“…The most common methods to determine selenium in various samples are UV-visible spectrophotometry (Arikan, Tuncay, & Apak, 1996;Johansson, Andersson, & Olin, 1995), high performance liquid chromatography (Hawkes & Kutnink, 1996;Yoshida et al, 2001), hydride generation atomic absorption spectrometry (Schloske, Waldner, & Marx, 2002;Zhang & Adeloju, 2008), atomic absorption spectrometry (Bidari, Jahromi, Assadi, & Hosseini, 2007;Saygi, Melek, Tuzen, & Soylak, 2007;, hydride generation atomic fluorescence spectrometry (Mazej, Falnoga, Veber, & Stibilj, 2006), and inductively coupled plasma mass spectrometry (Montes-Bayon, Diaz-Molet, Gonzalez, & Sanz-Medel, 2006 also requires large amounts of toxic organic solvents (Marczenko, 1986;Psillakis & Kalogerakis, 2003). Solid phase microextraction (SPME) is a solvent-free sample preparation technique and has been developed for pretreatment of various samples.…”