Quantitative evaluation of normal spinal osseous metabolism with 18F-NaF PET/CT

Ayubcha, Cyrus; Zadeh, Mahdi Zirakchian; Stochkendahl, Mette Jensen; Al-Zaghal, Abdullah; Hartvigsen, Jan; Rajapakse, Chamith S.; Raynor, William; Werner, Thomas; Thomassen, Anders; Zhuang, Hongming; Høilund‐Carlsen, Poul Flemming; Alavi, Abass

doi:10.1097/mnm.0000000000000891

Cited by 19 publications

(12 citation statements)

References 36 publications

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“…In the regional image quality assessment, the skull, thoracic and lumbar spine, and proximal long bones were scored excellent and acceptable, with the thoracic and lumbar spine scoring the highest on image quality. This finding is consistent with a study in skeletally immature healthy canines ( 53 ), and a human study of the spine with [ 18 F]NaF in healthy individuals were thoracic and lumbar spine had significant higher uptake compared to the cervical spine ( 54 ). An intriguing finding of the current study was that the cervical spine images were scored excellent and acceptable for most of the samples (96%) and 4% were scored insufficient.…”

Section: Discussionsupporting

confidence: 91%

[18F] Sodium Fluoride Dose Reduction Enabled by Digital Photon Counting PET/CT for Evaluation of Osteoblastic Activity

et al. 2022

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The aim of the study was to assess the quality and reproducibility of reducing the injected [18F] sodium fluoride ([18F]NaF) dose while maintaining diagnostic imaging quality in bone imaging in a preclinical skeletal model using digital photon counting PET (dPET) detector technology. Beagles (n = 9) were administered three different [18F]NaF doses: 111 MBq (n = 5), 20 MBq (n = 5), and 1.9 MBq (n = 9). Imaging started ≃45 min post-injection for ≃30 min total acquisition time. Images were reconstructed using Time-of-Flight, ultra-high definition (voxel size of 1 × 1 × 1 mm3), with 3 iterations and 3 subsets. Point spread function was modeled and Gaussian filtering was applied. Skeleton qualitative and quantitative molecular image assessment was performed. The overall diagnostic quality of all images scored excellent (61%) and acceptable (39%) by all the reviewers. [18F]NaF SUVmean showed no statistically significant differences among the three doses in any of the region of interest assessed. This study demonstrated that a 60-fold [18F]NaF dose reduction was not significantly different from the highest dose, and it had not significant effect on overall image quality and quantitative accuracy. In the future, ultra-low dose [18F]NaF dPET/CT imaging may significantly decrease PET radiation exposure to preclinical subjects and personnel.

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Section: Discussionsupporting

confidence: 91%

[18F] Sodium Fluoride Dose Reduction Enabled by Digital Photon Counting PET/CT for Evaluation of Osteoblastic Activity

et al. 2022

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“…59 Although the thorough analysis of blood flow, metabolic rate, bone turnover, the pharmacokinetics of [ 18 F]NaF provides more information in the assessment of osteoporosis, the quantitative analysis of ROIs by SUVmean is a more simple and convenient way to obtain valuable results. 60 In the present study, RNA sequencing results showed that the expression of Col10a1 and Col2a1 genes were upregulated in the bone tissues of the mice treated with WJ-MSC-EVs, and both genes have key roles in entochondrostosis. 61 During chondrocyte maturation, Col10a1 is specifically expressed by hypertrophic chondrocytes in the hypertrophic zone of the growth plate, whereas the mutation of Col10a1 might cause growth plate defects and skeletal disorders.…”

Section: Discussionsupporting

confidence: 50%

Section: Discussionmentioning

confidence: 99%

Multiplexed Molecular Imaging Strategy Integrated with RNA Sequencing in the Assessment of the Therapeutic Effect of Wharton’s Jelly Mesenchymal Stem Cell-Derived Extracellular Vesicles for Osteoporosis

Lu¹,

Chen²,

Ke³

et al. 2021

IJN

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Introduction:Osteoporosis is a result of an imbalance in bone remodeling. Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have been considered as a potentially promising treatment for osteoporosis. However, the therapeutic effect, genetic alterations, and in vivo behavior of exogenous EVs for osteoporosis in mice models remain poorly understood. Methods: A multiplexed molecular imaging strategy was constructed by micro-positron emission tomography (µPET)/computed tomography (CT), µCT, and optical imaging modality which reflected the osteoblastic activity, microstructure, and in vivo behavior of EVs, respectively. RNA sequencing was used to analyze the cargo of EVs, and the bone tissues of ovariectomized (OVX) mice post EV treatment. Results: The result of [ 18 F]NaF µPET showed an increase in osteoblastic activity in the distal femur of EV-treated mice, and the bone structural parameters derived from µCT were also improved. In terms of in vivo behavior of exogenous EVs, fluorescent dye-labeled EVs could target the distal femur of mice, whereas the uptakes of bone tissues were not significantly different between OVX mice and healthy mice. RNA sequencing demonstrated upregulation of ECMrelated genes, which might associate with the PI3K/AKT signaling pathway, in line with the results of microRNA analysis showing that mir-21, mir-29, mir-221, and let-7a were enriched in Wharton's jelly-MSC-EVs and correlated to the BMP and PI3K/AKT signaling pathways. Conclusion:The therapeutic effect of exogenous WJ-MSC-EVs in the treatment of osteoporosis was successfully assessed by a multiplexed molecular imaging strategy. The RNA sequencing demonstrated the possible molecular targets in the regulation of bone remodeling. The results highlight the novelty of diagnostic and therapeutic strategies of EV-based treatment for osteoporosis.

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“…[80][81][82] The observation that NaF uptake at the cervical, thoracic, and lumbar spine is associated with increased body weight in healthy individuals suggests that NaF-PET/CT can detect early changes in the spine related to degeneration. 83 In 42 adult patients with back pain and no history of spine surgery, NaF-PET/CT scans identified the cause of pain in 37 (88%) of the patients. 82 Furthermore, NaF activity at lumbar facet joints has been found to correlate with both MR findings and clinical disability.…”

Section: Back Painmentioning

confidence: 99%

Novel Musculoskeletal and Orthopedic Applications of 18F-Sodium Fluoride PET

et al. 2021

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The bone-seeking properties of 18 F-sodium fluoride (NaF) were first described in 1962 by Blau and colleagues, 1 who used this positron-emitting radiotracer in skeletal scintigraphy. Both high uptake by bone and rapid plasma clearance of NaF were determined to be advantages in imaging skeletal metabolism. 2 The mechanism of NaF uptake relies on the exchange of OH À for 18 F À , converting hydroxyapatite on the surface of the bone matrix to fluorapatite. 2,3 One hour after NaF is administered intravenously, only 10% remains in the plasma compartment because of a first-pass Conflict of interest: The authors have declared no conflicts of interest.

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Quantitative evaluation of normal spinal osseous metabolism with 18F-NaF PET/CT

Cited by 19 publications

References 36 publications

[18F] Sodium Fluoride Dose Reduction Enabled by Digital Photon Counting PET/CT for Evaluation of Osteoblastic Activity

[18F] Sodium Fluoride Dose Reduction Enabled by Digital Photon Counting PET/CT for Evaluation of Osteoblastic Activity

Multiplexed Molecular Imaging Strategy Integrated with RNA Sequencing in the Assessment of the Therapeutic Effect of Wharton’s Jelly Mesenchymal Stem Cell-Derived Extracellular Vesicles for Osteoporosis

Novel Musculoskeletal and Orthopedic Applications of 18F-Sodium Fluoride PET

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