class IB and class II H2 and H5 haplotypes, and resistance to infection with class IB haplotypes H3 and H6. Although protection here was limited, Tat-based vaccines incorporating other HIV components have shown greater efficacy. Combination strategies should be further explored.

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Quantitative evaluation of simian immunodeficiency virus infection using NASBA technology

Cited by 79 publications

References 20 publications

Altered balance between Th17 and Th1 cells at mucosal sites predicts AIDS progression in simian immunodeficiency virus-infected macaques

Altered balance between Th17 and Th1 cells at mucosal sites predicts AIDS progression in simian immunodeficiency virus-infected macaques

Immune Activation Driven by CTLA-4 Blockade Augments Viral Replication at Mucosal Sites in Simian Immunodeficiency Virus Infection

Comparative study of Tat vaccine regimens in Mauritian cynomolgus and Indian rhesus macaques: Influence of Mauritian MHC haplotypes on susceptibility/resistance to SHIV89.6P infection

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