Quantitative evaluation of remineralizing potential of three agents on artificially demineralized human enamel using scanning electron microscopy imaging and energy-dispersive analytical X-ray element analysis: An in vitro study

Khanduri, Nitin; Kurup, Deepak; Mitra, Malay

doi:10.4103/1735-3327.294329

Cited by 27 publications

(22 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to fluoride varnishes, other materials with remineralizing potentials such as biomimetic hydroxyapatite [ 3 ] and phosphopeptide amorphous calcium phosphate [ 48 ] have exhibited promising results. Further studies evaluating the remineralization potential of these materials combined with CAP are recommended using qualitative and quantitative analyses.…”

Section: Discussionmentioning

confidence: 99%

Effect of Cold Atmospheric Pressure Plasma Coupled with Resin-Containing and Xylitol-Containing Fluoride Varnishes on Enamel Erosion

Khoubrouypak

Abbasi

Ahmadi

et al. 2021

International Journal of Dentistry

View full text Add to dashboard Cite

Purpose. Considering the suggested advantages of cold atmospheric plasma (CAP) in increasing the fluoride uptake by the enamel, this study aimed to assess enamel erosion following the application of helium CAP and two types of fluoride varnishes. Methods. The microhardness of 70 bovine enamel specimens was measured using a Vickers hardness tester. The specimens were randomly divided into 7 groups (n = 10): control, CAP (P), resin-containing fluoride varnish (RF), CAP + resin-containing fluoride varnish (PRF), fluoride varnish (F), CAP + fluoride varnish (PF), and erosion (E). The specimens in the control and erosion groups did not receive CAP or fluoride varnish. All specimens underwent erosive challenge 4 times/day using hydrochloric acid and artificial saliva except for the control specimens that remained in distilled water during the course of the study. After 5 days of erosive challenge, microhardness was measured again, and the percentage of microhardness change was calculated. Surface roughness of two specimens in each group was assessed by atomic force microscopy (AFM). Data were analyzed using one-way ANOVA followed by Tamhane’s post-hoc test. Results. The percentage of microhardness change in all groups was significantly higher than that of the control group. All groups showed significantly lower percentage of microhardness change compared with the E group except for the P group; no significant difference was noted in microhardness change of P and E groups. Other experimental groups had no significant difference with each other. Surface roughness was the highest in PRF and the lowest in the F group. Conclusion. CAP application had no significant effect on increasing the enamel resistance to erosion. However, enamel resistance to erosion increased significantly after fluoride varnish application alone or fluoride varnish application combined with CAP. No significant difference was noted between the two types of varnishes in this regard. CAP increased the surface roughness while fluoride varnish application alone decreased the roughness.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of Cold Atmospheric Pressure Plasma Coupled with Resin-Containing and Xylitol-Containing Fluoride Varnishes on Enamel Erosion

Khoubrouypak

Abbasi

Ahmadi

et al. 2021

International Journal of Dentistry

View full text Add to dashboard Cite

show abstract

“…Historically, fluoride has been the first attempt to obtain enamel remineralization [7][8][9]. Subsequently, new compounds have been recently introduced, for instance, casein phospho-peptide and amorphous calcium-phosphate [10] and biomimicry hydroxyapatite [11], all showing promising results. Although fluoride is further stable and is more acid-resistant than HA crystal, there is a restricted remineralized process due to the quantity of calcium and phosphate ions obtainable in the oral fluid [5,12].…”

Section: Introductionmentioning

confidence: 99%

Nano-Hydroxyapatite Gel and Its Effects on Remineralization of Artificial Carious Lesions

