Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies

Laurell, C.‐B.

doi:10.1016/0003-2697(66)90246-6

Cited by 4,370 publications

(1,118 citation statements)

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“…Plasma lipoprotein fractions (VLDL, LDL and HDL) were isolated using previously described procedures [36]. Plasma apolipoprotein B (apoB) concentrations were measured by the rocket immuno-electrophoretic method of Laurell [37].…”

Section: Methodsmentioning

confidence: 99%

High normal 2-hour plasma glucose is associated with insulin sensitivity and secretion that may predispose to type 2 diabetes

et al. 2005

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Section: Methodsmentioning

confidence: 99%

High normal 2-hour plasma glucose is associated with insulin sensitivity and secretion that may predispose to type 2 diabetes

et al. 2005

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“…Apolipoprotein (Apo) B and A-I concentrations were measured in plasma by the rocket immunoelectrophoretic method. 19 The lyophilized serum standards for apo measurements were prepared in our laboratory and calibrated with reference standards obtained from the Centers for Disease Control.…”

Section: Plasma Lipid-lipoprotein Measurementsmentioning

confidence: 99%

Hypertrophy and hyperplasia of abdominal adipose tissues in women

et al. 2007

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Objective: To examine the expression of selected transcription factors involved in adipogenesis and genes related to lipid metabolism in abdominal subcutaneous and omental fat tissue. Research design and methods: We obtained subcutaneous and omental adipose tissue samples from 40 women undergoing abdominal hysterectomies (age: 4775 years; BMI 27.975.3 kg/m 2 ). We measured isolated adipocyte size and metabolism, and detailed measures of body fat accumulation and body fat distribution were obtained (dual-energy X-ray absorptiometry and computed tomography, respectively). Results: Adipocyte size of both subcutaneous and omental fat were increased with higher body fat mass values, with similar regression slopes in each compartment. In contrast, with higher body fat mass values, fat accumulation was progressively higher in the subcutaneous than in the visceral fat compartment, suggesting hyperplasia in the subcutaneous fat compartment. Messenger RNA levels of CEBPa, PPARg2, SREBP1c and genes related to lipid metabolism (LPL, FABP4, DGAT1, DGAT2, PLIN and HSL) were significantly higher in subcutaneous than in omental fat tissue (Pp0.001 for all). Only subcutaneous expression of these genes tracked with obesity levels as reflected by significant positive associations between subcutaneous fat CEBPa, SREBP1c and DGAT2 expression and total body fat mass (r ¼ 0.37, r ¼ 0.41, r ¼ 0.57, respectively, Pp0,05), fat percentage (r ¼ 0.40, r ¼ 0.39, r ¼ 058, respectively, Pp0,05) and subcutaneous adipose tissue area (r ¼ 0.36, r ¼ 0.38, r ¼ 0.58, respectively, Pp0,05). Omental adipose tissue expression levels of these genes were not significantly related to adiposity measures. Conclusions: These results show that in obese women, hyperplasia is predominant in the subcutaneous fat depot, whereas fat cell hypertrophy is observed both in the omental and subcutaneous compartments.

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“…Methods are also available for quantifying fibrinogen antigen, often using immunological electrophoresis (e.g. Laurell rocket) or enzyme-linked immunosorbent assays (ELISA) [22]. Table II summarizes the clinical and laboratory findings for a variety of disorders affecting fibrinogen or its function.…”

Section: Coagulation Tests Used To Assess Fibrinogenmentioning

confidence: 99%

Laboratory testing for fibrinogen abnormalities

2008

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Fibrinogen is essential for the formation of a fibrin clot. Acquired and congenital disorders of fibrinogen may result in decreased concentration or altered function of fibrinogen, often leading to an increased risk of bleeding. Routine coagulation testing and specialized laboratory investigations can guide diagnosis in patients suspected of having a fibrinogen abnormality. This article summarizes the types of laboratory assays that are used to assess fibrinogen disorders, and key abnormalities found in different types of fibrinogen disorders. Am. J. Hematol. 83:928-931, 2008. V

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Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies

Cited by 4,370 publications

References 4 publications

High normal 2-hour plasma glucose is associated with insulin sensitivity and secretion that may predispose to type 2 diabetes

High normal 2-hour plasma glucose is associated with insulin sensitivity and secretion that may predispose to type 2 diabetes

Hypertrophy and hyperplasia of abdominal adipose tissues in women

Laboratory testing for fibrinogen abnormalities

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