Quantitative determination of the cholinesterase inhibitor physostigmine in brain tissue samples using reversed-phase high-performance liquid chromatography

Biologically active components of Physostigma venenosum

“…In most methods, the mobile phases contain phosphate or acetate buffers (Table 1). In one early investigation, the authors [52] found that the use of sodium dodecyl sulphate (SDS) and NaClO 4 as the modifier of the mobile phase (pH 3.0) greatly improved peak shape and reproducibility of retention time of physostigmine in brain tissue samples.…”

Rapid High-Performance Liquid Chromatographic Determination of Physostigmine in Plasma

“…Although an enzymatic method (11) has the adequate sensitivity for the determination of physostigmine in h w n whole blood, i t requires a tedious sarrple preparation. Recently an HPLCmethod (12) with uv detection has been described for the assay of physostigmine in brain t i ssue . Methanol was purchased f r a n Burdick and Jackson Labs.…”