Juntavee

Sinagpulo

2021

International Journal of Dentistry

View full text Add to dashboard Cite

Introduction. Nano-hydroxyapatite gel (NHG) has never been investigated for enamel remineralization. This study evaluated the effects of two concentrations of NHG on remineralization of an artificial carious lesion in comparison with nano-HA toothpaste (NHT) and fluoride varnish (FV). Materials and Methods. Carious lesions were prepared on 100 enamel samples and divided into 5 groups: FV, NHT, 20% NHG, and 30% NHG. One untreated (NT) group was left as control. The hardness of the surface was evaluated before, during, and after remineralization. Microhardness at various phases and the percent recovery of hardness (%HR) were determined and analyzed with ANOVA. Polarized-light micrographs (PLM) were evaluated for depth of the carious lesion. Results. Significantly different remineralization capability was indicated for tested agents ( p < 0.05 ). NHT was significantly capable of remineralization greater than NHG, FV, and NT ( p < 0.05 ). No noticeable difference in %HR between 20% NHG and 30% NHG ( p > 0.05 ) was found. Decreasing in the depth of caries lesion was notified by PLM as applying either NHT or NHG as greater than FV, with no reduction in the depth for NT. Conclusions. Nano-HA both in toothpaste and gel form was capable of remineralization better than fluoride varnish. Comparable remineralization of 20% versus 30% NHG was evidenced. NHG for both concentrations was recommended as a capable remineralizing agent for caries remineralization. Clinical Significance: This study indicated that an application of nano-HA gel is an attractive route to deliver the material and can be more effective and less toxic than conventional formulations and provide its effectiveness directly at the site of action, especially for a noncooperative young child and medicinally intimidated patients who may face with inconvenience in using toothbrush and toothpaste for hygiene control.

show abstract

“…Possible preventive therapies for the treatment of MIH could be beneficial. Some remineralizing agents have been proposed for the treatment of enamel hypomineralization, in addition to conventional fluoride-based therapies, biomimetic hydroxyapatite, and casein phosphopeptide-amorphous calcium phosphate have also been recently introduced and have shown promising results [ 37 , 38 , 39 ]. Oral health promotion programs should be aimed at centers and schools for children with intellectual disabilities and should include regular use of oral health services, oral hygiene education, and dietary counseling, which is especially important when a child has MIH, a major risk factor for caries.…”

Section: Discussionmentioning

confidence: 99%

Molar Incisor Hypomineralization in Children with Intellectual Disabilities

Rajić

Modrić²,

Malčić

et al. 2021

Dentistry Journal

View full text Add to dashboard Cite

The aim of the study is to compare the frequency and the distribution of molar incisor hypomineralization (MIH) in children with intellectual disabilities. Methods: Seventy-two children with intellectual disabilities and 72 healthy children were included in the study. They ranged in age from 5 to 18 years with the same distribution by age and sex. Standard clinical examination was performed, at a dental clinic or in the institution where the children lived, by using a dental mirror and a probe, according the European Academy of Paediatric Dentistry judgment criteria for MIH. Results: Among the 72 children with intellectual disabilities, eight children (11.1%) presented MIH with 19 affected teeth. In the control group, one child (1.4%) presented MIH with two affected teeth. The difference was statistically significant (p = 0.033). There were no statistically significant differences between boys and girls. The molars, especially the first right molars were the most affected tooth. Brown defects were less common than white defects. Conclusion: Children with MIH should be identified because this condition is a common problem in children with intellectual disabilities.

show abstract

Quantitative evaluation of remineralizing potential of three agents on artificially demineralized human enamel using scanning electron microscopy imaging and energy-dispersive analytical X-ray element analysis: An in vitro study

Cited by 27 publications

References 13 publications

Effect of Cold Atmospheric Pressure Plasma Coupled with Resin-Containing and Xylitol-Containing Fluoride Varnishes on Enamel Erosion

Effect of Cold Atmospheric Pressure Plasma Coupled with Resin-Containing and Xylitol-Containing Fluoride Varnishes on Enamel Erosion

Nano-Hydroxyapatite Gel and Its Effects on Remineralization of Artificial Carious Lesions

Molar Incisor Hypomineralization in Children with Intellectual Disabilities

Contact Info

Product

Resources

About