Quantitative determination of the anticancer prodrug combretastatin A1 phosphate (OXi4503, CA1P), the active CA1 and its glucuronide metabolites in human urine and of CA1 in plasma by HPLC with mass spectrometric detection

Stratford, Michael R.L.; Folkes, Lisa K.

doi:10.1016/j.jchromb.2012.03.040

Cited by 4 publications

(1 citation statement)

References 12 publications

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“…Here, we report the signal enhancement achievable for 13 C 1 ‐pyruvate and 13 C u ‐glucose‐d7 using direct injection into a tumor‐supplying artery. Additionally, in vivo monitoring of drug metabolism was evaluated for the first time by administering a custom synthesized hyperpolarized 13 C‐labeled version of the vascular disrupting pro‐drug, combretastatin A‐1‐diphosphate (CA1‐P) .…”

Section: Introductionmentioning

confidence: 99%

Direct arterial injection of hyperpolarized ¹³C‐labeled substrates into rat tumors for rapid MR detection of metabolism with minimal substrate dilution

Reynolds

Metcalf

Cochrane

et al. 2017

Magnetic Resonance in Med

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PurposeA rat model was developed to enable direct administration of hyperpolarized 13C‐labeled molecules into a tumor‐supplying artery for magnetic resonance spectroscopy (MRS) studies of tumor metabolism.MethodsRat P22 sarcomas were implanted into the right inguinal fat pad of BDIX rats such that the developing tumors received their principle blood supply directly from the right superior epigastric artery. Hyperpolarized 13C‐molecules were either infused directly to the tumor through the epigastric artery or systemically through the contralateral femoral vein. Spectroscopic data were obtained on a 7 Tesla preclinical scanner.ResultsIntra‐arterial infusion of hyperpolarized 13C‐pyruvate increased the pyruvate tumor signal by a factor of 4.6, compared with intravenous infusion, despite an approximately 7 times smaller total dose to the rat. Hyperpolarized glucose signal was detected at near‐physiological systemic blood concentration. Pyruvate to lactate but not glucose to lactate metabolism was detected in the tumor. Hyperpolarized 13C‐labeled combretastatin A1 diphosphate, a tumor vascular disrupting agent, showed an in vivo signal in the tumor.ConclusionsThe model maximizes tumor substrate/drug delivery and minimizes T1 relaxation signal losses in addition to systemic toxicity. Therefore, it permits metabolic studies of hyperpolarized substrates with relatively short T1 and opens up the possibility for preclinical studies of hyperpolarized drug molecules. Magn Reson Med 78:2116–2126, 2017. © 2017 The Authors Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Section: Introductionmentioning

confidence: 99%

Direct arterial injection of hyperpolarized ¹³C‐labeled substrates into rat tumors for rapid MR detection of metabolism with minimal substrate dilution

Reynolds

Metcalf

Cochrane

et al. 2017

Magnetic Resonance in Med

View full text Add to dashboard Cite

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The stilbene derivatives, nucleosides, and nucleosides modified by stilbene derivatives

Krawczyk

2019

Bioorganic Chemistry

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Synthesis of derivatives of methoxydibenzo[ b, f ]oxepine in the presence of sodium azide

et al. 2016

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Quantitative determination of the anticancer prodrug combretastatin A1 phosphate (OXi4503, CA1P), the active CA1 and its glucuronide metabolites in human urine and of CA1 in plasma by HPLC with mass spectrometric detection

Cited by 4 publications

References 12 publications

Direct arterial injection of hyperpolarized ¹³C‐labeled substrates into rat tumors for rapid MR detection of metabolism with minimal substrate dilution

Direct arterial injection of hyperpolarized ¹³C‐labeled substrates into rat tumors for rapid MR detection of metabolism with minimal substrate dilution

The stilbene derivatives, nucleosides, and nucleosides modified by stilbene derivatives

Synthesis of derivatives of methoxydibenzo[ b, f ]oxepine in the presence of sodium azide

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Quantitative determination of the anticancer prodrug combretastatin A1 phosphate (OXi4503, CA1P), the active CA1 and its glucuronide metabolites in human urine and of CA1 in plasma by HPLC with mass spectrometric detection

Cited by 4 publications

References 12 publications

Direct arterial injection of hyperpolarized 13C‐labeled substrates into rat tumors for rapid MR detection of metabolism with minimal substrate dilution

Direct arterial injection of hyperpolarized 13C‐labeled substrates into rat tumors for rapid MR detection of metabolism with minimal substrate dilution

The stilbene derivatives, nucleosides, and nucleosides modified by stilbene derivatives

Synthesis of derivatives of methoxydibenzo[ b, f ]oxepine in the presence of sodium azide

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Direct arterial injection of hyperpolarized ¹³C‐labeled substrates into rat tumors for rapid MR detection of metabolism with minimal substrate dilution

Direct arterial injection of hyperpolarized ¹³C‐labeled substrates into rat tumors for rapid MR detection of metabolism with minimal substrate dilution