Quantitative Estimation of Coproporphyrin III and Protoporphyrin IX from Their Mixture by Spectrofluorometry

Tewari, Arun K.; Tripathy, Baishnab C.

doi:10.1007/bf03263032

Cited by 1 publication

(1 citation statement)

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“…intermedia , which has similar fluorescent properties [ 3 , 23 ]. The interference between the fluorescence emissions of protoporphyrin IX at 632 nm and coproporphyrin III at 622 nm has already been reported [ 31 ]. Likewise, a study of the fluorescence of dental calculus was also able to resolve two excitation peaks, at 398 and 405 nm, and attributed these to the presence of ‘at least two porphyrin derivatives’ [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

A Preliminary Study of the Effects of pH upon Fluorescence in Suspensions of Prevotella intermedia

et al. 2016

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The quantification of fluorescence in dental plaque is currently being developed as a diagnostic tool to help inform and improve oral health. The oral anaerobe Prevotella intermedia exhibits red fluorescence due to the accumulation of porphyrins. pH affects the fluorescence of abiotic preparations of porphyrins caused by changes in speciation between monomers, higher aggregates and dimers, but this phenomenon has not been demonstrated in bacteria. Fluorescence spectra were obtained from suspensions of P. intermedia that were adjusted to pHs commensurate with the range found within dental plaque. Two fluorescent motifs were identified; 410 nm excitation / 634 nm emission (peak A) and 398 nm excitation / 622 nm emission (peak B). A transition in the fluorescence spectra was observed from peak A to peak B with increasing pH which was also evident as culture age increased from 24 hours to 96 hours. In addition to these ‘blue-shifts’, the intensity of peak A increased with pH whilst decreasing with culture age from 24 to 96 hours. A bacterium’s relationship with the local physiochemical environment at the time of image capture may therefore affect the quantification of dental plaque fluorescence.

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