Quantitative Estimation of p-Hydroxyphenylpyruvic Acid in Human Urine

The Role of the Adrenals in the Rise of Liver Enzymes in Tumor-bearing Rats

“…Increased excretion of p-hydroxyphenylpyruvate, the product of tyrosine trans amination, occurs in rats and guinea pigs when the activity in liver of the transaminase is elevated relative to that of the oxidase which degrades this metabolite [16]. In human connective tissue diseases, the reported [23] excretion of an abnormal metabolite of tyrosine has been disproved [24], but the excretion of increased amounts of the normal metabolite, p-hydroxyphenylpyruvate, does occur [12]. Thus, an example of a non-specific effect of disease is the increased excretion in rheumatoid arthritis of metabolites of both tryptophan [22] and tyrosine [12].…”

“…In human connective tissue diseases, the reported [23] excretion of an abnormal metabolite of tyrosine has been disproved [24], but the excretion of increased amounts of the normal metabolite, p-hydroxyphenylpyruvate, does occur [12]. Thus, an example of a non-specific effect of disease is the increased excretion in rheumatoid arthritis of metabolites of both tryptophan [22] and tyrosine [12]. The present studies, showing an increase of both trypto phan pyrrolase and tyrosine transaminase secondary to adrenocortical hyperactivity in tumor-bearing rats, suggest that increased excretions of tyrosine and tryptophan metabolites may also occur here.…”

Ultraviolet spectrometry

“…Ashworth and Keller (30) determined tertiary mercaptans by converting them to thionitrites. Holcomb and coworkers (289) determined p-hvdroxyphenylpyruvic acid in urine as p-hydroxybenzaldehyde. Scoggins and Miller (546) determined tertiary alcohols as the corresponding tertiary alkyl iodides.…”