Quantitative determination of the composition of individual pea seeds by fourier transform infrared photoacoustic spectroscopy

Abstract

Photoacoustic detection used in conjunction with multivariate partial least squares calibration showed a good predictive ability for the major components (starch, protein, lipid) of a single pea seed. Minimal sample preparation was required. The error of prediction achieved by photoacoustic spectroscopy was, for most components, close to the standard error of wet chemical methods but the technique had the advantage of a shorter analysis time and retained seed viability.

Study of Pea Accessions for Development of an Oilseed Pea

“…A relatively high lipid concentration was previously reported in pea seeds by Letzelter et al [11] and Bastianelli et al [12] at 9.7% and 35%, respectively, however none of our results exceeded 8% lipid concentration. Experiments by Lezelter et al [11] measured lipid content by photoacoustic detection, used in conjunction with multivariate partial least squares calibration whereas Bastianelli et al [12] used a lipid extraction technique using petroleum ether after acid hydrolysis. Such existing extraction methods do not specifically consider appropriate moisture removal, which seems to be a data-altering factor in reported lipid concentrations.…”

“…Only a few papers have been published on the lipid concentration in pea seeds. Earlier research has reported lipid contents ranging from 9.7% to 35%, respectively [11,12], while later research has reported lipid contents in field pea seeds ranging from 1% to 4% [13][14][15]. Existing reports using a water-based n-butanol extraction method showed that the most commonly found fatty acids in peas are linoleic acid in small and medium, and palmitic acid in larger seed accessions [16].…”

Fruits and Vegetables

“…In order to check the chemical composition of legume species as well as related isolates, several vibrational spectroscopy methods have been developed within recent years ( Jones et al , 1995 ). FTIR in conjunction with photoacoustic detection and PLS interpretation has proved to be a reliable tool to predict major components (starch, protein, lipids) occurring in single pea seeds ( Letzelter and Wilson, 1995 ). Linear regression of protein content calculated on the individual peak height measured at 1666 cm Ϫ 1 provided a correlation coeffi cient of 0.99.…”

Intra- and interspecific variation of cuticular hydrocarbon composition in two Ectatomma species (Hymenoptera: Formicidae) based on Fourier transform infrared photoacoustic spectroscopy

“…When absorption is high, the thermal wave has a high amplitude and the vibration of the microphone is increased. For more details on this method, see Letzelter et al (1995) and Belton et al (1988).…”