Quantitative evaluation of macrophage expression using CD68 in oral submucous fibrosis: An immunohistochemical study

Pereira, Treville; Naik, Shantala R; Tamgadge, Avinash

doi:10.4103/2141-9248.177983

Cited by 11 publications

(4 citation statements)

References 19 publications

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“…Macrophages are known to drive fibrosis by producing profibrotic mediators including TGF-β1 and platelet-derived growth factor (PDGF) that directly activate fibroblasts and control extracellular matrix turnover by regulating the balance of MMPs and TIMPs 16 . The expression of CD68 was increased in rat models of liver fibrosis 18 and in patients with intermediate and advanced stages of oral submucous fibrosis 19 and idiopathic pulmonary fibrosis (IPF) 20 . The infiltration of macrophages (CD68 + cells) in category I and IIA endometria using immunofluorescence staining was confirmed.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic profiling of mare endometrium at different stages of endometrosis

Szóstek-Mioduchowska,

Wójtowicz,

Sadowska

et al. 2023

Sci Rep

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In the current study, transcriptome profiles of mare endometrium, classified into categories I, IIA, and IIB according to Kenney and Doig, were compared using RNA sequencing, analyzed, and functionally annotated using in silico analysis. In the mild stage (IIA) of endometrosis compared to category I endometrium, differentially expressed genes (DEGs) were annotated to inflammation, abnormal metabolism, wound healing, and quantity of connective tissue. In the moderate stage (IIB) of endometrosis compared to category I endometrium, DEGs were annotated to inflammation, fibrosis, cellular homeostasis, mitochondrial dysfunction, and pregnancy disorders. Ingenuity pathway analysis (IPA) identified cytokines such as transforming growth factor (TGF)-β1, interleukin (IL)-4, IL-13, and IL-17 as upstream regulators of DEGs associated with cellular homeostasis, metabolism, and fibrosis signaling pathways. In vitro studies showed the effect of these cytokines on DEGs such as ADAMTS1, -4, -5, -9, and HK2 in endometrial fibroblasts at different stages of endometrosis. The effect of cytokines on ADAMTS members’ gene transcription in fibroblasts differs according to the severity of endometrosis. The identified transcriptomic changes associated with endometrosis suggest that inflammation and metabolic changes are features of mild and moderate stages of endometrosis. The changes of ADAMTS-1, -4, -5, -9, in fibrotic endometrium as well as in endometrial fibroblast in response to TGF-β1, IL-4, IL-13, and IL-17 suggest the important role of these factors in the development of endometrosis.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic profiling of mare endometrium at different stages of endometrosis

Szóstek-Mioduchowska,

Wójtowicz,

Sadowska

et al. 2023

Sci Rep

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“…The immunostaining was evaluated semiquantitatively using an intensity score (IS) (Pereira, Naik, & Tamgadge, ). In this method, the intensity score (IS) was evaluated in the following manner: −, negative; +, weak staining; ++, moderate staining; and +++, strong staining.…”

Section: Methodsmentioning

confidence: 99%

Distribution of CD68‐, CD8‐, MHCI‐ and MHCII‐positive cells in the bull and ram testis and epididymis

Saruhan

Sağsöz

Akbalık

et al. 2018

Anat Histol Embryol

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The mammalian testis possesses a special immunological environment because of its properties of remarkable immune privilege and effective local innate immunity. The testicular immune privilege protects immunogenic germ cells from systemic immune attack, and local innate immunity is important in preventing testicular microbial infections. Thus, this study aimed to immunohistochemically demonstrate the distribution and localization of CD68-, CD8-, MHCI- and MHCII-positive immune cells in the testes and epididymes. Negative immunoreactivity was detected in the seminiferous tubule epithelium and peritubular myoid cells of the testes upon staining in CD68, CD8 and MHC Class I. Positive CD68 immunoreaction was determined in the Sertoli cells and some Leydig cells. The detection of positive cells for CD8 clearly indicated the presence of lymphocytes. Furthermore, the staining with MHCI intensity was ascertained to vary from weak to moderate in the Sertoli and Leydig cells and connective tissue cells. MHCII-positive immunoreactivity was determined in myoid cells and Leydig cells in the interstitial area. The epithelium of the epididymis showed positive staining for CD68 and CD8, but the stroma displayed a rather weak staining. In the ram epididymis, neither intraepithelial nor interstitial positive reaction was observed for MHCI. In the epididymis, the basal cells displayed a stronger staining for MHCII. In conclusion, these cells not only contribute to local immunity through their direct effects on the quality of fertility in males, but also contribute either directly or indirectly to immune privilege by minimizing the development of both autoimmune reactions and potentially harmful risks.

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“…3. Immunohistochemical study: For detection of macrophages using CD68 polyclonal antibody [11] and apoptotic cells using caspase-3 polyclonal antibody [12] .…”

Section: Light Microscopymentioning

confidence: 99%

The Possible Protective Role of Echinacea and Ginger and both of them on the Lung of Diabetic Male Rats: Histological and Immunohistochemical Study

Ahmed

Abdel-Aziz

Elsayed

et al. 2021

Egyptian Journal of Histology

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Introduction:Diabetes is a systemic disease characterized by a state of hyperglycemia. Several changes in the respiratory functions had been detected in diabetic patients. Aim of the Work:The current study estimated the efficiency of two herbal extract (ginger and echinacea) on the changes in the lung of diabetic animal model. Materials and Methods: 50 adult male albino rats were used. They were divided into five groups. Control group (group I) received regular diet. Group II: diabetic group (diabetes induced by a single intraperitoneal injection of streptozotocin 60 mg/ kg). Group III: diabetic rats treated with 100mg/kg echinacea orally for 30 days. Group IV: diabetic rats treated with 400mg/kg ginger orally for 30 days. Group V: diabetic rats treated with both 100mg/kg echinacea and 400mg/kg ginger orally for 30 days. Paraffin blocks were prepared for histological and immunohistochemical examinations. Morphometric study and statistical analysis were done for the thickness of the interalveolar septum, the area percentage of collagen fibers and number of positive caspase -3 cells and CD68 positive macrophages. Ultrathin sections were prepared for electron microscopic examination. Results: Diabetes caused a significant increase in the thickness of the interalveolar septa, the area percentage of collagen fibers, the number of caspase-3 positive cells as well as the number of CD68 positive alveolar macrophages compared to the control group. Ultrastructurally, pneumocyte type II appeared with denser nucleus, numerous vacuoles and disorganized lamellar bodies. Conclusion: Groups treated with echinacea or ginger or both showed a significant decrease in the thickness of the interalveolar septa, the area percentage of collagen fibers, the number of caspase-3 positive cells as well as the number of CD68 positive alveolar macrophages compared to the diabetic group. These groups also showed that pneumocyte type II restored their normal appearance.

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Quantitative evaluation of macrophage expression using CD68 in oral submucous fibrosis: An immunohistochemical study

Cited by 11 publications

References 19 publications

Transcriptomic profiling of mare endometrium at different stages of endometrosis

Transcriptomic profiling of mare endometrium at different stages of endometrosis

Distribution of CD68‐, CD8‐, MHCI‐ and MHCII‐positive cells in the bull and ram testis and epididymis

The Possible Protective Role of Echinacea and Ginger and both of them on the Lung of Diabetic Male Rats: Histological and Immunohistochemical Study

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