Quantitative evaluation of collagen and elastic fibers after intense pulsed light treatment of mouse skin

Faucz, Luciana Lisboa; Will, Sonia Elisabete Alves de Lima; Rodrigues, Consuelo Junqueira; Hesse, Henrique; Moraes, Angelina Cirelli de; Maria, Durvanei Augusto

doi:10.1002/lsm.22782

Cited by 15 publications

(16 citation statements)

References 29 publications

(37 reference statements)

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“…Paraffin-embedded sections were examined using hematoxylin and eosin (H&E), Masson’s trichrome and picrosirius red staining as previously described 51,54,55 . The severity of collagen deposition and lung fibrosis was assessed by measuring the Masson’s trichrome and picrosirius red staining, respectively 56,57 .…”

Section: Methodsmentioning

confidence: 99%

Kefir peptides alleviate particulate matter <4 μm (PM4.0)-induced pulmonary inflammation by inhibiting the NF-κB pathway using luciferase transgenic mice

Chen

Hung

Yen

et al. 2019

Sci Rep

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Kefir peptides, generated by kefir grain fermentation of milk proteins, showed positive antioxidant effects, lowered blood pressure and modulated the immune response. In this study, kefir peptide was evaluated regarding their anti-inflammatory effects on particulate matter <4 μm (PM 4.0 )-induced lung inflammation in NF-κB-luciferase +/+ transgenic mice. The lungs of mice under 20 mg/kg or 10 mg/kg PM 4.0 treatments, both increased significantly the generation of reactive oxygen species (ROS) and inflammatory cytokines; increased the protein expression levels of p-NF-κB, NLRP3, caspase-1, IL-1β, TNF-α, IL-6, IL-4 and α-SMA. Thus, we choose the 10 mg/kg of PM 4.0 for animal trials; the mice were assigned to four treatment groups, including control group (saline treatment), PM 4.0 + Mock group (only PM 4.0 administration), PM 4.0 + KL group (PM 4.0 + 150 mg/kg low-dose kefir peptide) and PM 4.0 + KH group (PM 4.0 + 500 mg/kg high-dose kefir peptide). Data showed that treatment with both doses of kefir peptides decreased the PM 4.0 -induced inflammatory cell infiltration and the expression of the inflammatory mediators IL-lβ, IL-4 and TNF-α in lung tissue by inactivating NF-κB signaling. The oral administrations of kefir peptides decrease the PM 4.0 -induced lung inflammation process through the inhibition of NF-κB pathway in transgenic luciferase mice, proposing a new clinical application to particulate matter air pollution-induced pulmonary inflammation.

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Section: Methodsmentioning

confidence: 99%

Kefir peptides alleviate particulate matter <4 μm (PM4.0)-induced pulmonary inflammation by inhibiting the NF-κB pathway using luciferase transgenic mice

Chen

Hung

Yen

et al. 2019

Sci Rep

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“…13,14 Pathology specimens obtained after IPL treatment show enhanced collagen and elastic fibre formation in the papillary and sub-papillary dermis, resulting in an increase in volume which in turn leads to smoothing the upper dermal layers and the increased epidermal turnover. 15,16 In an in vitro study, Wong et al 17 showed that after IPL irradiation, the number of live fibroblasts increases more than 100%. They postulated that the IPL rejuvenation effect is concurrent with an increase in extracellular matrix volume resulting from the enhanced expression of collagen III and TGF-B1 genes.…”

Section: Discussionmentioning

confidence: 99%

The Efficacy of IPL in Periorbital Skin Rejuvenation: An Open-Label Study

et al. 2019

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Introduction: Intense pulsed light (IPL) is one of the effective methods used to treat the signs of facial aging. However, its efficacy in improving the appearance of periorbital wrinkles has always been evaluated in the context of whole-face rejuvenation and not studied in its own right. Therefore, in this study, we sought to examine the effectiveness and side effects of IPL in periorbital skin rejuvenation. Methods: In this uncontrolled, open-label clinical trial, 38 patients referred to the Behrooz professional skin clinic for signs of periorbital skin aging were recruited. The patients underwent three treatment sessions with IPL at one-month intervals and were evaluated for treatment side effects after each session. Six months after the final IPL session, patients were examined to assess the level of symptom improvement. The patients were photographed at each treatment session and on the 6-month follow-up visit. Before and after treatment, the patient images were evaluated by two dermatologists unaffiliated with the study to determine the extent of improvement in the appearance of wrinkles and skin texture. Results: Six months’ post-treatment, excellent improvement was observed in 3 patients (9.1%), considerable improvement in 7 patients (21.2%), moderate improvement in 9 patients (27.3%), mild improvement in 9 patients (27.3%), and finally little or no improvement in 5 patients (15.1%). A comparison of improvement scores based on the Fitzpatrick skin phenotype did not show significant differences (P=0.674). Four patients (12.1%) were dissatisfied with the treatment, whilst 17 patients (51.5%) reported moderate to considerable satisfaction with IPL rejuvenation. Conclusion: During facial rejuvenation, IPL can be used to improve periorbital skin aging.

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“…The photothermal effects lead to reversible thermal damage to surrounding collagen and induction contraction of collagen fibers and fiber remodeling. 50 Clinical studies on the effectiveness on skin tightening have shown incongruent results. Multiple studies have noted improvement in skin elasticity as well as increased deposits of collagen in treated areas.…”

Section: Ipl Therapymentioning

confidence: 99%

Skin Tightening

Kwan¹,

Kolansky²,

Abittan³

et al. 2020

Cutis

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There are many undesirable manifestations that arise as one ages. One of the most notable undesired effects is increasing skin laxity. Traditionally, the gold standard of treatment is surgical correction of skin laxity. However, demand for noninvasive aesthetic procedures has grown due to minimal risk and accelerated recovery time. Over the years, many new devices have become available for addressing skin laxity including laser therapy, radiofrequency (RF), ultrasound, and intense pulsed light (IPL). This article discusses these various noninvasive treatment options and seeks to give providers details of the science behind each device. Cutis. 2020;106:134-137, 139. M inimally and noninvasive skin tightening has become one of the most requested cosmetic procedures. Skin laxity often is apparent in areas of the face, neck, jawline, hands, abdomen, and thighs, with features of fine lines, wrinkles, and cellulite. Intrinsic and extrinsic factors contribute to the development of skin laxity. Intrinsic aspects include chronological age, stress, and genetics, whereas extrinsic influences include exposure to solar radiation, environmental toxins, and smoking. 1,2 These factors affect the production and maintenance of both collagen and elastic proteins, which are the main

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Quantitative evaluation of collagen and elastic fibers after intense pulsed light treatment of mouse skin

Abstract: IPL treatment significantly increased the number of collagen and elastic fibers within the dermis and improved the parallel distribution of collagen fibers in relation to the epidermis. These results were evident after three IPL treatments. Lasers Surg. Med. © 2018 Wiley Periodicals, Inc.

Cited by 15 publications

References 29 publications

Kefir peptides alleviate particulate matter <4 μm (PM4.0)-induced pulmonary inflammation by inhibiting the NF-κB pathway using luciferase transgenic mice

Kefir peptides alleviate particulate matter <4 μm (PM4.0)-induced pulmonary inflammation by inhibiting the NF-κB pathway using luciferase transgenic mice

The Efficacy of IPL in Periorbital Skin Rejuvenation: An Open-Label Study

Skin Tightening

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