Quantitative evaluation by computer-based video densitometry of film response to different isotopes and fluorographic treatments

Rd, Rulli

doi:10.1016/0003-2697(88)90372-7

Cited by 4 publications

(1 citation statement)

References 11 publications

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“…We concluded that in our immunoblotting experiments using radiolabeled antibodies we have a linear response over at least a 1 0-fold range. Therefore, we designed all subsequent experiments to be at that range and used multiple exposures of autoradiograms for quantitative analysis (Rulli, 1988). It is interesting to note that because of the differences in radiation energy, the use of 3SS-radiolabeled antibodies resulted in sharper bands than with lrs1 label.…”

Section: Resultsmentioning

confidence: 99%

Developmental Regulation of Microtubule‐Associated Protein 2 Expression in Regions of Mouse Brain

Crandall

Fischer

1989

Journal of Neurochemistry

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The relative levels of microtubule-associated protein 2(MAP2) were determined during postnatal development of the mouse in six different discrete brain regions: cerebellum, cortex, hippocampus, olfactory bulb, brainstem, and hypothalamus. Brain homogenates were electrophoresed on sodium dodecyl sulfate-containing gels and analyzed by immunoblotting with MAP2-specific antibodies. The levels of MAP2 in each region were determined using radiolabeled secondary antibodies and densitometric quantification of the autoradiograms over a range that was determined to have a linear response. The results indicated that in all regions and at all ages there was only one high-molecular-weight polypeptide of MAP2, which did not change in electrophoretic mobility after dephosphorylation. In most regions, the levels of MAP2 increased during the first 2 postnatal weeks. However, there were differences in the time course and relative levels of MAP2 between regions. In addition, all regions of the brain expressed the low-molecular-weight form of MAP2 (MAP2c) that was present at birth as a heterogeneous group of polypeptides with an apparent molecular weight of 70K. Most of the heterogeneity of MAP2c, however, was eliminated after dephosphorylation. The levels of MAP2c decreased dramatically after 2 weeks postnatally, except for the olfactory bulb, where the levels of MAP2c remained relatively high even in adults.

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Section: Resultsmentioning

confidence: 99%