Quantitative distribution of Salmonella spp. and Escherichia coli on beef carcasses and raw beef at retail establishments

Martínez-Chávez, Liliana; Cabrera-Díaz, E.; Pérez-Montaño, Julia A.; Garay-Martínez, L. E.; Varela-Hernández, Juan José; Castillo, Alejandro; Lucia, L. M.; Ávila-Novoa, María Guadalupe; Cardona-López, Marco Antonio; Gutiérrez-González, Porfirio; Martínez-Gonzáles, Nanci Edid

doi:10.1016/j.ijfoodmicro.2015.06.016

Cited by 34 publications

(27 citation statements)

References 16 publications

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“…However, in countries like Turkey and Senegal, the pathogen was isolated from 21.3 and 87.4% of ground beef samples analyzed ( Arslan & Eyi, 2010 ; Stevens et al, 2006 ). Similarly, high frequencies of Salmonella in raw meats has been reported in Mexico; the pathogen was isolated from 6.4 to 28.6% of beef carcass samples collected at small non-federally inspected abattoirs ( Perez-Montano et al, 2012 ), from 29.9% of raw beef samples collected at retail, and from 56.7 to 71% of ground beef samples obtained from butcher’s shops ( Cabrera-Diaz et al, 2013 ; Martinez-Chavez et al, 2015 ).…”

Section: Introductionmentioning

confidence: 77%

Genetic diversity and antimicrobial resistance ofSalmonellaserotypes recovered throughout the beef production chain and from patients with salmonellosis

Realpe

Barba-León

Pérez-Montaño

et al. 2018

PeerJ

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Salmonella is one of the major foodborne pathogens worldwide. The antimicrobial resistance (AMR) of this foodborne pathogen has raised a great concern in recent years. Studies on the frequency and characterization of Salmonella serotypes can help to improve our knowledge on the epidemiology of this pathogen. The purpose of this study was to compare the serotypes, AMR and genetic profiles of Salmonella isolates recovered from raw beef throughout the beef production chain and from human feces associated with clinical cases of salmonellosis. The serotype, AMR and pulsed-field gel electrophoresis profile of 243 Salmonella enterica isolates recovered from beef carcasses (n = 78), ground beef (n = 135), and human feces from clinical cases of salmonellosis (n = 30) were compared. Forty-three different Salmonella serotypes were identified and regardless of the source, the top five corresponded to Typhimurium, Give, Group B (partially serotyped), Infantis and Anatum. Twelve serotypes from beef carcasses were also found in ground beef, showing their presence throughout the beef production chain. Salmonella Typhimurium, Infantis, Anatum and Montevideo were the only serotypes identified in all sample types. Resistance to tetracyclines was the most frequent (41.2%) followed by resistance to aminoglycosides (37%), folate pathway inhibitors (21%), quinolones (20.2%), phenicols (17.1%), penicillins (15.6%) and cephems (7%). Multidrug resistance was observed in 28.8% of the isolates, and those from human feces showed resistance to a larger number of antimicrobials. Great concern arises from the resistance and reduced susceptibility observed to quinolones and cephalosporins because these drugs are the first line of treatment for invasive Salmonella infections. Twenty-seven distinct pulse-types were detected among 238 isolates. Clustering analysis for the most frequent serotypes identified groups of isolates with similar AMR profiles. Multidrug resistance spreading throughout the food production chain should be continually monitored and its importance emphasized.

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Section: Introductionmentioning

confidence: 77%

Genetic diversity and antimicrobial resistance ofSalmonellaserotypes recovered throughout the beef production chain and from patients with salmonellosis

Realpe

Barba-León

Pérez-Montaño

et al. 2018

PeerJ

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“…Adicionalmente, los residuos de carne que permanecen en un molino no sanitizado permiten la proliferación bacteriana 34,35 . En un estudio realizado en Jalisco, México, se comparó la frecuencia del aislamiento de Salmonella en canales de res, trozos de carne de res y carne molida de res de carnicerías, encontrándose un mayor porcentaje de aislamiento en la carne molida de res, seguido de los trozos y finalmente en las canales (71, 39 y 18%, respectivamente); este estudio evidenció mayores concentraciones de Salmonella en la carne molida de res en comparación con trozos de carne, ya que reportaron como valores promedio 2,3 ± 1,1 Log NMP/25 g y 1,9 ± 0,9 Log NMP/25 g, respectivamente 36 . El riesgo de transmisión de Salmonella en las carnes molidas se incrementa cuando se consume cruda, ahumada o parcialmente cocida 37 .…”

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Frecuencia, susceptibilidad antimicrobiana y patrón de adherencia de Salmonella enterica aislada de carne de pollo, res y cerdo de la Ciudad de México

Villalpando-Guzmán

Vázquez-Quiñones

Natividad-Bonifacio

et al. 2017

Rev. chil. infectol.

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“…Quantitative and qualitative studies on microbiological safety are very important to the production of high quality meat products. The identification of potentially pathogenic microorganisms, such as Salmonella, in beef meat during slaughter operations contributes significantly to the implementation of quality monitoring programs and preventive measures, consequently reducing the risks to public health (Martínez-Chávez et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Survey of Salmonella spp. in beef meat for export at slaughterhouses in Brazil

et al. 2018

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The aim of the present study was to investigate the presence of Salmonella spp. in samples collected from beef meat at three points of the slaughter line (after skinning, washing and cooling) at three slaughterhouses in Brazil that export meat. Detection was based on ISO 6579:2002 and confirmed by PCR and qPCR. The isolates were typified using slide agglutination tests and PFGE. The antibiotic sensitivity profile was determined using the disk diffusion method. Contamination was detected in only one slaughterhouse. The overall frequency of contamination by Salmonella spp. was 6.7% of carcasses (6/90) and 2.6% of carcass surface samples (7/270). All isolates were confirmed by PCR and qPCR. The serological analysis and the PFGE showed a single profile: Typhimurium. The strains demonstrated 100% susceptibility to ampicillin, cefotaxime, ciprofloxacin, chloramphenicol, gentamicin and tetracycline. Positive carcasses after cooling pose a direct risk to consumers, since the meat is considered ready to be marketed after this process.

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Quantitative distribution of Salmonella spp. and Escherichia coli on beef carcasses and raw beef at retail establishments

Cited by 34 publications

References 16 publications

Genetic diversity and antimicrobial resistance ofSalmonellaserotypes recovered throughout the beef production chain and from patients with salmonellosis

Genetic diversity and antimicrobial resistance ofSalmonellaserotypes recovered throughout the beef production chain and from patients with salmonellosis

Frecuencia, susceptibilidad antimicrobiana y patrón de adherencia de Salmonella enterica aislada de carne de pollo, res y cerdo de la Ciudad de México

Survey of Salmonella spp. in beef meat for export at slaughterhouses in Brazil

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