Quantitative dried blood spot analysis for metallodrugs by laser ablation-inductively coupled plasma-mass spectrometry

Kröger, Sabrina; Sperling, Michael; Kärst, Uwe

doi:10.1016/j.jtemb.2018.09.009

Cited by 17 publications

(9 citation statements)

References 37 publications

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“…In this work, in-house solid matrix-matched standards were prepared from uranium-spiked kidney homogenate. Nevertheless, further calibration strategies are found in the literature including the solution-based calibration [27], fabrication xerogel solid calibration standards which are more suitable for the calibration of glasses and silicate matrices [28], the synthesis of metal-spiked polymer films [29], the use of dried droplets or dried matrix spots (DMS) deposited on different substrates [30][31][32][33][34], and the spiking and sectioning of gelatine droplets, whose use has increased noticeably in recent years for the quantitative analysis of biological tissues [35][36][37][38][39][40][41]. For internal standardization, three different approaches are mainly described in the literature for the quantitative elemental imaging of biological samples: (i) the simultaneous nebulization of a standard solution [42], (ii) the use of an element naturally occurring in the sample [43][44][45], and (iii) the addition of an internal standard-spiked layer between the sample and support or on the sample [46][47][48][49][50][51].…”

Section: Introductionmentioning

confidence: 99%

A novel calibration strategy based on internal standard–spiked gelatine for quantitative bio-imaging by LA-ICP-MS: application to renal localization and quantification of uranium

et al. 2020

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Mass spectrometry imaging (MSI) using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been employed for the elemental bio-distribution and quantification of uranium (U) in histological tissue sections of rodent kidneys. Kidneys were immediately immersed into 4% paraformaldehyde (PFA) solution for 24 h, Tissue-Tek O.C.T. Compound embedded and stored at − 80°C until cutting in a cryostat, and mounted in gel-covered glass slides. In order to assure complete ablation of sample, sample preparation and laser conditions were carefully optimized. In this work, a new analytical methodology is presented for performing quantitative laser ablation analyses based on internal standard (thulium, Tm)-spiked gelatine (10% m/v) for correction of matrix effects, lack of tissue homogeneity, and instrumental drift. In parallel, matrix-matched laboratory standards, dosed at different concentrations of U, were prepared from a pool of rat kidneys. The quantitative images of cryosections revealed heterogeneous distribution of uranium within the renal tissue, because the cortical concentration was up to 120fold higher than the medullary concentration.

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Section: Introductionmentioning

confidence: 99%

A novel calibration strategy based on internal standard–spiked gelatine for quantitative bio-imaging by LA-ICP-MS: application to renal localization and quantification of uranium

et al. 2020

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“…Other approaches for the study of Gd deposition were developed by Löhofer et al [26], which combined MRI and LA-ICP-MS for the detection and quantification of Gadofluorine P deposition in scar formation and myocardial remodeling in the heart after myocardial infarction. Kröger et al [23] employed a dried blood spot method to quantify the metallodrugs gadopentetate and gadoterate.…”

Section: Gadolinium Distributionmentioning

confidence: 99%

Recent advances in LA-ICP-MS for biomedical applications

Barbosa

Sussulini

2020

BSI

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The applications of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) in biomedical research are progressively increasing in the last decade. The main advantage of this powerful analytical technique is the ability to perform multi-elemental analysis directly from biological samples, such as tissues, organs, and single cells, with minimal sample preparation. This feature is particularly important for studies regarding disease biomarkers and mechanism of action of drugs. In this mini-review, the recent advances in the use of LA-ICP-MS for the analysis of biological samples will be discussed, as well as representative biomedical applications.

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“…Other laboratories used LA-ICP-MSI or related techniques to analyze the composition of human kidney stones [54], identify inflammatory hotspots associated with elevated metal quantities in placental tissue from low-birth-weight pregnancy [55], map trace and bulk elements in tumor tissue obtained from an individual previously treated with cisplatin [56], and define contaminations in human hair or teeth resulting from occupational exposure or unfavorable dental fillings [57][58][59]. Likewise, the first studies analyzed element distribution in human eye lenses, brain tissue, or skin biopsies from patients suffering from renal insufficiency [60][61][62], optimized radiodosages in treatment of tumors [63], or determined individual metals or metal-containing contrast agents in human blood or serum [64][65][66][67][68]. In addition, there are many inborn errors of metal and metalloid metabolism human trace elements disorders, in which LA-ICP-MSI offers advantages.…”

Section: Translational Aspects Of La-icp-msimentioning

confidence: 99%

Laser Ablation Inductively Coupled Plasma Spectrometry: Metal Imaging in Experimental and Clinical Wilson Disease

2019

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Wilson disease is an inherited disorder caused by mutations in the ATP7B gene resulting in copper metabolism disturbances. As a consequence, copper accumulates in different organs with most common presentation in liver and brain. Chelating agents that nonspecifically chelate copper, and promote its urinary excretion, or zinc salts interfering with the absorption of copper from the gastrointestinal tract, are current medications. Also gene therapy, restoring ATP7B gene function or trials with bis-choline tetrathiomolybdate (WTX101) removing excess copper from intracellular hepatic copper stores and increasing biliary copper excretion, is promising in reducing body’s copper content. Therapy efficacy is mostly evaluated by testing for evidence of liver disease and neurological symptoms, hepatic synthetic functions, indices of copper metabolisms, urinary copper excretions, or direct copper measurements. However, several studies conducted in patients or Wilson disease models have shown that not only the absolute concentration of copper, but also its spatial distribution within the diseased tissue is relevant for disease severity and outcome. Here we discuss laser ablation inductively coupled plasma spectrometry imaging as a novel method for accurate determination of trace element concentrations with high diagnostic sensitivity, spatial resolution, specificity, and quantification ability in experimental and clinical Wilson disease specimens.

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Quantitative dried blood spot analysis for metallodrugs by laser ablation-inductively coupled plasma-mass spectrometry

Cited by 17 publications

References 37 publications

A novel calibration strategy based on internal standard–spiked gelatine for quantitative bio-imaging by LA-ICP-MS: application to renal localization and quantification of uranium

A novel calibration strategy based on internal standard–spiked gelatine for quantitative bio-imaging by LA-ICP-MS: application to renal localization and quantification of uranium

Recent advances in LA-ICP-MS for biomedical applications

Laser Ablation Inductively Coupled Plasma Spectrometry: Metal Imaging in Experimental and Clinical Wilson Disease

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