Quantitative determination of the representative triterpenoids in the extracts of Ganoderma lucidum with different growth stages using high-performance liquid chromatography for evaluation of their 5α-reductase inhibitory properties

From 2000years of Ganoderma lucidum to recent developments in nutraceuticals

“…Care must be taken when applying and developing chemotaxic criteria as the type and quantity of the informative compound, for example triterpenes, can be influenced by the growth stage at which the product is harvested, which part of the fungus is harvested, as well as the growth or environmental conditions (Liu et al, 2012). Although chemotaxonomy may not be useful to allow for the definitive delineation of the Ganoderma species at this time, it will allow rough separation and perhaps more importantly, facilitate the quality control of Ganoderma products.…”

“…The isoprenes in triterpenes may form linear chains, or ringlike structures. GAs are a subtype of triterpenes with four cyclic and two linear isoprenes (Liu et al, 2012). There are over 140 species of GAs that have been identified from G. lucidum (Yue et al, 2010).…”

“…The different chemical compounds in R groups (R 1 -R 6 ) determine ganoderic acid subtype and its biochemical properties (figure adapted from (Liu et al, 2012)). bioenergetics electron transfer, as well as in response to exposure to some exogenous chemicals (Alphay, 2014;Yue et al, 2006).…”

Detection of Ganoderic Acid A in Ganoderma lingzhi by an Indirect Competitive Enzyme-Linked Immunosorbent Assay

“…Moreover, many samples can be analyzed without the need for pretreatment, and the developed icELISA combines both sensitivity and specificity for GAA. Considering that GAA is one of the major Ganoderma triterpenoids in commercially available G. lingzhi [12] and G. lucidum [13] in the market, a developed icELISA would be useful for quality control, where GAA content is used as an index. Furthermore, our study revealed that more than 50% ethanol is effective and suitable for the extraction of GAA, suggesting that the use of strong liquor is recommended to completely harness the potential of Ganoderma triterpenoids in daily life.…”

“…Among them, GAA (l " Fig. 1) is of great interest as it is abundantly present in Ganoderma species [12,13]. It has also been reported to suppress the growth and invasive behavior of the human breast cancer cell line MDA-MB-231.…”